Expression of Ca2+-dependent synaptotagmin isoforms in mouse and rat parotid acinar cells

Hae Jo, Hae Mi Byun, Jong Hoon Kim, Min Seuk Kim, Seung Hyeoi Kim, Jeong Hee Hong, Jeong Taeg Seo, Syng Ill Lee, Dong Min Shin, Heung Kyu Son

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Abstract

Synaptotagmin is a Ca2+ sensing protein, which triggers a fusion of synaptic vesicles in neuronal transmission. Little is known regarding the expression of Ca2+-dependent synaptotagmin isoforms and their contribution to the release of secretory vesicles in mouse and rat parotid acinar cells. We investigated a type of Ca2+-dependent synaptotagmin and Ca2+ signaling in both rat and mouse parotid acinar cells using RT-PCR, micro-fluorometry, and amylase assay. Mouse parotid acinar cells exhibited much more sensitive amylase release in response to muscarinic stimulation than did rat parotid acinar cells. However, transient [Ca2+]i increases and Ca2+ influx in response to muscarinic stimulation in both cells were identical, suggesting that the expression or activity of the Ca2- sensing proteins is different. Seven Ca2+- dependent synaptotagmins, from 1 to 7, were expressed in the mouse parotid acinar cells. However, in the rat parotid acinar cells, only synaptotagmins 1, 3, 4 and 7 were expressed. These results indicate that the expression of Ca2+-dependent synaptotagmins may contribute to the release of secretory vesicles in parotid acinar cells.

Original languageEnglish
Pages (from-to)70-77
Number of pages8
JournalYonsei medical journal
Volume47
Issue number1
DOIs
Publication statusPublished - 2006 Feb 6

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All Science Journal Classification (ASJC) codes

  • Medicine(all)

Cite this

Jo, H., Byun, H. M., Kim, J. H., Kim, M. S., Kim, S. H., Hong, J. H., Seo, J. T., Lee, S. I., Shin, D. M., & Son, H. K. (2006). Expression of Ca2+-dependent synaptotagmin isoforms in mouse and rat parotid acinar cells. Yonsei medical journal, 47(1), 70-77. https://doi.org/10.3349/ymj.2006.47.1.70