Expression and functional role of formyl peptide receptor in human bone marrow-derived mesenchymal stem cells

Mi Kyoung Kim, Do Sik Min, Yoon Jeong Park, Jae Ho Kim, Sung Ho Ryu, Yoe Sik Bae

Research output: Contribution to journalArticlepeer-review

23 Citations (Scopus)

Abstract

We investigated the expression of formyl peptide receptor (FPR) and its functional role in human bone marrow-derived mesenchymal stem cells (MSCs). We analyzed the expression of FPR by using ligand-binding assay with radio-labeled N-formyl-met-leu-phe (fMLF), and found that MSCs express FPR. FMLF stimulated intracellular calcium increase, mitogen-activated protein kinases activation, and Akt activation, which were mediated by Gi proteins. MSCs were chemotactically migrated to fMLF. FMLF-induced MSC chemotaxis was also completely inhibited by pertussis toxin, LY294002, and PD98059, indicating the role of Gi proteins, phosphoinositide 3-kinase, and extracellular signal regulated protein kinase. N-terminal fragment of annexin-1, Anx-1(2-26), an endogenous agonist for FPR, also induced chemotactic migration of MSCs. Thus MSCs express functional FPR, suggesting a new (patho)physiological role of FPR and its ligands in regulating MSC trafficking during induction of injured tissue repair.

Original languageEnglish
Pages (from-to)1917-1922
Number of pages6
JournalFEBS Letters
Volume581
Issue number9
DOIs
Publication statusPublished - 2007 May 1

Bibliographical note

Funding Information:
This work was supported by a grant 02-PJ2-PG1-CH16-0002 from the Korea Health 21 R&D Project, Ministry of Health & Welfare, Republic of Korea, the Korea Science and Engineering Foundation through the Medical Science and Engineering Research Center for Cancer Molecular Therapy at Dong-A University, and by a grant of Biotechnology Development Program (Grant Number 2005-00115) from Ministry of Science and Technology (MOST), Republic of Korea.

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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