Expression of miRNAs in circulating exosomes derived from patients with persistent atrial fibrillation

Dasom Mun, Hyoeun Kim, Ji Young Kang, Hyelim Park, Hyewon Park, Seung Hyun Lee, Nuri Yun, Boyoung Joung

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Atrial fibrillation (AF), the most common type of cardiac arrhythmia, is thought to be regulated by changes in microRNA (miRNA) expression. However, the evidence for this is inconsistent. The high stability and expression of circulating exosomal miRNAs may allow their use as candidate biomarkers. For the discovery phase, exosomes were isolated from the serum of patients with supraventricular tachycardia (SVT) as the controls (n = 5) and with paroxysmal AF (n = 4) and persistent AF (n = 5) for microarray analysis of miRNAs. Forty-five miRNAs were expressed significantly higher (>1.5-fold) in patients with persistent AF, but not in patients with paroxysmal AF, relative to the levels in patients with SVT control. Notably, expression of 5 miRNAs (miRNA-103a, -107, -320d, -486, and let-7b) was elevated by more than 4.5-fold in patients with persistent AF. For the validation phase, miRNAs were analyzed using quantitative RT-PCR analysis in exosomes from the serum of patients with SVT control (n = 20) and patients with persistent AF (n = 40). These miRNAs and their target genes were involved in atrial function and structure, oxidative stress, and fibrosis pathways. These findings suggest that serum exosomal miRNAs might be used as novel biomarkers to reflect the progression of AF.-Mun, D., Kim, H., Kang, J.-Y., Park, H., Park, H., Lee, S.-H., Yun, N., Joung, B. Expression of miRNAs in circulating exosomes derived from patients with persistent atrial fibrillation.

Original languageEnglish
Pages (from-to)5979-5989
Number of pages11
JournalFASEB journal : official publication of the Federation of American Societies for Experimental Biology
Volume33
Issue number5
DOIs
Publication statusPublished - 2019 May 1

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Exosomes
MicroRNAs
Atrial Fibrillation
Supraventricular Tachycardia
Biomarkers
Atrial Function
Serum
Oxidative stress
Microarray Analysis
Microarrays
Cardiac Arrhythmias
Oxidative Stress
Fibrosis
Genes

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Genetics

Cite this

Mun, Dasom ; Kim, Hyoeun ; Kang, Ji Young ; Park, Hyelim ; Park, Hyewon ; Lee, Seung Hyun ; Yun, Nuri ; Joung, Boyoung. / Expression of miRNAs in circulating exosomes derived from patients with persistent atrial fibrillation. In: FASEB journal : official publication of the Federation of American Societies for Experimental Biology. 2019 ; Vol. 33, No. 5. pp. 5979-5989.
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Expression of miRNAs in circulating exosomes derived from patients with persistent atrial fibrillation. / Mun, Dasom; Kim, Hyoeun; Kang, Ji Young; Park, Hyelim; Park, Hyewon; Lee, Seung Hyun; Yun, Nuri; Joung, Boyoung.

In: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 33, No. 5, 01.05.2019, p. 5979-5989.

Research output: Contribution to journalArticle

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