Expression and distribution of extracellular matrices during corneal wound healing after keratomileusis

S. I. Kang, E. K. Kim, H. Y. Kim, H. B. Kim

Research output: Contribution to journalArticle

Abstract

Purpose. To investigate the wound healing response following keratomileusis in situ in rabbits. Methods. Using immunofluorescence techniques, we studied the time course of the appearance and distribution of fibronectin, fibrin, and collagen type III at follow-up intervals from 1 hour to 3 months after keratomileusis in situ. Results. Three hours after induced injury, fibronectin and fibrin began to deposit from the periphery of denuded stromal surface, and formed continuous layer by 6 hours, progressively diminishing thereafter. Once the wound was reepithelialized, both fibronectin and fibrin disappeared. For keratomileusis in situ, collagen type III was detected only in the periphery of the cap-bed interface, where the collagen lamellae were cut in cross-section. The most striking finding was that in the central zone of the cap-bed interface where the cap was resected in parallel with the stromal collagen lamella, collagen type III was not detected. Conclusions After keratomileusis in situ in rabbits, fibronectin and fibrin play an important role in epithelial healing. By 7 days, collagen type III takes part in stromal healing, which probably takes place in the periphery. No deposition of this new collagen in the center of cap-bed interface may explain why optical clarity persists after keratomileusis in situ.

Original languageEnglish
Pages (from-to)S64
JournalInvestigative Ophthalmology and Visual Science
Volume37
Issue number3
Publication statusPublished - 1996 Feb 15

Fingerprint

Collagen Type III
Fibrin
Fibronectins
Wound Healing
Extracellular Matrix
Collagen
Rabbits
Wounds and Injuries
Fluorescent Antibody Technique

All Science Journal Classification (ASJC) codes

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

Cite this

@article{8527c0da3a584477a1a49d50c06205a7,
title = "Expression and distribution of extracellular matrices during corneal wound healing after keratomileusis",
abstract = "Purpose. To investigate the wound healing response following keratomileusis in situ in rabbits. Methods. Using immunofluorescence techniques, we studied the time course of the appearance and distribution of fibronectin, fibrin, and collagen type III at follow-up intervals from 1 hour to 3 months after keratomileusis in situ. Results. Three hours after induced injury, fibronectin and fibrin began to deposit from the periphery of denuded stromal surface, and formed continuous layer by 6 hours, progressively diminishing thereafter. Once the wound was reepithelialized, both fibronectin and fibrin disappeared. For keratomileusis in situ, collagen type III was detected only in the periphery of the cap-bed interface, where the collagen lamellae were cut in cross-section. The most striking finding was that in the central zone of the cap-bed interface where the cap was resected in parallel with the stromal collagen lamella, collagen type III was not detected. Conclusions After keratomileusis in situ in rabbits, fibronectin and fibrin play an important role in epithelial healing. By 7 days, collagen type III takes part in stromal healing, which probably takes place in the periphery. No deposition of this new collagen in the center of cap-bed interface may explain why optical clarity persists after keratomileusis in situ.",
author = "Kang, {S. I.} and Kim, {E. K.} and Kim, {H. Y.} and Kim, {H. B.}",
year = "1996",
month = "2",
day = "15",
language = "English",
volume = "37",
pages = "S64",
journal = "Investigative Ophthalmology and Visual Science",
issn = "0146-0404",
publisher = "Association for Research in Vision and Ophthalmology Inc.",
number = "3",

}

Expression and distribution of extracellular matrices during corneal wound healing after keratomileusis. / Kang, S. I.; Kim, E. K.; Kim, H. Y.; Kim, H. B.

In: Investigative Ophthalmology and Visual Science, Vol. 37, No. 3, 15.02.1996, p. S64.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Expression and distribution of extracellular matrices during corneal wound healing after keratomileusis

AU - Kang, S. I.

AU - Kim, E. K.

AU - Kim, H. Y.

AU - Kim, H. B.

PY - 1996/2/15

Y1 - 1996/2/15

N2 - Purpose. To investigate the wound healing response following keratomileusis in situ in rabbits. Methods. Using immunofluorescence techniques, we studied the time course of the appearance and distribution of fibronectin, fibrin, and collagen type III at follow-up intervals from 1 hour to 3 months after keratomileusis in situ. Results. Three hours after induced injury, fibronectin and fibrin began to deposit from the periphery of denuded stromal surface, and formed continuous layer by 6 hours, progressively diminishing thereafter. Once the wound was reepithelialized, both fibronectin and fibrin disappeared. For keratomileusis in situ, collagen type III was detected only in the periphery of the cap-bed interface, where the collagen lamellae were cut in cross-section. The most striking finding was that in the central zone of the cap-bed interface where the cap was resected in parallel with the stromal collagen lamella, collagen type III was not detected. Conclusions After keratomileusis in situ in rabbits, fibronectin and fibrin play an important role in epithelial healing. By 7 days, collagen type III takes part in stromal healing, which probably takes place in the periphery. No deposition of this new collagen in the center of cap-bed interface may explain why optical clarity persists after keratomileusis in situ.

AB - Purpose. To investigate the wound healing response following keratomileusis in situ in rabbits. Methods. Using immunofluorescence techniques, we studied the time course of the appearance and distribution of fibronectin, fibrin, and collagen type III at follow-up intervals from 1 hour to 3 months after keratomileusis in situ. Results. Three hours after induced injury, fibronectin and fibrin began to deposit from the periphery of denuded stromal surface, and formed continuous layer by 6 hours, progressively diminishing thereafter. Once the wound was reepithelialized, both fibronectin and fibrin disappeared. For keratomileusis in situ, collagen type III was detected only in the periphery of the cap-bed interface, where the collagen lamellae were cut in cross-section. The most striking finding was that in the central zone of the cap-bed interface where the cap was resected in parallel with the stromal collagen lamella, collagen type III was not detected. Conclusions After keratomileusis in situ in rabbits, fibronectin and fibrin play an important role in epithelial healing. By 7 days, collagen type III takes part in stromal healing, which probably takes place in the periphery. No deposition of this new collagen in the center of cap-bed interface may explain why optical clarity persists after keratomileusis in situ.

UR - http://www.scopus.com/inward/record.url?scp=33750150885&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33750150885&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:33750150885

VL - 37

SP - S64

JO - Investigative Ophthalmology and Visual Science

JF - Investigative Ophthalmology and Visual Science

SN - 0146-0404

IS - 3

ER -