Expression and regulation of osteoprotegerin in adipose tissue

Juan Ji An, Dong He Han, Dol Mi Kim, Se Hwa Kim, Yumie Rhee, Eun Jig Lee, Sung Kil Lim

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

Purpose: Osteoprotegerin (OPG), a potent inhibitor of osteoclastic bone resorption, has a variety of biological functions that include anti-inflammatory effects. Adipocytes and osteoblasts share a common origin, and the formation of new blood vessels often precedes adipogenesis in developing adipose tissue microvasculature. We examined whether OPG is secreted from adipocytes, therefore contributing to the prevention of neovascularization and protecting the vessels from intimal inflammation and medial calcification. Materials and Methods: The mRNA expression of OPG and receptor activator of NF-κB ligand (RANKL) was measured in differentiated 3T3L1 adipocytes and adipose tissues. Results: OPG mRNA expression increased with the differentiation of 3T3L1 adipocytes, while RANKL expression was not significantly altered. OPG mRNA was expressed at higher levels in white adipose tissue than in brown adipose tissue and was most abundant in the epididymal portion. In differentiated 3T3L1 adipocytes, Rosiglitazone and insulin reduced the OPG/RANKL expression ratio in a dose- and time- dependent mariner. In contrast, tumor necrosis factor-α (TNF-a) increased the expression of both OPG and RANKL in a time-dependent manner. The OPG/RANKL ratio was at a maximum two hours after TNF-α treatment and then returned to control levels. Furthermore, OPG was abundantly secreted into the media after transfection of OPG cDNA with Phi C31 integrase into 3T3L1 cells. Conclusion: Our results indicate that OPG mRNA is expressed and regulated in the adipose tissue. Considering the rule of OPG in obesity-associated inflammatory changes in adipose tissue and vessels, we speculate that OPG may have both a protective function against inflammation and anti-angiogenic effects on adipose tissue.

Original languageEnglish
Pages (from-to)765-772
Number of pages8
JournalYonsei medical journal
Volume48
Issue number5
DOIs
Publication statusPublished - 2007 Oct 1

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Osteoprotegerin
Adipose Tissue
Adipocytes
Messenger RNA
rosiglitazone
Tunica Intima
Bone Density Conservation Agents
Inflammation
Integrases
Adipogenesis
White Adipose Tissue
Brown Adipose Tissue
Microvessels
Osteoblasts
Transfection
Blood Vessels

All Science Journal Classification (ASJC) codes

  • Medicine(all)

Cite this

An, Juan Ji ; Han, Dong He ; Kim, Dol Mi ; Kim, Se Hwa ; Rhee, Yumie ; Lee, Eun Jig ; Lim, Sung Kil. / Expression and regulation of osteoprotegerin in adipose tissue. In: Yonsei medical journal. 2007 ; Vol. 48, No. 5. pp. 765-772.
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abstract = "Purpose: Osteoprotegerin (OPG), a potent inhibitor of osteoclastic bone resorption, has a variety of biological functions that include anti-inflammatory effects. Adipocytes and osteoblasts share a common origin, and the formation of new blood vessels often precedes adipogenesis in developing adipose tissue microvasculature. We examined whether OPG is secreted from adipocytes, therefore contributing to the prevention of neovascularization and protecting the vessels from intimal inflammation and medial calcification. Materials and Methods: The mRNA expression of OPG and receptor activator of NF-κB ligand (RANKL) was measured in differentiated 3T3L1 adipocytes and adipose tissues. Results: OPG mRNA expression increased with the differentiation of 3T3L1 adipocytes, while RANKL expression was not significantly altered. OPG mRNA was expressed at higher levels in white adipose tissue than in brown adipose tissue and was most abundant in the epididymal portion. In differentiated 3T3L1 adipocytes, Rosiglitazone and insulin reduced the OPG/RANKL expression ratio in a dose- and time- dependent mariner. In contrast, tumor necrosis factor-α (TNF-a) increased the expression of both OPG and RANKL in a time-dependent manner. The OPG/RANKL ratio was at a maximum two hours after TNF-α treatment and then returned to control levels. Furthermore, OPG was abundantly secreted into the media after transfection of OPG cDNA with Phi C31 integrase into 3T3L1 cells. Conclusion: Our results indicate that OPG mRNA is expressed and regulated in the adipose tissue. Considering the rule of OPG in obesity-associated inflammatory changes in adipose tissue and vessels, we speculate that OPG may have both a protective function against inflammation and anti-angiogenic effects on adipose tissue.",
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Expression and regulation of osteoprotegerin in adipose tissue. / An, Juan Ji; Han, Dong He; Kim, Dol Mi; Kim, Se Hwa; Rhee, Yumie; Lee, Eun Jig; Lim, Sung Kil.

