Expression and localization of surfactant proteins in human nasal epithelium

Kook Kim Jin, Sung Shik Kim, Won Rha Keung, Chang-Hoon Kim, Hoon Cho Jae, Chang Hoon Lee, Jeung Gweon Lee, Joo Heon Yoon

Research output: Contribution to journalArticle

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Abstract

Surfactant proteins (SPs), designated SP-A, SP-B, SP-C, and SP-D, play an important role in surfactant metabolism and host defense mechanisms in the lung. This study investigates expression of the different SP types in human nasal mucosa and cultured normal human nasal epithelial (NHNE) cells and whether the expression of SP mRNA is influenced by the degree of mucociliary differentiation. RT-PCR was performed with mRNA from cultured NHNE cells and nasal mucosa. Immunohistochemical staining for SPs was performed on nasal mucosa specimens. Western blot analysis was performed on cell lysates from cultured NHNE cells. SP-A2, SP-B, and SP-D mRNAs were expressed in normal NHNE cells and human nasal mucosa. SPs were localized in ciliated cells of the surface epithelium and serous acini of the submucosal glands. SP-A, SP-B, and SP-D proteins were expressed in cultured NHNE cells. The degree of mucociliary differentiation influenced expression of the SP gene. We demonstrate that SP-A, SP-B, and SP-D are expressed in human nasal mucosa and cultured NHNE cells. Further study of the functional role of SPs in the upper airway is required.

Original languageEnglish
JournalAmerican Journal of Physiology - Lung Cellular and Molecular Physiology
Volume292
Issue number4
DOIs
Publication statusPublished - 2007 Apr 1

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Nasal Mucosa
Surface-Active Agents
Nose
Pulmonary Surfactant-Associated Protein D
Proteins
Epithelial Cells
Pulmonary Surfactant-Associated Protein A
Messenger RNA
Protein C
varespladib methyl
Cultured Cells

All Science Journal Classification (ASJC) codes

  • Physiology
  • Pulmonary and Respiratory Medicine
  • Physiology (medical)
  • Cell Biology

Cite this

Jin, Kook Kim ; Kim, Sung Shik ; Keung, Won Rha ; Kim, Chang-Hoon ; Jae, Hoon Cho ; Lee, Chang Hoon ; Lee, Jeung Gweon ; Yoon, Joo Heon. / Expression and localization of surfactant proteins in human nasal epithelium. In: American Journal of Physiology - Lung Cellular and Molecular Physiology. 2007 ; Vol. 292, No. 4.
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Expression and localization of surfactant proteins in human nasal epithelium. / Jin, Kook Kim; Kim, Sung Shik; Keung, Won Rha; Kim, Chang-Hoon; Jae, Hoon Cho; Lee, Chang Hoon; Lee, Jeung Gweon; Yoon, Joo Heon.

In: American Journal of Physiology - Lung Cellular and Molecular Physiology, Vol. 292, No. 4, 01.04.2007.

Research output: Contribution to journalArticle

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