Expression of Autophagy-Related Proteins in Hürthle Cell Neoplasm Is Different from That in Follicular Neoplasm

Yoon Jin Cha, Hye Min Kim, JaSeung Koo

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Purpose. We aimed to evaluate expression of autophagy-related proteins in Hürthle cell neoplasm (HCN) and follicular neoplasm (FN) and assess the clinical implications. Methods. 265 FNs (112 follicular carcinomas and 153 follicular adenomas) and 108 HCNs (27 Hürthle cell carcinomas and 81 Hürthle cell adenomas) were made into a tissue microarray. Immunohistochemical staining and Western blot for autophagy-related proteins (beclin-1, light chain (LC) 3A, LC3B, p62, and BNIP3) were performed, and the results were statistically analyzed. Results. A higher expression rate of beclin-1, LC3B, p62, and BNIP3 was found in HCN than in FN (P<0.001). The expression rate of beclin-1, LC3B, p62, and BNIP3 was the highest in HCCs followed by HCAs, FCs, and FAs in that order (P<0.001). HCCs were positive for the largest number of autophagy-related proteins followed by HCAs, FCs, and FAs (P<0.001), and most of the positive markers identified in HCCs were the high autophagy type (P<0.001), defined by positive staining for three or more of the five autophagy-related proteins. Conclusion. The autophagy-related proteins, beclin-1, LC3A, LC3B, p62, and BNIP3, were more frequently expressed in HCNs than in FNs, and HCCs showed the highest expression rate.

Original languageEnglish
Article number1372387
JournalDisease Markers
Volume2017
DOIs
Publication statusPublished - 2017 Jan 1

Fingerprint

Neoplasms
Proteins
Adenoma
Staining and Labeling
Carcinoma
Autophagy
Microarrays
Western Blotting
Cells
Autophagy-Related Proteins
Tissue
Light
Beclin-1

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics
  • Clinical Biochemistry
  • Biochemistry, medical

Cite this

@article{6a2d61f1d4c448d5a9d78d1f551225cc,
title = "Expression of Autophagy-Related Proteins in H{\"u}rthle Cell Neoplasm Is Different from That in Follicular Neoplasm",
abstract = "Purpose. We aimed to evaluate expression of autophagy-related proteins in H{\"u}rthle cell neoplasm (HCN) and follicular neoplasm (FN) and assess the clinical implications. Methods. 265 FNs (112 follicular carcinomas and 153 follicular adenomas) and 108 HCNs (27 H{\"u}rthle cell carcinomas and 81 H{\"u}rthle cell adenomas) were made into a tissue microarray. Immunohistochemical staining and Western blot for autophagy-related proteins (beclin-1, light chain (LC) 3A, LC3B, p62, and BNIP3) were performed, and the results were statistically analyzed. Results. A higher expression rate of beclin-1, LC3B, p62, and BNIP3 was found in HCN than in FN (P<0.001). The expression rate of beclin-1, LC3B, p62, and BNIP3 was the highest in HCCs followed by HCAs, FCs, and FAs in that order (P<0.001). HCCs were positive for the largest number of autophagy-related proteins followed by HCAs, FCs, and FAs (P<0.001), and most of the positive markers identified in HCCs were the high autophagy type (P<0.001), defined by positive staining for three or more of the five autophagy-related proteins. Conclusion. The autophagy-related proteins, beclin-1, LC3A, LC3B, p62, and BNIP3, were more frequently expressed in HCNs than in FNs, and HCCs showed the highest expression rate.",
author = "Cha, {Yoon Jin} and Kim, {Hye Min} and JaSeung Koo",
year = "2017",
month = "1",
day = "1",
doi = "10.1155/2017/1372387",
language = "English",
volume = "2017",
journal = "Disease Markers",
issn = "0278-0240",
publisher = "IOS Press",

}

Expression of Autophagy-Related Proteins in Hürthle Cell Neoplasm Is Different from That in Follicular Neoplasm. / Cha, Yoon Jin; Kim, Hye Min; Koo, JaSeung.

In: Disease Markers, Vol. 2017, 1372387, 01.01.2017.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Expression of Autophagy-Related Proteins in Hürthle Cell Neoplasm Is Different from That in Follicular Neoplasm

AU - Cha, Yoon Jin

AU - Kim, Hye Min

AU - Koo, JaSeung

PY - 2017/1/1

Y1 - 2017/1/1

N2 - Purpose. We aimed to evaluate expression of autophagy-related proteins in Hürthle cell neoplasm (HCN) and follicular neoplasm (FN) and assess the clinical implications. Methods. 265 FNs (112 follicular carcinomas and 153 follicular adenomas) and 108 HCNs (27 Hürthle cell carcinomas and 81 Hürthle cell adenomas) were made into a tissue microarray. Immunohistochemical staining and Western blot for autophagy-related proteins (beclin-1, light chain (LC) 3A, LC3B, p62, and BNIP3) were performed, and the results were statistically analyzed. Results. A higher expression rate of beclin-1, LC3B, p62, and BNIP3 was found in HCN than in FN (P<0.001). The expression rate of beclin-1, LC3B, p62, and BNIP3 was the highest in HCCs followed by HCAs, FCs, and FAs in that order (P<0.001). HCCs were positive for the largest number of autophagy-related proteins followed by HCAs, FCs, and FAs (P<0.001), and most of the positive markers identified in HCCs were the high autophagy type (P<0.001), defined by positive staining for three or more of the five autophagy-related proteins. Conclusion. The autophagy-related proteins, beclin-1, LC3A, LC3B, p62, and BNIP3, were more frequently expressed in HCNs than in FNs, and HCCs showed the highest expression rate.

AB - Purpose. We aimed to evaluate expression of autophagy-related proteins in Hürthle cell neoplasm (HCN) and follicular neoplasm (FN) and assess the clinical implications. Methods. 265 FNs (112 follicular carcinomas and 153 follicular adenomas) and 108 HCNs (27 Hürthle cell carcinomas and 81 Hürthle cell adenomas) were made into a tissue microarray. Immunohistochemical staining and Western blot for autophagy-related proteins (beclin-1, light chain (LC) 3A, LC3B, p62, and BNIP3) were performed, and the results were statistically analyzed. Results. A higher expression rate of beclin-1, LC3B, p62, and BNIP3 was found in HCN than in FN (P<0.001). The expression rate of beclin-1, LC3B, p62, and BNIP3 was the highest in HCCs followed by HCAs, FCs, and FAs in that order (P<0.001). HCCs were positive for the largest number of autophagy-related proteins followed by HCAs, FCs, and FAs (P<0.001), and most of the positive markers identified in HCCs were the high autophagy type (P<0.001), defined by positive staining for three or more of the five autophagy-related proteins. Conclusion. The autophagy-related proteins, beclin-1, LC3A, LC3B, p62, and BNIP3, were more frequently expressed in HCNs than in FNs, and HCCs showed the highest expression rate.

UR - http://www.scopus.com/inward/record.url?scp=85027256818&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85027256818&partnerID=8YFLogxK

U2 - 10.1155/2017/1372387

DO - 10.1155/2017/1372387

M3 - Article

C2 - 28819333

AN - SCOPUS:85027256818

VL - 2017

JO - Disease Markers

JF - Disease Markers

SN - 0278-0240

M1 - 1372387

ER -