In: Yonsei medical journal, Vol. 48, No. 5, 01.10.2007, p. 765-772.

Research output: Contribution to journalArticle

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T1 - Expression and regulation of osteoprotegerin in adipose tissue

AU - An, Juan Ji

AU - Han, Dong He

AU - Kim, Dol Mi

AU - Kim, Se Hwa

AU - Rhee, Yumie

AU - Lee, Eun Jig

AU - Lim, Sung Kil

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N2 - Purpose: Osteoprotegerin (OPG), a potent inhibitor of osteoclastic bone resorption, has a variety of biological functions that include anti-inflammatory effects. Adipocytes and osteoblasts share a common origin, and the formation of new blood vessels often precedes adipogenesis in developing adipose tissue microvasculature. We examined whether OPG is secreted from adipocytes, therefore contributing to the prevention of neovascularization and protecting the vessels from intimal inflammation and medial calcification. Materials and Methods: The mRNA expression of OPG and receptor activator of NF-κB ligand (RANKL) was measured in differentiated 3T3L1 adipocytes and adipose tissues. Results: OPG mRNA expression increased with the differentiation of 3T3L1 adipocytes, while RANKL expression was not significantly altered. OPG mRNA was expressed at higher levels in white adipose tissue than in brown adipose tissue and was most abundant in the epididymal portion. In differentiated 3T3L1 adipocytes, Rosiglitazone and insulin reduced the OPG/RANKL expression ratio in a dose- and time- dependent mariner. In contrast, tumor necrosis factor-α (TNF-a) increased the expression of both OPG and RANKL in a time-dependent manner. The OPG/RANKL ratio was at a maximum two hours after TNF-α treatment and then returned to control levels. Furthermore, OPG was abundantly secreted into the media after transfection of OPG cDNA with Phi C31 integrase into 3T3L1 cells. Conclusion: Our results indicate that OPG mRNA is expressed and regulated in the adipose tissue. Considering the rule of OPG in obesity-associated inflammatory changes in adipose tissue and vessels, we speculate that OPG may have both a protective function against inflammation and anti-angiogenic effects on adipose tissue.

AB - Purpose: Osteoprotegerin (OPG), a potent inhibitor of osteoclastic bone resorption, has a variety of biological functions that include anti-inflammatory effects. Adipocytes and osteoblasts share a common origin, and the formation of new blood vessels often precedes adipogenesis in developing adipose tissue microvasculature. We examined whether OPG is secreted from adipocytes, therefore contributing to the prevention of neovascularization and protecting the vessels from intimal inflammation and medial calcification. Materials and Methods: The mRNA expression of OPG and receptor activator of NF-κB ligand (RANKL) was measured in differentiated 3T3L1 adipocytes and adipose tissues. Results: OPG mRNA expression increased with the differentiation of 3T3L1 adipocytes, while RANKL expression was not significantly altered. OPG mRNA was expressed at higher levels in white adipose tissue than in brown adipose tissue and was most abundant in the epididymal portion. In differentiated 3T3L1 adipocytes, Rosiglitazone and insulin reduced the OPG/RANKL expression ratio in a dose- and time- dependent mariner. In contrast, tumor necrosis factor-α (TNF-a) increased the expression of both OPG and RANKL in a time-dependent manner. The OPG/RANKL ratio was at a maximum two hours after TNF-α treatment and then returned to control levels. Furthermore, OPG was abundantly secreted into the media after transfection of OPG cDNA with Phi C31 integrase into 3T3L1 cells. Conclusion: Our results indicate that OPG mRNA is expressed and regulated in the adipose tissue. Considering the rule of OPG in obesity-associated inflammatory changes in adipose tissue and vessels, we speculate that OPG may have both a protective function against inflammation and anti-angiogenic effects on adipose tissue.

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