Exploiting fruit byproducts for eco-friendly nanosynthesis: Citrus × clementina peel extract mediated fabrication of silver nanoparticles with high efficacy against microbial pathogens and rat glial tumor C6 cells

Rijuta Ganesh Saratale, Han Seung Shin, Gopalakrishnan Kumar, Giovanni Benelli, Gajanan S. Ghodake, Yuan Yuan Jiang, Dong Su Kim, Ganesh Dattatraya Saratale

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Process byproducts from the fruit industry may represent a cheap and reliable source of green reducing agents to be used in current bio-nanosynthesis. This study reports the use of orange (Citrus × clementina) peel aqueous extract (OPE) for one-pot green synthesis of silver nanoparticles (AgNPs) with high effectiveness against various microbial pathogens as well as rat glial tumor C6 cells. The effects of various operational parameters on the synthesis of AgNPs were systematically investigated. The morphology, particle size, and properties of synthesized AgNPs were characterized using UV–visible spectroscopy, x-ray diffraction, x-ray photoelectron spectroscopy, field emission scanning electron microscopy, energy-dispersive x-ray spectroscopy, and Fourier transform infrared spectroscopy. High-resolution transmission electron microscopy shows that the nanoparticles are mostly spherical in shape and monodispersed, with an average particle size of 15–20 nm. Notably, the OPE-synthesized AgNPs were stable up to 6 months without change in their properties. Low doses of OPE-AgNPs inhibited the growth of human pathogens Escherichia coli, Bacillus cereus, and Staphylococcus aureus. The minimum inhibitory concentration and minimum bactericidal concentration of AgNPs against selected pathogenic bacteria were determined. OPE-AgNPs exhibited strong antioxidant activity in terms of ABTS (2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid)) radical scavenging (IC50 49.6 μg/mL) and DPPH (1,1-diphenyl-2-picrylhydrazyl) radical scavenging (IC50 63.4 μg/mL). OPE-AgNPs showed dose-dependent response against rat glial tumor C6 cells (LD50 60 μg/mL) showing a promising potential as anticancer agents. Overall, the current investigation highlighted a cheap green technology route to synthesize AgNPs using OPE byproducts and could potentially be utilized in biomedical, cosmetic, and pharmaceutical industry.

Original languageEnglish
Pages (from-to)10250-10263
Number of pages14
JournalEnvironmental Science and Pollution Research
Volume25
Issue number11
DOIs
Publication statusPublished - 2018 Apr 1

Fingerprint

Pathogens
Fruits
Silver
tumor
Glioma
Nanoparticles
Byproducts
Rats
Tumors
silver
Fruit
pathogen
fruit
spectroscopy
Scavenging
X-Rays
Particle Size
Fabrication
X rays
Inhibitory Concentration 50

All Science Journal Classification (ASJC) codes

  • Environmental Chemistry
  • Pollution
  • Health, Toxicology and Mutagenesis

Cite this

Saratale, Rijuta Ganesh ; Shin, Han Seung ; Kumar, Gopalakrishnan ; Benelli, Giovanni ; Ghodake, Gajanan S. ; Jiang, Yuan Yuan ; Kim, Dong Su ; Saratale, Ganesh Dattatraya. / Exploiting fruit byproducts for eco-friendly nanosynthesis : Citrus × clementina peel extract mediated fabrication of silver nanoparticles with high efficacy against microbial pathogens and rat glial tumor C6 cells. In: Environmental Science and Pollution Research. 2018 ; Vol. 25, No. 11. pp. 10250-10263.
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Exploiting fruit byproducts for eco-friendly nanosynthesis : Citrus × clementina peel extract mediated fabrication of silver nanoparticles with high efficacy against microbial pathogens and rat glial tumor C6 cells. / Saratale, Rijuta Ganesh; Shin, Han Seung; Kumar, Gopalakrishnan; Benelli, Giovanni; Ghodake, Gajanan S.; Jiang, Yuan Yuan; Kim, Dong Su; Saratale, Ganesh Dattatraya.

In: Environmental Science and Pollution Research, Vol. 25, No. 11, 01.04.2018, p. 10250-10263.

Research output: Contribution to journalArticle

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T2 - Citrus × clementina peel extract mediated fabrication of silver nanoparticles with high efficacy against microbial pathogens and rat glial tumor C6 cells

AU - Saratale, Rijuta Ganesh

AU - Shin, Han Seung

AU - Kumar, Gopalakrishnan

AU - Benelli, Giovanni

AU - Ghodake, Gajanan S.

AU - Jiang, Yuan Yuan

AU - Kim, Dong Su

AU - Saratale, Ganesh Dattatraya

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