TY - GEN
T1 - Exploiting multimode waveguides for pure fibre based fluorescence imaging
AU - Čižár, Tomáš
AU - Dholakia, Kishan
PY - 2013
Y1 - 2013
N2 - There has been an immense drive in modern microscopy towards miniaturisation and fibre based technology. This has been necessitated by the need to access hostile or difficult environments particulalrly in-situ and in-vivo. Strategies to date have included the use of specialist fibres and miniaturised scanning systems accompanied by ingenious microfabricated lenses. In parallel recent studies of randomized light fields and their holographic control opened up new ways for imaging. We present a novel approach for this field by utilising disordered light within a standard multimode optical fibre for minimally invasive lenseless microscopy and optical mode conversion. We demonstrate scanning uorescence microscopy at acquisition rates allowing observation of dynamic processes such as Brownian motion of mesoscopic particles. As the sample plane can be defined at any distance from the fibre facet, we eliminate the need for complex or elaborate focusing optics (e.g. miniaturized objectives, GRIN lenses) and instead reconfigure the system dynamically to image different axial planes. Furthermore, we show how such control can realise a new form of mode converter and generate various types of advanced light fields such as propagation-invariant beams and optical vortices. These may be useful for future fibre based implementations of super-resolution or light sheet microscopy. To the best of our knowledge, this technology represents the narrowest possible image guiding system based on light propagation.
AB - There has been an immense drive in modern microscopy towards miniaturisation and fibre based technology. This has been necessitated by the need to access hostile or difficult environments particulalrly in-situ and in-vivo. Strategies to date have included the use of specialist fibres and miniaturised scanning systems accompanied by ingenious microfabricated lenses. In parallel recent studies of randomized light fields and their holographic control opened up new ways for imaging. We present a novel approach for this field by utilising disordered light within a standard multimode optical fibre for minimally invasive lenseless microscopy and optical mode conversion. We demonstrate scanning uorescence microscopy at acquisition rates allowing observation of dynamic processes such as Brownian motion of mesoscopic particles. As the sample plane can be defined at any distance from the fibre facet, we eliminate the need for complex or elaborate focusing optics (e.g. miniaturized objectives, GRIN lenses) and instead reconfigure the system dynamically to image different axial planes. Furthermore, we show how such control can realise a new form of mode converter and generate various types of advanced light fields such as propagation-invariant beams and optical vortices. These may be useful for future fibre based implementations of super-resolution or light sheet microscopy. To the best of our knowledge, this technology represents the narrowest possible image guiding system based on light propagation.
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U2 - 10.1117/12.2003798
DO - 10.1117/12.2003798
M3 - Conference contribution
AN - SCOPUS:84877844125
SN - 9780819493415
T3 - Progress in Biomedical Optics and Imaging - Proceedings of SPIE
BT - Advanced Biomedical and Clinical Diagnostic Systems XI
T2 - Advanced Biomedical and Clinical Diagnostic Systems XI
Y2 - 3 February 2013 through 5 February 2013
ER -