Exogenous pentraxin-3 inhibits the reactive oxygen species-mitochondrial and apoptosis pathway in acute kidney injury

Hyung Ho Lee, Sook Young Kim, Joon Chae Na, Young Eun Yoon, WoongKyu Han

Research output: Contribution to journalArticle

Abstract

Pentraxin-3 (PTX3) is a long-form member of the pentraxin family of proteins that has been studied in inflammatory diseases and in various organs. We found that PTX3 protects kidney cells during ischemia and proinflammatory acute kidney injury. The aim of this study was to develop an in vitro experimental model of acute kidney injury and to analyze the protective mechanism of exogenous recombinant PTX3. In this study, cells of the HK-2 renal tubular cell line were treated with a calcium ionophore (A23187), which induced injury by increasing intracellular calcium concentrations and inducing calpain activity and the generation of reactive oxygen species. Exposure of cells to PTX3 significantly attenuated these effects. In addition, the activity of caspase-3 and PARP-1 were decreased in ischemic cells exposed to exogenous recombinant PTX3. PTX3 stabilized the mitochondrial membrane potential and suppressed apoptosis, resulting in the protection of renal tubular cells from ischemic injury.

Original languageEnglish
Article numbere0195758
JournalPloS one
Volume13
Issue number4
DOIs
Publication statusPublished - 2018 Apr 1

Fingerprint

kidney cells
Acute Kidney Injury
reactive oxygen species
Reactive Oxygen Species
apoptosis
kidneys
Apoptosis
calcium
calpain
ionophores
cells
caspase-3
ischemia
Kidney
membrane potential
cell lines
Calpain
Calcium Ionophores
Mitochondrial Membrane Potential
Calcimycin

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Lee, Hyung Ho ; Kim, Sook Young ; Na, Joon Chae ; Yoon, Young Eun ; Han, WoongKyu. / Exogenous pentraxin-3 inhibits the reactive oxygen species-mitochondrial and apoptosis pathway in acute kidney injury. In: PloS one. 2018 ; Vol. 13, No. 4.
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Exogenous pentraxin-3 inhibits the reactive oxygen species-mitochondrial and apoptosis pathway in acute kidney injury. / Lee, Hyung Ho; Kim, Sook Young; Na, Joon Chae; Yoon, Young Eun; Han, WoongKyu.

In: PloS one, Vol. 13, No. 4, e0195758, 01.04.2018.

Research output: Contribution to journalArticle

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