Expression of MUC1 on corneal endothelium of human

Seung Eun Jung, KyoungYul Seo, Hyun Kim, Hyung Lae Kim, In Hyuk Chung, Eungkweon Kim

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Purpose. To determine the expression of MUC1 in the human corneal endothelium. Methods. Reverse transcription-polymerase chain reaction (RT-PCR) for MUC1 was performed with total RNA from endothelial cells extracted from the human cornea. In situ hybridization with sense and antisense probes of human MUC1 was performed on the human corneal endothelium, immunoblot analysis using monoclonal antibody specific for human MUC1 (HMFG-1, or VU4H5) was performed on collected human corneal endothelial cells, and immunohistochemistry on the human cornea, using the same antibodies. Results. MUC1 mRNA expression was observed by RT-PCR in the human corneal endothelium, and the nucleotide sequence from the amplified band was matched with known human MUC1. In situ hybridization studies showed the localization of MUC1 mRNA in the human corneal endothelium, and immunoblot assay demonstrated the presence of MUC1 protein (MW > 200 kd). In addition, MUC1 protein was observed on the apical surface of cells and at the superficial layer of the cytoplasm in immunohistochemical studies. Conclusions. Human corneal endothelial cells produce MUC1, which is known to have protective and lubricative roles.

Original languageEnglish
Pages (from-to)691-695
Number of pages5
JournalCornea
Volume21
Issue number7
DOIs
Publication statusPublished - 2002 Oct 1

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Corneal Endothelium
Endothelial Cells
Cornea
Reverse Transcription
In Situ Hybridization
Polymerase Chain Reaction
Messenger RNA
Cytoplasm
Proteins
Immunohistochemistry
Monoclonal Antibodies

All Science Journal Classification (ASJC) codes

  • Ophthalmology

Cite this

Jung, Seung Eun ; Seo, KyoungYul ; Kim, Hyun ; Kim, Hyung Lae ; Chung, In Hyuk ; Kim, Eungkweon. / Expression of MUC1 on corneal endothelium of human. In: Cornea. 2002 ; Vol. 21, No. 7. pp. 691-695.
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Expression of MUC1 on corneal endothelium of human. / Jung, Seung Eun; Seo, KyoungYul; Kim, Hyun; Kim, Hyung Lae; Chung, In Hyuk; Kim, Eungkweon.

In: Cornea, Vol. 21, No. 7, 01.10.2002, p. 691-695.

Research output: Contribution to journalArticle

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AU - Jung, Seung Eun

AU - Seo, KyoungYul

AU - Kim, Hyun

AU - Kim, Hyung Lae

AU - Chung, In Hyuk

AU - Kim, Eungkweon

PY - 2002/10/1

Y1 - 2002/10/1

N2 - Purpose. To determine the expression of MUC1 in the human corneal endothelium. Methods. Reverse transcription-polymerase chain reaction (RT-PCR) for MUC1 was performed with total RNA from endothelial cells extracted from the human cornea. In situ hybridization with sense and antisense probes of human MUC1 was performed on the human corneal endothelium, immunoblot analysis using monoclonal antibody specific for human MUC1 (HMFG-1, or VU4H5) was performed on collected human corneal endothelial cells, and immunohistochemistry on the human cornea, using the same antibodies. Results. MUC1 mRNA expression was observed by RT-PCR in the human corneal endothelium, and the nucleotide sequence from the amplified band was matched with known human MUC1. In situ hybridization studies showed the localization of MUC1 mRNA in the human corneal endothelium, and immunoblot assay demonstrated the presence of MUC1 protein (MW > 200 kd). In addition, MUC1 protein was observed on the apical surface of cells and at the superficial layer of the cytoplasm in immunohistochemical studies. Conclusions. Human corneal endothelial cells produce MUC1, which is known to have protective and lubricative roles.

AB - Purpose. To determine the expression of MUC1 in the human corneal endothelium. Methods. Reverse transcription-polymerase chain reaction (RT-PCR) for MUC1 was performed with total RNA from endothelial cells extracted from the human cornea. In situ hybridization with sense and antisense probes of human MUC1 was performed on the human corneal endothelium, immunoblot analysis using monoclonal antibody specific for human MUC1 (HMFG-1, or VU4H5) was performed on collected human corneal endothelial cells, and immunohistochemistry on the human cornea, using the same antibodies. Results. MUC1 mRNA expression was observed by RT-PCR in the human corneal endothelium, and the nucleotide sequence from the amplified band was matched with known human MUC1. In situ hybridization studies showed the localization of MUC1 mRNA in the human corneal endothelium, and immunoblot assay demonstrated the presence of MUC1 protein (MW > 200 kd). In addition, MUC1 protein was observed on the apical surface of cells and at the superficial layer of the cytoplasm in immunohistochemical studies. Conclusions. Human corneal endothelial cells produce MUC1, which is known to have protective and lubricative roles.

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