The involvement of estrogen receptor beta (ERβ) in prostate carcinogenesis has been hypothesized. Several reports have shown that ERβ expression was decreased when prostate cells undergo neoplastic transformation, suggesting that it could play a tumor-suppressor role. By restoring ERβ expression in prostatic carcinoma cells by adenoviral delivery, we aimed to test this hypothesis. We observed that ERβ strongly inhibited the invasiveness and the growth of these cells. In addition, ERβ cells were undergoing apoptosis, as shown by quantification of Bax, poly(ADP-ribose) polymerase and caspase-3 expression. Our data suggest that ERβ acts as a tumor-suppressor by its anti-proliferative, anti-invasive and pro-apoptotic properties.
Bibliographical noteFunding Information:
We thank Professor B.S. Katzenellenbogen for the gift of ERβ antibody, ERα and ERβ cDNAs. We are also grateful to the Vector Core of the University Hospital of Nantes supported by the Association Française contre les Myopathies (AFM) for the production of Adenovirus. This work was supported by grants from ARC (Association pour la Recherche contre le Cancer, Grant No. 4302), la Ligue Nationale contre le Cancer (Comite du Gard), INSERM, and CNRS. This work was performed as partial fulfilment of the doctoral thesis of the primary author J. Cheng.
All Science Journal Classification (ASJC) codes
- Structural Biology
- Molecular Biology
- Cell Biology