Evaluation of treadmill exercise effect on muscular lipid profiles of diabetic fatty rats by nanoflow liquid chromatography-tandem mass spectrometry

Jong Cheol Lee, Il Yong Kim, Yeri Son, Seul Kee Byeon, Dong Hyun Yoon, Jun Seok Son, Han Sol Song, Wook Song, Je Kyung Seong, Myeong Hee Moon

Research output: Contribution to journalArticle

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Abstract

We compare comprehensive quantitative profiling of lipids at the molecular level from skeletal muscle tissues (gastrocnemius and soleus) of Zucker diabetic fatty rats and Zucker lean control rats during treadmill exercise by nanoflow liquid chromatography-tandem mass spectrometry. Because type II diabetes is caused by decreased insulin sensitivity due to excess lipids accumulated in skeletal muscle tissue, lipidomic analysis of muscle tissues under treadmill exercise can help unveil the mechanism of lipid-associated insulin resistance. In total, 314 lipid species, including phospholipids, sphingolipids, ceramides, diacylglycerols (DAGs), and triacylglycerols (TAGs), were analyzed to examine diabetes-related lipid species and responses to treadmill exercise. Most lysophospholipid levels increased with diabetes. While DAG levels (10 from the gastrocnemius and 13 from the soleus) were >3-fold higher in diabetic rats, levels of most of these decreased after exercise in soleus but not in gastrocnemius. Levels of 5 highly abundant TAGs (52:1 and 54:3 in the gastrocnemius and 48:2, 50:2, and 52:4 in the soleus) displaying 2-fold increases in diabetic rats decreased after exercise in the soleus but not in the gastrocnemius in most cases. Thus, aerobic exercise has a stronger influence on lipid levels in the soleus than in the gastrocnemius in type 2 diabetic rats.

Original languageEnglish
Article number29617
JournalScientific reports
Volume6
DOIs
Publication statusPublished - 2016 Jul 8

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Tandem Mass Spectrometry
Liquid Chromatography
Lipids
Diglycerides
Muscles
Insulin Resistance
Skeletal Muscle
Triglycerides
Lysophospholipids
Zucker Rats
Sphingolipids
Ceramides
Type 2 Diabetes Mellitus
Phospholipids
Exercise

All Science Journal Classification (ASJC) codes

  • General

Cite this

Lee, Jong Cheol ; Kim, Il Yong ; Son, Yeri ; Byeon, Seul Kee ; Yoon, Dong Hyun ; Son, Jun Seok ; Song, Han Sol ; Song, Wook ; Seong, Je Kyung ; Moon, Myeong Hee. / Evaluation of treadmill exercise effect on muscular lipid profiles of diabetic fatty rats by nanoflow liquid chromatography-tandem mass spectrometry. In: Scientific reports. 2016 ; Vol. 6.
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Evaluation of treadmill exercise effect on muscular lipid profiles of diabetic fatty rats by nanoflow liquid chromatography-tandem mass spectrometry. / Lee, Jong Cheol; Kim, Il Yong; Son, Yeri; Byeon, Seul Kee; Yoon, Dong Hyun; Son, Jun Seok; Song, Han Sol; Song, Wook; Seong, Je Kyung; Moon, Myeong Hee.

In: Scientific reports, Vol. 6, 29617, 08.07.2016.

Research output: Contribution to journalArticle

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