Experimental leprosy in monkeys. II. Longitudinal serological observations in sooty mangabey monkeys

B. J. Gormus, K. Xu, S. N. Cho, G. B. Baskin, R. P. Bohm, L. N. Martin, J. L. Blanchard, P. A. Mack, M. S. Ratterree, W. M. Meyers, G. P. Walsh

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Abstract

In this study, 11 SMM were grouped and inoculated with differing doses of SMM-origin Mycobacterium leprae (ML) between 45 x 108 and 1 x 109 by either combined IV/IC routes or by IV or IC route alone. The combined route was the most effective in eliciting progressive, disseminated LL leprosy. In all, 6 of 7 SMM inoculated by the combined routes developed leprosy requiring treatment at some point. Only 1 of 4 inoculated by a single route developed persisting leprosy requiring chemotherapy. Either no disease or spontaneous regression of initial disease occurred in the other 3 animals inoculated by a single route. Doses in excess of 1 x 109 ML were more effective than lesser doses. An association was observed between the development of IgG anti-PGL-I ELISA OD values and resistance to leprosy and between IgM anti-PGL-I and leprosy progression or susceptibility. Serum PGL-I antigen levels, determined by dot ELISA, paralleled disease severity longitudinally. High positive OD values of anti-LAM IgG prior to ML inoculation were observed in the majority of leprosy susceptible SMM in contrast to negative levels in more resistant animals. Anti-LAM IgG OD values exceeded the positive cut-off point after inoculation in 5 of 11 SMM; 3 of these 5 had concurrent detectable serum levels of PGL-I antigen.

Original languageEnglish
Pages (from-to)105-125
Number of pages21
JournalLeprosy Review
Volume66
Issue number2
DOIs
Publication statusPublished - 1995

All Science Journal Classification (ASJC) codes

  • Dermatology
  • Infectious Diseases

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    Gormus, B. J., Xu, K., Cho, S. N., Baskin, G. B., Bohm, R. P., Martin, L. N., Blanchard, J. L., Mack, P. A., Ratterree, M. S., Meyers, W. M., & Walsh, G. P. (1995). Experimental leprosy in monkeys. II. Longitudinal serological observations in sooty mangabey monkeys. Leprosy Review, 66(2), 105-125. https://doi.org/10.5935/0305-7518.19950013