Expression of laminin-5 with amniotic membrane transplantation in excimer laser ablated rat corneas

Jun Haeng Lee, Hyung Keun Lee, Jin Kuk Kim, Jee Ho Chang, Sung Eun Kim, Eung Kweon Kim, Gong Je Seong, Soonwon Hong

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3 Citations (Scopus)

Abstract

Purpose: To investigate the expression of laminin-5 during epithelial healing and evaluate its expression in vivo using rat corneas on which amniotic membrane was applied to cover the wound after excimer laser photoablation. Setting: Department of Ophthalmology, Yonsei University College of Medicine, Seoul, Korea. Methods: Myopic photorefractive keratectomy (PRK) with a 100 μm deep ablation was performed in Sprague Dawley rats killed 6, 12, 24, 48, 72, and 96 hours after the procedure. In the first group of 30 rats, the excimer laser-ablated cornea was covered with amniotic membrane after PRK. Thirty other rats in which no amniotic membrane treatment was used served as controls. Immunohistochemical and immunofluorescein techniques were used to monitor the expression of laminin-5, γ2, and γ1 in the rat corneas. Immunoblotting was used to compare the expression of laminin between the amniotic membrane group and the control group. Results: In the immunoblotting study, laminin-5, α3, and γ2 increased 24 hours after amniotic membrane treatment compared to the control group. At 12 hours, in vivo immunostaining of the corneas in both groups expressed laminin, but laminin-5 and γ2 were more intensely expressed in the amniotic membrane group. This continued until reepithelialization. Expression of the γ1 chain was not different between the 2 groups. Conclusion: With the use of amniotic membrane, the expression of laminin-5 and γ2 was faster and more intense than in a control group during reepithelialization of excimer laser-ablated rat corneas.

Original languageEnglish
Pages (from-to)2192-2199
Number of pages8
JournalJournal of Cataract and Refractive Surgery
Volume30
Issue number10
DOIs
Publication statusPublished - 2004 Oct 1

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Excimer Lasers
Amnion
Cornea
Transplantation
Photorefractive Keratectomy
Laminin
Immunoblotting
Control Groups
Ophthalmology
Korea
kalinin
Sprague Dawley Rats
Medicine
Wounds and Injuries
Therapeutics

All Science Journal Classification (ASJC) codes

  • Surgery
  • Ophthalmology
  • Sensory Systems

Cite this

Lee, Jun Haeng ; Lee, Hyung Keun ; Kim, Jin Kuk ; Chang, Jee Ho ; Kim, Sung Eun ; Kim, Eung Kweon ; Seong, Gong Je ; Hong, Soonwon. / Expression of laminin-5 with amniotic membrane transplantation in excimer laser ablated rat corneas. In: Journal of Cataract and Refractive Surgery. 2004 ; Vol. 30, No. 10. pp. 2192-2199.
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abstract = "Purpose: To investigate the expression of laminin-5 during epithelial healing and evaluate its expression in vivo using rat corneas on which amniotic membrane was applied to cover the wound after excimer laser photoablation. Setting: Department of Ophthalmology, Yonsei University College of Medicine, Seoul, Korea. Methods: Myopic photorefractive keratectomy (PRK) with a 100 μm deep ablation was performed in Sprague Dawley rats killed 6, 12, 24, 48, 72, and 96 hours after the procedure. In the first group of 30 rats, the excimer laser-ablated cornea was covered with amniotic membrane after PRK. Thirty other rats in which no amniotic membrane treatment was used served as controls. Immunohistochemical and immunofluorescein techniques were used to monitor the expression of laminin-5, γ2, and γ1 in the rat corneas. Immunoblotting was used to compare the expression of laminin between the amniotic membrane group and the control group. Results: In the immunoblotting study, laminin-5, α3, and γ2 increased 24 hours after amniotic membrane treatment compared to the control group. At 12 hours, in vivo immunostaining of the corneas in both groups expressed laminin, but laminin-5 and γ2 were more intensely expressed in the amniotic membrane group. This continued until reepithelialization. Expression of the γ1 chain was not different between the 2 groups. Conclusion: With the use of amniotic membrane, the expression of laminin-5 and γ2 was faster and more intense than in a control group during reepithelialization of excimer laser-ablated rat corneas.",
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Expression of laminin-5 with amniotic membrane transplantation in excimer laser ablated rat corneas. / Lee, Jun Haeng; Lee, Hyung Keun; Kim, Jin Kuk; Chang, Jee Ho; Kim, Sung Eun; Kim, Eung Kweon; Seong, Gong Je; Hong, Soonwon.

In: Journal of Cataract and Refractive Surgery, Vol. 30, No. 10, 01.10.2004, p. 2192-2199.

Research output: Contribution to journalArticle

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T1 - Expression of laminin-5 with amniotic membrane transplantation in excimer laser ablated rat corneas

AU - Lee, Jun Haeng

AU - Lee, Hyung Keun

AU - Kim, Jin Kuk

AU - Chang, Jee Ho

AU - Kim, Sung Eun

AU - Kim, Eung Kweon

AU - Seong, Gong Je

AU - Hong, Soonwon

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N2 - Purpose: To investigate the expression of laminin-5 during epithelial healing and evaluate its expression in vivo using rat corneas on which amniotic membrane was applied to cover the wound after excimer laser photoablation. Setting: Department of Ophthalmology, Yonsei University College of Medicine, Seoul, Korea. Methods: Myopic photorefractive keratectomy (PRK) with a 100 μm deep ablation was performed in Sprague Dawley rats killed 6, 12, 24, 48, 72, and 96 hours after the procedure. In the first group of 30 rats, the excimer laser-ablated cornea was covered with amniotic membrane after PRK. Thirty other rats in which no amniotic membrane treatment was used served as controls. Immunohistochemical and immunofluorescein techniques were used to monitor the expression of laminin-5, γ2, and γ1 in the rat corneas. Immunoblotting was used to compare the expression of laminin between the amniotic membrane group and the control group. Results: In the immunoblotting study, laminin-5, α3, and γ2 increased 24 hours after amniotic membrane treatment compared to the control group. At 12 hours, in vivo immunostaining of the corneas in both groups expressed laminin, but laminin-5 and γ2 were more intensely expressed in the amniotic membrane group. This continued until reepithelialization. Expression of the γ1 chain was not different between the 2 groups. Conclusion: With the use of amniotic membrane, the expression of laminin-5 and γ2 was faster and more intense than in a control group during reepithelialization of excimer laser-ablated rat corneas.

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