Exploiting multimode waveguides for pure fibre based fluorescence imaging

Tomáš Čižár, Kishan Dholakia

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

There has been an immense drive in modern microscopy towards miniaturisation and fibre based technology. This has been necessitated by the need to access hostile or difficult environments particulalrly in-situ and in-vivo. Strategies to date have included the use of specialist fibres and miniaturised scanning systems accompanied by ingenious microfabricated lenses. In parallel recent studies of randomized light fields and their holographic control opened up new ways for imaging. We present a novel approach for this field by utilising disordered light within a standard multimode optical fibre for minimally invasive lenseless microscopy and optical mode conversion. We demonstrate scanning uorescence microscopy at acquisition rates allowing observation of dynamic processes such as Brownian motion of mesoscopic particles. As the sample plane can be defined at any distance from the fibre facet, we eliminate the need for complex or elaborate focusing optics (e.g. miniaturized objectives, GRIN lenses) and instead reconfigure the system dynamically to image different axial planes. Furthermore, we show how such control can realise a new form of mode converter and generate various types of advanced light fields such as propagation-invariant beams and optical vortices. These may be useful for future fibre based implementations of super-resolution or light sheet microscopy. To the best of our knowledge, this technology represents the narrowest possible image guiding system based on light propagation.

Original languageEnglish
Title of host publicationAdvanced Biomedical and Clinical Diagnostic Systems XI
DOIs
Publication statusPublished - 2013
EventAdvanced Biomedical and Clinical Diagnostic Systems XI - San Francisco, CA, United States
Duration: 2013 Feb 32013 Feb 5

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume8572
ISSN (Print)1605-7422

Conference

ConferenceAdvanced Biomedical and Clinical Diagnostic Systems XI
CountryUnited States
CitySan Francisco, CA
Period13/2/313/2/5

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging

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