Cellulose-producing Acetobacter xylinum has been known to secrete a cellulose-hydrolyzing β-1,4-endoglucanase (CMCax). When antibodies to recombinant CMCax were added to the culture medium, the formation of cellulose fiber was severely inhibited. Western blot analysis using the antibody showed that this enzyme was secreted into the medium even by a cellulose non-producing strain (Cel−). These results indicate that β-1,4-endoglucanase in the medium plays a critical role in the formation of cellulose fiber by the microorganism.
All Science Journal Classification (ASJC) codes
- Analytical Chemistry
- Applied Microbiology and Biotechnology
- Molecular Biology
- Organic Chemistry