Background and aims: The pathogenesis of coronary artery disease (CAD) is closely associated with chronic inflammatory processes. CD8+ T cells are a key participant in the pathogenesis of atherosclerosis, the major cause of CAD; however, it remains unclear which CD8+ T-cell subset is responsible. We investigated the immunological features of CD8+ T cells expressing low and high levels of the IL-6 receptor α chain (IL-6Rα), a cytokine known to play a key role in cardiovascular diseases. Methods: The expression of IL-6Rα on CD8+ T cells and its association with plasma levels of soluble components of the IL-6/IL-6Rs as well as with clinical parameters were analyzed using FACS analysis and ELISA of CAD patients and age-matched healthy controls (HCs). Immunological characteristics of CD8+ T cells expressing low and high levels of IL-6Rα (CD8+IL-6Rαlow or high) were examined by in vitro culture and intracellular FACS analysis. Results: CAD patients had higher frequencies of circulating CD8+IL-6Rαlow effector memory (EM) T cells compared with HCs (median frequency; 74.59% vs. 60.09%, p = 0.0158). Expanded CD8+IL-6Rαlow T cells positively correlated with the frequency of senescent, cytotoxic CD8+CD57+ T cells (r = 0.6655, p < 0.0001) and plasma IL-6 level (r = 0.3995, p = 0.0432) in CAD patients. Loss of IL-6Rα expression on CD8+ T cells was induced by the combination of IL-6 and IL-15 with accompanying TCR-independent proliferation (p = 0.0101). Moreover, these CD8+IL-6Rαlow T cells had features of type 1 cytotoxic CD8+ T cells. Conclusions: Our findings suggest the possible involvement of expanded CD8+IL-6Rαlow EM T cells in CAD through their pro-inflammatory and highly cytotoxic capacities.
|Number of pages||8|
|Publication status||Published - 2016 Jun 1|
Bibliographical noteFunding Information:
This study was supported in part by a grant from the Korea Healthcare Technology R&D Project, Ministry for Health & Welfare Affairs, Republic of Korea ( HI13C0715 and HI13C0954 to W-W. L.) and a grant from the National Institutes of Health ( AG028069 to I.K.).
© 2016 Elsevier Ireland Ltd.
All Science Journal Classification (ASJC) codes
- Cardiology and Cardiovascular Medicine