Existence of glioma stroma mesenchymal stemlike cells in Korean glioma specimens

Young Goo Kim, Soyoun Jeon, Ga Yeong Sin, Jin Kyoung Shim, Bo Kyung Kim, Hye Jin Shin, Ji Hyun Lee, yongmin Huh, Su Jae Lee, Eui Hyun Kim, Eun Kyung Park, SeHoon Kim, Jong Hee Chang, Dong Seok Kim, Sun Ho Kim, Yong Kil Hong, Seok-Gu Kang, Frederick F. Lang

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Purpose: It was presented that mesenchymal stem cells (MSCs) can be isolated from western glioma specimens. However, whether MSCs exist in glioma specimens of different ethnicities is unknown. To verify the existence of MSCs in an independent cohort, we undertook studies to isolate MSCs from a group of Korean patients. We hypothesized that cells resembling MSCs that were deemed mesenchymal stemlike cells (MSLCs) exist in an independent cohort of Korean gliomas. Methods: We cultured fresh glioma specimens using the protocols used for culturing MSCs. The cultured cells were analyzed with fluorescence-activated cell sorting (FACS) for surface markers associated with MSCs. Cultured cells were exposed to mesenchymal differentiation conditions. To presume possible locations of MSLCs in the glioma, sections of glioma were analyzed by immunofluorescent labeling for CD105, CD31, and NG2. Results: From nine of 31 glioma specimens, we isolated cells resembling MSCs, which were deemed Korean glioma stroma MSLCs (KGS-MSLCs). KGS-MSLCs were spindle shaped and adherent to plastic. KGS-MSLCs had similar surface markers to MSCs (CD105 + , CD90 + , CD73 + , and CD45 - ). KGS-MSLCs were capable of mesenchymal differentiation and might be located around endothelial cells, pericytes, and in a disorganized perivascular area inside glioma stroma. Conclusions: We found that cells resembling MSCs indeed exist in an independent cohort of glioma patients, as presented in western populations. We could presume that the possible location of KGS-MSLCs was in perivascular area or in glioma stroma that was a disorganized vascular niche. It might be possible that KGS-MSLCs could be one of constituent of stroma of glioma microenvironment.

Original languageEnglish
Pages (from-to)549-563
Number of pages15
JournalChild's Nervous System
Volume29
Issue number4
DOIs
Publication statusPublished - 2013 Apr 1

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Glioma
Mesenchymal Stromal Cells
Cultured Cells
Pericytes
Plastics
Blood Vessels
Flow Cytometry

All Science Journal Classification (ASJC) codes

  • Pediatrics, Perinatology, and Child Health
  • Clinical Neurology

Cite this

Kim, Y. G., Jeon, S., Sin, G. Y., Shim, J. K., Kim, B. K., Shin, H. J., ... Lang, F. F. (2013). Existence of glioma stroma mesenchymal stemlike cells in Korean glioma specimens. Child's Nervous System, 29(4), 549-563. https://doi.org/10.1007/s00381-012-1988-1
Kim, Young Goo ; Jeon, Soyoun ; Sin, Ga Yeong ; Shim, Jin Kyoung ; Kim, Bo Kyung ; Shin, Hye Jin ; Lee, Ji Hyun ; Huh, yongmin ; Lee, Su Jae ; Kim, Eui Hyun ; Park, Eun Kyung ; Kim, SeHoon ; Chang, Jong Hee ; Kim, Dong Seok ; Kim, Sun Ho ; Hong, Yong Kil ; Kang, Seok-Gu ; Lang, Frederick F. / Existence of glioma stroma mesenchymal stemlike cells in Korean glioma specimens. In: Child's Nervous System. 2013 ; Vol. 29, No. 4. pp. 549-563.
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title = "Existence of glioma stroma mesenchymal stemlike cells in Korean glioma specimens",
abstract = "Purpose: It was presented that mesenchymal stem cells (MSCs) can be isolated from western glioma specimens. However, whether MSCs exist in glioma specimens of different ethnicities is unknown. To verify the existence of MSCs in an independent cohort, we undertook studies to isolate MSCs from a group of Korean patients. We hypothesized that cells resembling MSCs that were deemed mesenchymal stemlike cells (MSLCs) exist in an independent cohort of Korean gliomas. Methods: We cultured fresh glioma specimens using the protocols used for culturing MSCs. The cultured cells were analyzed with fluorescence-activated cell sorting (FACS) for surface markers associated with MSCs. Cultured cells were exposed to mesenchymal differentiation conditions. To presume possible locations of MSLCs in the glioma, sections of glioma were analyzed by immunofluorescent labeling for CD105, CD31, and NG2. Results: From nine of 31 glioma specimens, we isolated cells resembling MSCs, which were deemed Korean glioma stroma MSLCs (KGS-MSLCs). KGS-MSLCs were spindle shaped and adherent to plastic. KGS-MSLCs had similar surface markers to MSCs (CD105 + , CD90 + , CD73 + , and CD45 - ). KGS-MSLCs were capable of mesenchymal differentiation and might be located around endothelial cells, pericytes, and in a disorganized perivascular area inside glioma stroma. Conclusions: We found that cells resembling MSCs indeed exist in an independent cohort of glioma patients, as presented in western populations. We could presume that the possible location of KGS-MSLCs was in perivascular area or in glioma stroma that was a disorganized vascular niche. It might be possible that KGS-MSLCs could be one of constituent of stroma of glioma microenvironment.",
author = "Kim, {Young Goo} and Soyoun Jeon and Sin, {Ga Yeong} and Shim, {Jin Kyoung} and Kim, {Bo Kyung} and Shin, {Hye Jin} and Lee, {Ji Hyun} and yongmin Huh and Lee, {Su Jae} and Kim, {Eui Hyun} and Park, {Eun Kyung} and SeHoon Kim and Chang, {Jong Hee} and Kim, {Dong Seok} and Kim, {Sun Ho} and Hong, {Yong Kil} and Seok-Gu Kang and Lang, {Frederick F.}",
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Kim, YG, Jeon, S, Sin, GY, Shim, JK, Kim, BK, Shin, HJ, Lee, JH, Huh, Y, Lee, SJ, Kim, EH, Park, EK, Kim, S, Chang, JH, Kim, DS, Kim, SH, Hong, YK, Kang, S-G & Lang, FF 2013, 'Existence of glioma stroma mesenchymal stemlike cells in Korean glioma specimens', Child's Nervous System, vol. 29, no. 4, pp. 549-563. https://doi.org/10.1007/s00381-012-1988-1

