Exploiting antidiabetic activity of silver nanoparticles synthesized using punica granatum leaves and anticancer potential against human liver cancer cells (HepG2)

Rijuta G. Saratale, Han Seung Shin, Gopalakrishnan Kumar, Giovanni Benelli, Dong Su Kim, Ganesh D. Saratale

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

This study first time reports the novel synthesis of silver nanoparticles (AgNPs) using a Punica granatum leaf extract (PGE). The synthesized AgNPs were characterized by various analytical techniques including UV-Vis, Fourier transform infrared (FTIR), X-ray powder diffraction (XRD), X-ray photoelectron spectroscopy (XPS), field emission scanning electron microscopy and energy-dispersive spectra (FESEM-EDS) and high-resolution transmission electron microscopy (HRTEM). FTIR analysis revealed that the involvement of biological macromolecules of P. granatum leaf extract were distributed and involved in the synthesis and stabilization of AgNPs. A surface-sensitive technique of XPS was used to analyse the composition and oxidation state of synthesized AgNPs. The analytical results confirmed that the AgNPs were crystalline in nature with spherical shape. The zeta potential study revealed that the surface charge of synthesized AgNPs was highly negative (-26.6 mV) and particle size distribution was ranging from ~35 to 60nm and the average particle size was about 48nm determined by dynamic light scattering (DLS). The PGE-AgNPs antidiabetic potential exhibited effective inhibition against α-amylase and α-glucosidase (IC50; 65.2 and 53.8 lg/mL, respectively). The PGE-AgNPs showed a dose-dependent response against human liver cancer cells (HepG2) (IC50; 70lg/mL) indicating its greater efficacy in killing cancer cells. They also possessed in vitro free radical-scavenging activity in terms of ABTS (IC50; 52.2 μg/mL) and DPPH (IC50; 67.μg/mL) antioxidant activity. PGE-AgNPs displayed strong antibacterial activity and potent synergy with standard antibiotics against pathogenic bacteria. Thus, synthesized PGE-AgNPs show potential biomedical and industrial applications.

Original languageEnglish
Pages (from-to)211-222
Number of pages12
JournalArtificial Cells, Nanomedicine and Biotechnology
Volume46
Issue number1
DOIs
Publication statusPublished - 2018 Jun 13

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Punicaceae
Hep G2 Cells
Liver Neoplasms
Silver
Hypoglycemic Agents
Liver
Nanoparticles
Fourier transforms
X ray photoelectron spectroscopy
Cells
Infrared radiation
Enzyme inhibition
Amylases
Inhibitory Concentration 50
Scavenging
Antibiotics
Dynamic light scattering
Zeta potential
Surface charge
High resolution transmission electron microscopy

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Medicine (miscellaneous)
  • Biomedical Engineering
  • Pharmaceutical Science

Cite this

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title = "Exploiting antidiabetic activity of silver nanoparticles synthesized using punica granatum leaves and anticancer potential against human liver cancer cells (HepG2)",
abstract = "This study first time reports the novel synthesis of silver nanoparticles (AgNPs) using a Punica granatum leaf extract (PGE). The synthesized AgNPs were characterized by various analytical techniques including UV-Vis, Fourier transform infrared (FTIR), X-ray powder diffraction (XRD), X-ray photoelectron spectroscopy (XPS), field emission scanning electron microscopy and energy-dispersive spectra (FESEM-EDS) and high-resolution transmission electron microscopy (HRTEM). FTIR analysis revealed that the involvement of biological macromolecules of P. granatum leaf extract were distributed and involved in the synthesis and stabilization of AgNPs. A surface-sensitive technique of XPS was used to analyse the composition and oxidation state of synthesized AgNPs. The analytical results confirmed that the AgNPs were crystalline in nature with spherical shape. The zeta potential study revealed that the surface charge of synthesized AgNPs was highly negative (-26.6 mV) and particle size distribution was ranging from ~35 to 60nm and the average particle size was about 48nm determined by dynamic light scattering (DLS). The PGE-AgNPs antidiabetic potential exhibited effective inhibition against α-amylase and α-glucosidase (IC50; 65.2 and 53.8 lg/mL, respectively). The PGE-AgNPs showed a dose-dependent response against human liver cancer cells (HepG2) (IC50; 70lg/mL) indicating its greater efficacy in killing cancer cells. They also possessed in vitro free radical-scavenging activity in terms of ABTS (IC50; 52.2 μg/mL) and DPPH (IC50; 67.μg/mL) antioxidant activity. PGE-AgNPs displayed strong antibacterial activity and potent synergy with standard antibiotics against pathogenic bacteria. Thus, synthesized PGE-AgNPs show potential biomedical and industrial applications.",
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Exploiting antidiabetic activity of silver nanoparticles synthesized using punica granatum leaves and anticancer potential against human liver cancer cells (HepG2). / Saratale, Rijuta G.; Shin, Han Seung; Kumar, Gopalakrishnan; Benelli, Giovanni; Kim, Dong Su; Saratale, Ganesh D.

In: Artificial Cells, Nanomedicine and Biotechnology, Vol. 46, No. 1, 13.06.2018, p. 211-222.

Research output: Contribution to journalArticle

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