Expression of autotaxin (NPP-2) is closely linked to invasiveness of breast cancer cells

So Young Yang, Jangsoon Lee, Chang Gyo Park, Seonghwan Kim, Sungyoul Hong, Hyuncheol Chung, Seong Ki Min, Jeung Whan Han, Hyang Woo Lee, Hoi Young Lee

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Abstract

Autotaxin (ATX), originally isolated from human melanoma cells, is a novel metastasis-enhancing motogen and angiogenesis factor. In the present study, we compared the expression level of ATX mRNA between normal and breast cancer tissues and found that the expression of ATX mRNA was closely linked to invasiveness of cancer cells. Reverse transcriptase-polymerase chain reaction (RT-PCR) and immunohistochemical analysis showed higher cellular ATX mRNA expression in the cancer than normal breast tissues. MDA-MB-435S breast cancer cells, expressing higher amount of ATX mRNA, showed greater relative invasiveness to fibroblast-conditioned medium (FCM) than MCF7, MDA-MB-231, and HBL-100 breast cancer cells. Furthermore, ATX-transfected MCF7 cells showed increased motility and invasiveness than vector-transfected MCF7 cells. Collectively, our results suggest that the expression of ATX is closely linked to the invasiveness of breast cancer cells.

Original languageEnglish
Pages (from-to)603-608
Number of pages6
JournalClinical and Experimental Metastasis
Volume19
Issue number7
DOIs
Publication statusPublished - 2002 Dec 24

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All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

Cite this

Yang, S. Y., Lee, J., Park, C. G., Kim, S., Hong, S., Chung, H., Min, S. K., Han, J. W., Lee, H. W., & Lee, H. Y. (2002). Expression of autotaxin (NPP-2) is closely linked to invasiveness of breast cancer cells. Clinical and Experimental Metastasis, 19(7), 603-608. https://doi.org/10.1023/A:1020950420196