Existence of glioma stroma mesenchymal stemlike cells in Korean glioma specimens. / Kim, Young Goo; Jeon, Soyoun; Sin, Ga Yeong; Shim, Jin Kyoung; Kim, Bo Kyung; Shin, Hye Jin; Lee, Ji Hyun; Huh, yongmin; Lee, Su Jae; Kim, Eui Hyun; Park, Eun Kyung; Kim, SeHoon; Chang, Jong Hee; Kim, Dong Seok; Kim, Sun Ho; Hong, Yong Kil; Kang, Seok-Gu; Lang, Frederick F.

In: Child's Nervous System, Vol. 29, No. 4, 01.04.2013, p. 549-563.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Existence of glioma stroma mesenchymal stemlike cells in Korean glioma specimens

AU - Kim, Young Goo

AU - Jeon, Soyoun

AU - Sin, Ga Yeong

AU - Shim, Jin Kyoung

AU - Kim, Bo Kyung

AU - Shin, Hye Jin

AU - Lee, Ji Hyun

AU - Huh, yongmin

AU - Lee, Su Jae

AU - Kim, Eui Hyun

AU - Park, Eun Kyung

AU - Kim, SeHoon

AU - Chang, Jong Hee

AU - Kim, Dong Seok

AU - Kim, Sun Ho

AU - Hong, Yong Kil

AU - Kang, Seok-Gu

AU - Lang, Frederick F.

PY - 2013/4/1

Y1 - 2013/4/1

N2 - Purpose: It was presented that mesenchymal stem cells (MSCs) can be isolated from western glioma specimens. However, whether MSCs exist in glioma specimens of different ethnicities is unknown. To verify the existence of MSCs in an independent cohort, we undertook studies to isolate MSCs from a group of Korean patients. We hypothesized that cells resembling MSCs that were deemed mesenchymal stemlike cells (MSLCs) exist in an independent cohort of Korean gliomas. Methods: We cultured fresh glioma specimens using the protocols used for culturing MSCs. The cultured cells were analyzed with fluorescence-activated cell sorting (FACS) for surface markers associated with MSCs. Cultured cells were exposed to mesenchymal differentiation conditions. To presume possible locations of MSLCs in the glioma, sections of glioma were analyzed by immunofluorescent labeling for CD105, CD31, and NG2. Results: From nine of 31 glioma specimens, we isolated cells resembling MSCs, which were deemed Korean glioma stroma MSLCs (KGS-MSLCs). KGS-MSLCs were spindle shaped and adherent to plastic. KGS-MSLCs had similar surface markers to MSCs (CD105 + , CD90 + , CD73 + , and CD45 - ). KGS-MSLCs were capable of mesenchymal differentiation and might be located around endothelial cells, pericytes, and in a disorganized perivascular area inside glioma stroma. Conclusions: We found that cells resembling MSCs indeed exist in an independent cohort of glioma patients, as presented in western populations. We could presume that the possible location of KGS-MSLCs was in perivascular area or in glioma stroma that was a disorganized vascular niche. It might be possible that KGS-MSLCs could be one of constituent of stroma of glioma microenvironment.

AB - Purpose: It was presented that mesenchymal stem cells (MSCs) can be isolated from western glioma specimens. However, whether MSCs exist in glioma specimens of different ethnicities is unknown. To verify the existence of MSCs in an independent cohort, we undertook studies to isolate MSCs from a group of Korean patients. We hypothesized that cells resembling MSCs that were deemed mesenchymal stemlike cells (MSLCs) exist in an independent cohort of Korean gliomas. Methods: We cultured fresh glioma specimens using the protocols used for culturing MSCs. The cultured cells were analyzed with fluorescence-activated cell sorting (FACS) for surface markers associated with MSCs. Cultured cells were exposed to mesenchymal differentiation conditions. To presume possible locations of MSLCs in the glioma, sections of glioma were analyzed by immunofluorescent labeling for CD105, CD31, and NG2. Results: From nine of 31 glioma specimens, we isolated cells resembling MSCs, which were deemed Korean glioma stroma MSLCs (KGS-MSLCs). KGS-MSLCs were spindle shaped and adherent to plastic. KGS-MSLCs had similar surface markers to MSCs (CD105 + , CD90 + , CD73 + , and CD45 - ). KGS-MSLCs were capable of mesenchymal differentiation and might be located around endothelial cells, pericytes, and in a disorganized perivascular area inside glioma stroma. Conclusions: We found that cells resembling MSCs indeed exist in an independent cohort of glioma patients, as presented in western populations. We could presume that the possible location of KGS-MSLCs was in perivascular area or in glioma stroma that was a disorganized vascular niche. It might be possible that KGS-MSLCs could be one of constituent of stroma of glioma microenvironment.

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U2 - 10.1007/s00381-012-1988-1

DO - 10.1007/s00381-012-1988-1

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SN - 0256-7040

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