Expanding the genetic code of Mus musculus

Songmi Han, Aerin Yang, Soonjang Lee, Han Woong Lee, Chan Bae Park, Hee Sung Park

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Here we report the expansion of the genetic code of Mus musculus with various unnatural amino acids including Ne-acetyl-lysine. Stable integration of transgenes encoding an engineered Nϵ-acetyl-lysyl-tRNA synthetase (AcKRS)/tRNAPyl pair into the mouse genome enables site-specific incorporation of unnatural amino acids into a target protein in response to the amber codon. We demonstrate temporal and spatial control of protein acetylation in various organs of the transgenic mouse using a recombinant green fluorescent protein (GFPuv) as a model protein. This strategy will provide a powerful tool for systematic in vivo study of cellular proteins in the most commonly used mammalian model organism for human physiology and disease.

Original languageEnglish
Article number14568
JournalNature Communications
Volume8
DOIs
Publication statusPublished - 2017 Feb 21

Fingerprint

genetic code
Genetic Code
proteins
Lysine-tRNA Ligase
Proteins
mice
amino acids
Amino Acids
Acetylation
Terminator Codon
Physiology
acetylation
Green Fluorescent Proteins
Transgenes
Recombinant Proteins
physiology
Transgenic Mice
genome
lysine
Lysine

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Physics and Astronomy(all)

Cite this

Han, S., Yang, A., Lee, S., Lee, H. W., Park, C. B., & Park, H. S. (2017). Expanding the genetic code of Mus musculus. Nature Communications, 8, [14568]. https://doi.org/10.1038/ncomms14568
Han, Songmi ; Yang, Aerin ; Lee, Soonjang ; Lee, Han Woong ; Park, Chan Bae ; Park, Hee Sung. / Expanding the genetic code of Mus musculus. In: Nature Communications. 2017 ; Vol. 8.
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Han, S, Yang, A, Lee, S, Lee, HW, Park, CB & Park, HS 2017, 'Expanding the genetic code of Mus musculus', Nature Communications, vol. 8, 14568. https://doi.org/10.1038/ncomms14568

Expanding the genetic code of Mus musculus. / Han, Songmi; Yang, Aerin; Lee, Soonjang; Lee, Han Woong; Park, Chan Bae; Park, Hee Sung.

In: Nature Communications, Vol. 8, 14568, 21.02.2017.

Research output: Contribution to journalArticle

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