Objective: Evaluation of altered urinary nucleosides before and after tumor removal of breast cancer (BCa). Design and methods: Targeted metabolite profiling of 14 urinary nucleosides was conducted with both pre- and post-operative female patients with BCa (n = 150, age: 46.6 ± 7.7 years), and female controls (n = 150, age: 46.8 ± 7.7 years) by liquid chromatography-tandem mass spectrometry coupled to on-line extraction. Results: Levels of modified nucleosides (5-hydroxymethyl-2′-deoxyuridine, P < 0.001; 8-hydroxy-2′-deoxyguanosine, P < 0.001; 1-methyladenosine, P < 0.02; N2,N2-dimethylguanosine, P < 0.001) were significantly higher in pre-operative patients than in both normal controls and post-operative patients. Conclusions: This approach could be used to further understand the pathogenesis of BCa as well as to evaluate the effects of medical treatment.
Bibliographical noteFunding Information:
This work was supported by an intramural grant from the Korean Institute of Science and Technology (KIST), and by grants from the National R&D Program of the Korean Ministry of Education, Science and Technology (MEST) and the Korean Science and Engineering Foundation (KOSEF).
All Science Journal Classification (ASJC) codes
- Clinical Biochemistry