Evidences for possible involvement of Rhp51 protein in mitotic events including chromosome segregation

Yeun Kyu Jang, Y. H. Jin, Y. S. Shim, M. J. Kim, E. J. Yoo, R. H. Seong, S. H. Hong, S. D. Park

Research output: Contribution to journalArticle

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Abstract

To understand the role of the Rhp51 protein in Schizosaccharomyces pombe, we examined the phenotypes of the null mutant for the rhp51+ gene. Unlike Saccharomyces cerevisiae rad51 mutants, S. pombe rhp51 mutants (rhp51Δ cells) displayed slow growth and heterogeneity in cell size, indicating perturbation of the cell cycle. Furthermore, many aberrant nuclear structures found in 4',6'-diamidino-2-phenylindole (DAPI)-stained rhp51Δ cells and the caffeine hypersensitivity of the mutant cells suggested an involvement of the Rhp51 protein in normal chromosome segregation. These data suggested that the Rhp51 proteins were required for normal cell growth as well as a DNA repair pathway. Moreover, rhp51Δ mutants showed a considerable sensitivity to ultraviolet (UV) light-irradiation as well as methyl methanesulfonate (MMS) treatment, indicating that the Rhp51 proteins are involved in both the active excision mechanism of UV-induced DNA damage and recombinational repair in S. pombe. Taken together, we suggest that the role(s) of the Rhp51 protein in S. pombe may be different from those of Rad51 in S. cerevisiae.

Original languageEnglish
Pages (from-to)329-337
Number of pages9
JournalBiochemistry and Molecular Biology International
Volume37
Issue number2
Publication statusPublished - 1995 Dec 1

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Chromosome Segregation
Chromosomes
Schizosaccharomyces pombe Proteins
Schizosaccharomyces
Saccharomyces cerevisiae
Proteins
Yeast
Cell Enlargement
Methyl Methanesulfonate
Repair
Ultraviolet Rays
Caffeine
DNA Repair
DNA Damage
DNA
Cell growth
Cell Cycle
Hypersensitivity
Phenotype
Genes

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Genetics

Cite this

Jang, Y. K., Jin, Y. H., Shim, Y. S., Kim, M. J., Yoo, E. J., Seong, R. H., ... Park, S. D. (1995). Evidences for possible involvement of Rhp51 protein in mitotic events including chromosome segregation. Biochemistry and Molecular Biology International, 37(2), 329-337.
Jang, Yeun Kyu ; Jin, Y. H. ; Shim, Y. S. ; Kim, M. J. ; Yoo, E. J. ; Seong, R. H. ; Hong, S. H. ; Park, S. D. / Evidences for possible involvement of Rhp51 protein in mitotic events including chromosome segregation. In: Biochemistry and Molecular Biology International. 1995 ; Vol. 37, No. 2. pp. 329-337.
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abstract = "To understand the role of the Rhp51 protein in Schizosaccharomyces pombe, we examined the phenotypes of the null mutant for the rhp51+ gene. Unlike Saccharomyces cerevisiae rad51 mutants, S. pombe rhp51 mutants (rhp51Δ cells) displayed slow growth and heterogeneity in cell size, indicating perturbation of the cell cycle. Furthermore, many aberrant nuclear structures found in 4',6'-diamidino-2-phenylindole (DAPI)-stained rhp51Δ cells and the caffeine hypersensitivity of the mutant cells suggested an involvement of the Rhp51 protein in normal chromosome segregation. These data suggested that the Rhp51 proteins were required for normal cell growth as well as a DNA repair pathway. Moreover, rhp51Δ mutants showed a considerable sensitivity to ultraviolet (UV) light-irradiation as well as methyl methanesulfonate (MMS) treatment, indicating that the Rhp51 proteins are involved in both the active excision mechanism of UV-induced DNA damage and recombinational repair in S. pombe. Taken together, we suggest that the role(s) of the Rhp51 protein in S. pombe may be different from those of Rad51 in S. cerevisiae.",
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Jang, YK, Jin, YH, Shim, YS, Kim, MJ, Yoo, EJ, Seong, RH, Hong, SH & Park, SD 1995, 'Evidences for possible involvement of Rhp51 protein in mitotic events including chromosome segregation', Biochemistry and Molecular Biology International, vol. 37, no. 2, pp. 329-337.

Evidences for possible involvement of Rhp51 protein in mitotic events including chromosome segregation. / Jang, Yeun Kyu; Jin, Y. H.; Shim, Y. S.; Kim, M. J.; Yoo, E. J.; Seong, R. H.; Hong, S. H.; Park, S. D.

In: Biochemistry and Molecular Biology International, Vol. 37, No. 2, 01.12.1995, p. 329-337.

Research output: Contribution to journalArticle

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AU - Jang, Yeun Kyu

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AU - Shim, Y. S.

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AU - Yoo, E. J.

AU - Seong, R. H.

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AU - Park, S. D.

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N2 - To understand the role of the Rhp51 protein in Schizosaccharomyces pombe, we examined the phenotypes of the null mutant for the rhp51+ gene. Unlike Saccharomyces cerevisiae rad51 mutants, S. pombe rhp51 mutants (rhp51Δ cells) displayed slow growth and heterogeneity in cell size, indicating perturbation of the cell cycle. Furthermore, many aberrant nuclear structures found in 4',6'-diamidino-2-phenylindole (DAPI)-stained rhp51Δ cells and the caffeine hypersensitivity of the mutant cells suggested an involvement of the Rhp51 protein in normal chromosome segregation. These data suggested that the Rhp51 proteins were required for normal cell growth as well as a DNA repair pathway. Moreover, rhp51Δ mutants showed a considerable sensitivity to ultraviolet (UV) light-irradiation as well as methyl methanesulfonate (MMS) treatment, indicating that the Rhp51 proteins are involved in both the active excision mechanism of UV-induced DNA damage and recombinational repair in S. pombe. Taken together, we suggest that the role(s) of the Rhp51 protein in S. pombe may be different from those of Rad51 in S. cerevisiae.

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