Purpose. The purpose of this study was to investigate the expression of cancer-associated fibroblast- (CAF-) related proteins and the implications in breast phyllodes tumor (PT). Methods. Tissue microarrays of 194 PT cases (151 benign PT, 27 borderline PT, and 16 malignant PT) were constructed. We performed immunohistochemical staining for CAF-related proteins (podoplanin, prolyl 4-hydroxylase, FAP, S100A4, PDGFR /β, and NG2) and analyzed the results according to clinicopathologic parameters. Results. Expression of PDGFR and PDGFRβ in the stromal component increased with increasing histologic grade of PT (p=0.003 and p=0.034, resp.). Among clinicopathologic parameters, only expression of FAP in stroma was associated with distant metastasis (p=0.002). In univariate analysis, stromal expression of PDGFR was associated with shorter overall survival (p=0.002). In Cox multivariate analysis, stromal overgrowth and PDGFR stromal positivity were associated with shorter overall survival (p=0.006 and p=0.050, resp.). Furthermore, expression of PDGFRβ in stroma was associated with shorter overall survival in patients with malignant PT (p=0.041). Conclusion. Stromal expression of PDGFR and PDGFRβ increased with increasing histologic grade of PT. In addition, PDGFR stromal positivity was associated with shorter overall survival. These results suggest that CAFs are associated with breast PT progression.
Bibliographical noteFunding Information:
This study was supported by a grant from the National R&D Program for Cancer Control, Ministry of Health & Welfare, Republic of Korea (1420080). Additionally, it was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT and Future Planning (2015R1A1A1A05001209).
© 2016 Hye Min Kim et al.
All Science Journal Classification (ASJC) codes
- Molecular Biology
- Clinical Biochemistry
- Biochemistry, medical