Abstract
Intimal hyperplasia is an excessive ingrowth of tissue resulting in chronic structural lesions commonly found at sites of atherosclerotic lesions, arterial angioplasty, vascular graft anastomoses, and other vascular abnormalities. Epigallocatechin-3-O-gallate (EGCG) was shown to elicit antioxidant, anti-proliferative, and anti-thrombogenic effects. In this study, we used an electrospinning technique to synthesize EGCG-eluting biodegradable poly(l-lactide glycolic acid) (PLGA) fiber sheets for local delivery of EGCG and investigated the effect of their exovascular application on intimal hyperplasia following balloon-induced abdominal aorta injury in a rabbit experimental model. The morphology of the composite sheets was characterized using scanning electron microscopy and Fourier transform-infrared spectroscopy. EGCG was loaded and dispersed into the PLGA-based electrospun fibers. The EGCG-loaded PLGA sheets exhibited sustained EGCG release following the initial 24 h of burst release in phosphate-buffered saline. In vivo studies demonstrated significant inhibition of intimal hyperplasia following the application of the EGCG-eluting electrospun PLGA fiber sheets, compared with vehicle PLGA controls. In conclusion, our results show that exovascular application of EGCG-eluting PLGA electrospun fiber sheets may provide a useful system for the effective local delivery of drugs for the prevention of intimal hyperplasia.
| Original language | English |
|---|---|
| Article number | 055010 |
| Journal | Biomedical Materials (Bristol) |
| Volume | 10 |
| Issue number | 5 |
| DOIs | |
| Publication status | Published - 2015 Sep 21 |
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All Science Journal Classification (ASJC) codes
- Bioengineering
- Biomaterials
- Biomedical Engineering
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Exovascular application of epigallocatechin-3-O-gallate-releasing electrospun poly(l-lactide glycolic acid) fiber sheets to reduce intimal hyperplasia in injured abdominal aorta. / Lee, Mi Hee; Kwon, Byeong Ju; Koo, Min Ah; Jang, Eui Hwa; Seon, Gyeung Mi; Park, Jongchul.
In: Biomedical Materials (Bristol), Vol. 10, No. 5, 055010, 21.09.2015.Research output: Contribution to journal › Article
TY - JOUR
T1 - Exovascular application of epigallocatechin-3-O-gallate-releasing electrospun poly(l-lactide glycolic acid) fiber sheets to reduce intimal hyperplasia in injured abdominal aorta
AU - Lee, Mi Hee
AU - Kwon, Byeong Ju
AU - Koo, Min Ah
AU - Jang, Eui Hwa
AU - Seon, Gyeung Mi
AU - Park, Jongchul
PY - 2015/9/21
Y1 - 2015/9/21
N2 - Intimal hyperplasia is an excessive ingrowth of tissue resulting in chronic structural lesions commonly found at sites of atherosclerotic lesions, arterial angioplasty, vascular graft anastomoses, and other vascular abnormalities. Epigallocatechin-3-O-gallate (EGCG) was shown to elicit antioxidant, anti-proliferative, and anti-thrombogenic effects. In this study, we used an electrospinning technique to synthesize EGCG-eluting biodegradable poly(l-lactide glycolic acid) (PLGA) fiber sheets for local delivery of EGCG and investigated the effect of their exovascular application on intimal hyperplasia following balloon-induced abdominal aorta injury in a rabbit experimental model. The morphology of the composite sheets was characterized using scanning electron microscopy and Fourier transform-infrared spectroscopy. EGCG was loaded and dispersed into the PLGA-based electrospun fibers. The EGCG-loaded PLGA sheets exhibited sustained EGCG release following the initial 24 h of burst release in phosphate-buffered saline. In vivo studies demonstrated significant inhibition of intimal hyperplasia following the application of the EGCG-eluting electrospun PLGA fiber sheets, compared with vehicle PLGA controls. In conclusion, our results show that exovascular application of EGCG-eluting PLGA electrospun fiber sheets may provide a useful system for the effective local delivery of drugs for the prevention of intimal hyperplasia.
AB - Intimal hyperplasia is an excessive ingrowth of tissue resulting in chronic structural lesions commonly found at sites of atherosclerotic lesions, arterial angioplasty, vascular graft anastomoses, and other vascular abnormalities. Epigallocatechin-3-O-gallate (EGCG) was shown to elicit antioxidant, anti-proliferative, and anti-thrombogenic effects. In this study, we used an electrospinning technique to synthesize EGCG-eluting biodegradable poly(l-lactide glycolic acid) (PLGA) fiber sheets for local delivery of EGCG and investigated the effect of their exovascular application on intimal hyperplasia following balloon-induced abdominal aorta injury in a rabbit experimental model. The morphology of the composite sheets was characterized using scanning electron microscopy and Fourier transform-infrared spectroscopy. EGCG was loaded and dispersed into the PLGA-based electrospun fibers. The EGCG-loaded PLGA sheets exhibited sustained EGCG release following the initial 24 h of burst release in phosphate-buffered saline. In vivo studies demonstrated significant inhibition of intimal hyperplasia following the application of the EGCG-eluting electrospun PLGA fiber sheets, compared with vehicle PLGA controls. In conclusion, our results show that exovascular application of EGCG-eluting PLGA electrospun fiber sheets may provide a useful system for the effective local delivery of drugs for the prevention of intimal hyperplasia.
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U2 - 10.1088/1748-6041/10/5/055010
DO - 10.1088/1748-6041/10/5/055010
M3 - Article
C2 - 26391656
AN - SCOPUS:84947976137
VL - 10
JO - Biomedical Materials (Bristol)
JF - Biomedical Materials (Bristol)
SN - 1748-6041
IS - 5
M1 - 055010
ER -