Long noncoding RNAs (lncRNAs) are involved in developmental processes and diseases and function as critical regulators of a number of different cancer types. Previous research has revealed that lncRNAs affect cervical cancer development. Steroid receptor activator (SRA), an lncRNA, serves as a critical regulator of gynecologic cancer. However, the association between SRA expression and cervical cancer remains unclear. In the present study, the SRA expression levels in patients with cervical cancer were examined and the association between SRA expression and clinicopathological factors was determined. SRA expression was observed in cervical cancer tissues (n=100) and corresponding normal tissues (n=22) using reverse transcription-quantitative polymerase chain reaction, and its associations with clinical parameters and prognosis were analyzed. SRA expression was significantly greater in tissues from patients with cervical cancer compared with in control patients (P<0.001). Multivariate analysis revealed that high SRA expression was an independent prognostic factor of overall survival (hazard ratio=3.714, P=0.031). The present study additionally investigated the biofunctional consequences of SRA overexpression in vitro using Cell Counting kit-8, wound healing migration and Matrigel invasion assays. The results demonstrated that SRA overexpression enhanced cell proliferation, migration and invasion in vitro. Furthermore, SRA overexpression induced the epithelial-mesenchymal transition (EMT). Therefore, SRA may promote tumor aggressiveness through the upregulation of EMT-associated genes. These results indicated that SRA may represent a novel biomarker for predicting recurrence and prognosis and serve as a promising therapeutic target in cervical cancer.
Bibliographical noteFunding Information:
The present study was supported by the Korea Health Technology R&D Project through the Korea Health Industry Development Institute, funded by the Ministry of Health and Welfare, Republic of Korea (grant no. HI17C0321) and by the Basic Science Research Program through the National Research Foundation of Korea funded by the Ministry of Education, Science and Technology (grant nos. NRF-2015R1A2A2A01008162,
Cell lines and cell culture. The human cervical squamous carcinoma SiHa cells was obtained from the Korean Cell Line Bank (Seoul, Korea) and provided by the Korea Gynecologic Cancer Bank through the Bio and Medical Technology Development Program of the Minister of Science, Information and Communication Technology and Future Planning, Korea. A total of 293 cells were purchased from American Type Culture Collection (Manassas, VA, USA). SiHa and 293 cells were cultured in Dulbecco's modified Eagle's medium. All culture media were supplemented with 10% (v/v) fetal bovine serum and 1% penicillin/streptomycin, and cell lines were maintained at 37˚C in a humidified atmosphere of 5% CO2 and 95% air. Culture medium was replaced with fresh medium every 2-3 days, and cells that had been passaged less than 20 times were used in the experiments.
The present study was supported by the Korea Health Technology R&D Project through the Korea Health Industry Development Institute, funded by the Ministry of Health and Welfare, Republic of Korea (grant no. HI17C0321) and by the Basic Science Research Program through the National Research Foundation of Korea funded by the Ministry of Education, Science and Technology (grant nos. NRF-2015R1A2A2A01008162, NRF-2017R1D1A3B03032983 and NRF-2015 R1C1A2A01053516).
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All Science Journal Classification (ASJC) codes
- Cancer Research