Expression and functional role of formyl peptide receptor in human bone marrow-derived mesenchymal stem cells

Mi Kyoung Kim, Do Sik Min, Yoon Jeong Park, Jae Ho Kim, Sung Ho Ryu, Yoe Sik Bae

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

We investigated the expression of formyl peptide receptor (FPR) and its functional role in human bone marrow-derived mesenchymal stem cells (MSCs). We analyzed the expression of FPR by using ligand-binding assay with radio-labeled N-formyl-met-leu-phe (fMLF), and found that MSCs express FPR. FMLF stimulated intracellular calcium increase, mitogen-activated protein kinases activation, and Akt activation, which were mediated by Gi proteins. MSCs were chemotactically migrated to fMLF. FMLF-induced MSC chemotaxis was also completely inhibited by pertussis toxin, LY294002, and PD98059, indicating the role of Gi proteins, phosphoinositide 3-kinase, and extracellular signal regulated protein kinase. N-terminal fragment of annexin-1, Anx-1(2-26), an endogenous agonist for FPR, also induced chemotactic migration of MSCs. Thus MSCs express functional FPR, suggesting a new (patho)physiological role of FPR and its ligands in regulating MSC trafficking during induction of injured tissue repair.

Original languageEnglish
Pages (from-to)1917-1922
Number of pages6
JournalFEBS Letters
Volume581
Issue number9
DOIs
Publication statusPublished - 2007 May 1

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Formyl Peptide Receptor
Stem cells
Mesenchymal Stromal Cells
Bone
Bone Marrow
Mitogen-Activated Protein Kinase 6
Chemical activation
Ligands
Annexins
2-(4-morpholinyl)-8-phenyl-4H-1-benzopyran-4-one
1-Phosphatidylinositol 4-Kinase
Extracellular Signal-Regulated MAP Kinases
Pertussis Toxin
Chemotaxis
Phosphatidylinositols
Mitogen-Activated Protein Kinases
Radio
Protein Kinases
Assays
Proteins

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Kim, Mi Kyoung ; Min, Do Sik ; Park, Yoon Jeong ; Kim, Jae Ho ; Ryu, Sung Ho ; Bae, Yoe Sik. / Expression and functional role of formyl peptide receptor in human bone marrow-derived mesenchymal stem cells. In: FEBS Letters. 2007 ; Vol. 581, No. 9. pp. 1917-1922.
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Expression and functional role of formyl peptide receptor in human bone marrow-derived mesenchymal stem cells. / Kim, Mi Kyoung; Min, Do Sik; Park, Yoon Jeong; Kim, Jae Ho; Ryu, Sung Ho; Bae, Yoe Sik.

In: FEBS Letters, Vol. 581, No. 9, 01.05.2007, p. 1917-1922.

Research output: Contribution to journalArticle

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