Expanding the genetic code of Mus musculus

Songmi Han, Aerin Yang, Soonjang Lee, Han Woong Lee, Chan Bae Park, Hee Sung Park

Research output: Contribution to journalArticlepeer-review

34 Citations (Scopus)

Abstract

Here we report the expansion of the genetic code of Mus musculus with various unnatural amino acids including Ne-acetyl-lysine. Stable integration of transgenes encoding an engineered Nϵ-acetyl-lysyl-tRNA synthetase (AcKRS)/tRNAPyl pair into the mouse genome enables site-specific incorporation of unnatural amino acids into a target protein in response to the amber codon. We demonstrate temporal and spatial control of protein acetylation in various organs of the transgenic mouse using a recombinant green fluorescent protein (GFPuv) as a model protein. This strategy will provide a powerful tool for systematic in vivo study of cellular proteins in the most commonly used mammalian model organism for human physiology and disease.

Original languageEnglish
Article number14568
JournalNature communications
Volume8
DOIs
Publication statusPublished - 2017 Feb 21

Bibliographical note

Funding Information:
This work was supported by grants from the National Research Foundation of Korea (KAIST Systems Healthcare, 2011-0020322, and 2014M3A6A4075060) to H.-S.P., and the Intelligent Synthetic Biology Center of Global Frontier Project funded by the Ministry of Science, ICT & Future Planning (2014M3A6A8066439) and Ministry of Food and Drug Safety (14182MFDS978) awarded to C.B.P.

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Physics and Astronomy(all)

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