Evidence supporting antioxidant action of adipose-derived stem cells: Protection of human dermal fibroblasts from oxidative stress

Won Serk Kim, Byung Soon Park, Hyung Ki Kim, Jeong Soo Park, Kea Jeung Kim, Joon Seok Choi, Suk Jae Chung, Dae Duk Kim, Jong Hyuk Sung

Research output: Contribution to journalArticle

152 Citations (Scopus)

Abstract

Background: Mesenchymal stem cells within the stromal-vascular fraction of subcutaneous adipose tissue, adipose-derived stem cells (ADSCs), produced soluble factors and they exhibit diverse pharmacological effects in skin biology. Objective: The present study examines the protective effect of ADSCs for human dermal fibroblasts (HDFs) through anti-oxidation in a tert-butyl hydroperoxide (tbOOH) induced oxidative injury model. Methods and results: The conditioned medium of ADSCs (ADSC-CM) was harvested and tested for antioxidant action. ADSC-CM had an antioxidant effect as potent as 100 μM ascorbic acid and various antioxidant proteins were detected in ADSC-CM by proteomic analysis. Morphological change and cell survival assay revealed that incubation with ADSC-CM aided HDFs to resist free radicals induced by tbOOH. In addition, activities of superoxide dismutase and glutathione peroxidase were enhanced in the ADSC-CM treated HDFs which confirmed the study hypothesis that ADSCs protect HDFs through antioxidant action. In a cell cycle analysis, ADSC-CM treatment reversed the apoptotic cell death induced by tbOOH and caused a decrease of sub-G1 cells with respect to untreated cells. The anti-apoptotic effect of ADSC-CM was also reproduced by caspase-3 activity assay. Conclusion: These results suggest that ADSCs have potent antioxidant activity and protect HDFs from oxidative injury by decreasing apoptotic cells. Therefore, ADSCs and ADSC-CM are good candidates for control and prevention of skin damage from free radicals in various skin conditions.

Original languageEnglish
Pages (from-to)133-142
Number of pages10
JournalJournal of Dermatological Science
Volume49
Issue number2
DOIs
Publication statusPublished - 2008 Feb 1

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Oxidative stress
Cytoprotection
Fibroblasts
Stem cells
Oxidative Stress
Stem Cells
Antioxidants
Skin
Cells
Free Radicals
Assays
tert-Butylhydroperoxide
Subcutaneous Fat
Wounds and Injuries
Conditioned Culture Medium
Glutathione Peroxidase
Mesenchymal Stromal Cells
Cell death
Human Activities
Caspase 3

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Dermatology

Cite this

Kim, Won Serk ; Park, Byung Soon ; Kim, Hyung Ki ; Park, Jeong Soo ; Kim, Kea Jeung ; Choi, Joon Seok ; Chung, Suk Jae ; Kim, Dae Duk ; Sung, Jong Hyuk. / Evidence supporting antioxidant action of adipose-derived stem cells : Protection of human dermal fibroblasts from oxidative stress. In: Journal of Dermatological Science. 2008 ; Vol. 49, No. 2. pp. 133-142.
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abstract = "Background: Mesenchymal stem cells within the stromal-vascular fraction of subcutaneous adipose tissue, adipose-derived stem cells (ADSCs), produced soluble factors and they exhibit diverse pharmacological effects in skin biology. Objective: The present study examines the protective effect of ADSCs for human dermal fibroblasts (HDFs) through anti-oxidation in a tert-butyl hydroperoxide (tbOOH) induced oxidative injury model. Methods and results: The conditioned medium of ADSCs (ADSC-CM) was harvested and tested for antioxidant action. ADSC-CM had an antioxidant effect as potent as 100 μM ascorbic acid and various antioxidant proteins were detected in ADSC-CM by proteomic analysis. Morphological change and cell survival assay revealed that incubation with ADSC-CM aided HDFs to resist free radicals induced by tbOOH. In addition, activities of superoxide dismutase and glutathione peroxidase were enhanced in the ADSC-CM treated HDFs which confirmed the study hypothesis that ADSCs protect HDFs through antioxidant action. In a cell cycle analysis, ADSC-CM treatment reversed the apoptotic cell death induced by tbOOH and caused a decrease of sub-G1 cells with respect to untreated cells. The anti-apoptotic effect of ADSC-CM was also reproduced by caspase-3 activity assay. Conclusion: These results suggest that ADSCs have potent antioxidant activity and protect HDFs from oxidative injury by decreasing apoptotic cells. Therefore, ADSCs and ADSC-CM are good candidates for control and prevention of skin damage from free radicals in various skin conditions.",
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Evidence supporting antioxidant action of adipose-derived stem cells : Protection of human dermal fibroblasts from oxidative stress. / Kim, Won Serk; Park, Byung Soon; Kim, Hyung Ki; Park, Jeong Soo; Kim, Kea Jeung; Choi, Joon Seok; Chung, Suk Jae; Kim, Dae Duk; Sung, Jong Hyuk.

In: Journal of Dermatological Science, Vol. 49, No. 2, 01.02.2008, p. 133-142.

Research output: Contribution to journalArticle

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T1 - Evidence supporting antioxidant action of adipose-derived stem cells

T2 - Protection of human dermal fibroblasts from oxidative stress

AU - Kim, Won Serk

AU - Park, Byung Soon

AU - Kim, Hyung Ki

AU - Park, Jeong Soo

AU - Kim, Kea Jeung

AU - Choi, Joon Seok

AU - Chung, Suk Jae

AU - Kim, Dae Duk

AU - Sung, Jong Hyuk

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N2 - Background: Mesenchymal stem cells within the stromal-vascular fraction of subcutaneous adipose tissue, adipose-derived stem cells (ADSCs), produced soluble factors and they exhibit diverse pharmacological effects in skin biology. Objective: The present study examines the protective effect of ADSCs for human dermal fibroblasts (HDFs) through anti-oxidation in a tert-butyl hydroperoxide (tbOOH) induced oxidative injury model. Methods and results: The conditioned medium of ADSCs (ADSC-CM) was harvested and tested for antioxidant action. ADSC-CM had an antioxidant effect as potent as 100 μM ascorbic acid and various antioxidant proteins were detected in ADSC-CM by proteomic analysis. Morphological change and cell survival assay revealed that incubation with ADSC-CM aided HDFs to resist free radicals induced by tbOOH. In addition, activities of superoxide dismutase and glutathione peroxidase were enhanced in the ADSC-CM treated HDFs which confirmed the study hypothesis that ADSCs protect HDFs through antioxidant action. In a cell cycle analysis, ADSC-CM treatment reversed the apoptotic cell death induced by tbOOH and caused a decrease of sub-G1 cells with respect to untreated cells. The anti-apoptotic effect of ADSC-CM was also reproduced by caspase-3 activity assay. Conclusion: These results suggest that ADSCs have potent antioxidant activity and protect HDFs from oxidative injury by decreasing apoptotic cells. Therefore, ADSCs and ADSC-CM are good candidates for control and prevention of skin damage from free radicals in various skin conditions.

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