Evaluation of three automated nucleic acid extraction systems for identification of respiratory viruses in clinical specimens by multiplex real-time PCR

Yoonjung Kim, Mi Soon Han, Juwon Kim, Aerin Kwon, Kyunga Lee

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

A total of 84 nasopharyngeal swab specimens were collected from 84 patients. Viral nucleic acid was extracted by three automated extraction systems: QIAcube (Qiagen, Germany), EZ1 Advanced XL (Qiagen), and MICROLAB Nimbus IVD (Hamilton, USA). Fourteen RNA viruses and two DNA viruses were detected using the Anyplex II RV16 Detection kit (Seegene, Republic of Korea). The EZ1 Advanced XL system demonstrated the best analytical sensitivity for all the three viral strains. The nucleic acids extracted by EZ1 Advanced XL showed higher positive rates for virus detection than the others. Meanwhile, the MICROLAB Nimbus IVD system was comprised of fully automated steps from nucleic extraction to PCR setup function that could reduce human errors. For the nucleic acids recovered from nasopharyngeal swab specimens, the QIAcube system showed the fewest false negative results and the best concordance rate, and it may be more suitable for detecting various viruses including RNA and DNA virus strains. Each system showed different sensitivity and specificity for detection of certain viral pathogens and demonstrated different characteristics such as turnaround time and sample capacity. Therefore, these factors should be considered when new nucleic acid extraction systems are introduced to the laboratory.

Original languageEnglish
Article number430650
JournalBioMed Research International
Volume2014
DOIs
Publication statusPublished - 2014 Jan 1

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Multiplex Polymerase Chain Reaction
Viruses
Respiratory System
Nucleic Acids
Real-Time Polymerase Chain Reaction
DNA Viruses
RNA Viruses
Republic of Korea
RNA
Turnaround time
DNA
Germany
Pathogens
Sensitivity and Specificity
Polymerase Chain Reaction

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

Cite this

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Evaluation of three automated nucleic acid extraction systems for identification of respiratory viruses in clinical specimens by multiplex real-time PCR. / Kim, Yoonjung; Han, Mi Soon; Kim, Juwon; Kwon, Aerin; Lee, Kyunga.

In: BioMed Research International, Vol. 2014, 430650, 01.01.2014.

Research output: Contribution to journalArticle

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