TY - JOUR
T1 - Expression of neurotrophic factors in human primary pterygeal tissue and selective TNF-α-induced stimulation of ciliary neurotrophic factor in pterygeal fibroblasts
AU - Hong, Samin
AU - Choi, Jin Young
AU - Lee, Hyung Keun
AU - Seong, Gong Je
AU - Seo, Kyoung Yul
AU - Kim, Eung Kweon
AU - Byeon, Suk Ho
PY - 2008/9/18
Y1 - 2008/9/18
N2 - Pterygium is a degenerative, fibrovascular and hyperplastic growth resulting from a proliferative tissue actively growing from altered limbal stem cells at the corneal-conjunctival junction. However, the pathogenesis of pterygium is still unclear. The aim of this study was to determine the expression of neurotrophic factors (nerve growth factor (NGF), brain-derived neurotrophic factor (BDNF), ciliary neurotrophic factor (CNTF), and neurotrophin (NT)-3, NT-4/5) in pterygeal tissue and to confirm whether tumor-necrosis factor-alpha (TNF-α) modulates the expression of these neurotrophic factors in primary cultured pterygeal fibroblasts. We looked for expression of these neurotrophic factors in surgically excised pterygia and normal donor limbal tissues by immunohistochemistry and in primary cultured pterygeal and normal subconjunctival Tenon's fibroblasts by Western blot and reverse transcription-polymerase chain reaction (RT-PCR). Expression of CNTF and NT-4/5 is increased in pterygeal tissues compared with normal tissue, and production of CNTF is stimulated in pterygeal fibroblasts by TNF-α but not in normal fibroblasts. This selective stimulation of CNTF in pterygeal fibroblasts suggests that CNTF may play a role in the pathogenesis of pterygium.
AB - Pterygium is a degenerative, fibrovascular and hyperplastic growth resulting from a proliferative tissue actively growing from altered limbal stem cells at the corneal-conjunctival junction. However, the pathogenesis of pterygium is still unclear. The aim of this study was to determine the expression of neurotrophic factors (nerve growth factor (NGF), brain-derived neurotrophic factor (BDNF), ciliary neurotrophic factor (CNTF), and neurotrophin (NT)-3, NT-4/5) in pterygeal tissue and to confirm whether tumor-necrosis factor-alpha (TNF-α) modulates the expression of these neurotrophic factors in primary cultured pterygeal fibroblasts. We looked for expression of these neurotrophic factors in surgically excised pterygia and normal donor limbal tissues by immunohistochemistry and in primary cultured pterygeal and normal subconjunctival Tenon's fibroblasts by Western blot and reverse transcription-polymerase chain reaction (RT-PCR). Expression of CNTF and NT-4/5 is increased in pterygeal tissues compared with normal tissue, and production of CNTF is stimulated in pterygeal fibroblasts by TNF-α but not in normal fibroblasts. This selective stimulation of CNTF in pterygeal fibroblasts suggests that CNTF may play a role in the pathogenesis of pterygium.
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U2 - 10.1016/j.etp.2008.04.013
DO - 10.1016/j.etp.2008.04.013
M3 - Article
C2 - 18585018
AN - SCOPUS:49949108334
SN - 0940-2993
VL - 60
SP - 513
EP - 520
JO - Experimentelle Pathologie
JF - Experimentelle Pathologie
IS - 6
ER -