Expression and activity of L-Myc in normal mouse development

Kimi S. Hatton, Kathleen Mahon, Lynda Chin, Fung Chow Chiu, Han Woong Lee, Damin Peng, Sharon D. Morgenbesser, James Horner, Ronald A. DePinho

Research output: Contribution to journalArticle

73 Citations (Scopus)

Abstract

To determine the role of L-Myc in normal mammalian development and its functional relationship to other members of the Myc family, we determined the normal patterns of L-myc gene expression in the developing mouse by RNA in situ hybridization and assessed the phenotypic impact of L-Myc deficiency produced through standard gene targeting methodology. L-myc transcripts were detected in the developing kidney and lung as well as in both the proliferative and the differentiative zones of the brain and neural tube. Despite significant expression of L-myc in developing mouse tissues, homozygous null L-myc mice were found to be viable, reproductively competent, and represented in expected frequencies from heterozygous matings. A detailed histological survey of embryonic and adult tissues, characterization of an embryonic neuronal marker, and measurement of cellular proliferation in situ did not reveal any congenital abnormalities. The lack of an apparent phenotype associated with L-Myc deficiency indicates that L-Myc is dispensable fur gross morphological development and argues against a unique role for L-Myc in early central nervous system development as had been previously suggested. Although overlapping expression patterns among myc family members raise the possibility of complementation of L-Myc deficiency by other Myc oncoproteins, compensatory changes in the levels of c- and/or N- myc transcripts were not detected in homozygous null L-myc mice.

Original languageEnglish
Pages (from-to)1794-1804
Number of pages11
JournalMolecular and cellular biology
Volume16
Issue number4
DOIs
Publication statusPublished - 1996 Apr

    Fingerprint

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Cell Biology

Cite this

Hatton, K. S., Mahon, K., Chin, L., Chiu, F. C., Lee, H. W., Peng, D., Morgenbesser, S. D., Horner, J., & DePinho, R. A. (1996). Expression and activity of L-Myc in normal mouse development. Molecular and cellular biology, 16(4), 1794-1804. https://doi.org/10.1128/MCB.16.4.1794