Expression of phosphorylated extracellular signal-regulated kinase in rat kidneys exposed to high +Gz

Hyun Soo Kim, Youn Wha Kim

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Exposure to high gravitational acceleration forces acting along the body axis from the head to the feet (+Gz) severely reduces blood fl ow to the visceral organs, including the kidneys. Extracellular signal-regulated kinase (ERK) fi gures predominantly in mediating kidney cell responses to a wide variety of stress-related stimuli. Th ough previous studies have shown the activation of ERK in some experimental models, the regulation of ERK associated with +Gz exposure has not yet been investigated. Th e aim of this study was to examine the eff ect of high +Gz exposure on ERK activation in the kidneys. Using a small animal centrifuge, eight male Sprague-Dawley rats were exposed to +10Gz or +13Gz three times for 3 minutes each. Th e bilateral kidneys were obtained from each rat, and the expression levels of phosphorylated ERK (p-ERK) were evaluated using immunohistochemistry. In the control group, the collecting duct epithelium displayed faint cytoplasmic staining with no nuclear staining of p-ERK. By contrast, rats exposed to +10Gz showed strong nuclear staining intensity for p-ERK. In the renal papilla, the epithelial cells of collecting ducts and thin segments of the loop of Henle exhibited strong nuclear immunoreactivity for p-ERK. Rats exposed to +13Gz also showed the same staining intensity and distribution of p-ERK expression as that of rats exposed to +10Gz. Th is study is the fi rst to describe +Gz exposure-induced alteration in the expression of p-ERK in the kidneys. Our fi nding suggests that high +Gz exposure leads to the activation of ERK in the renal papilla.

Original languageEnglish
Pages (from-to)269-272
Number of pages4
JournalBosnian Journal of Basic Medical Sciences
Volume12
Issue number4
DOIs
Publication statusPublished - 2012 Jan 1

Fingerprint

Extracellular Signal-Regulated MAP Kinases
Kidney
Staining and Labeling
Loop of Henle
Sprague Dawley Rats
Foot
Theoretical Models
Epithelium
Epithelial Cells
Immunohistochemistry
Head
Control Groups

All Science Journal Classification (ASJC) codes

  • Medicine(all)

Cite this

@article{f7fa32e4a0b84b768444dedee85c3575,
title = "Expression of phosphorylated extracellular signal-regulated kinase in rat kidneys exposed to high +Gz",
abstract = "Exposure to high gravitational acceleration forces acting along the body axis from the head to the feet (+Gz) severely reduces blood fl ow to the visceral organs, including the kidneys. Extracellular signal-regulated kinase (ERK) fi gures predominantly in mediating kidney cell responses to a wide variety of stress-related stimuli. Th ough previous studies have shown the activation of ERK in some experimental models, the regulation of ERK associated with +Gz exposure has not yet been investigated. Th e aim of this study was to examine the eff ect of high +Gz exposure on ERK activation in the kidneys. Using a small animal centrifuge, eight male Sprague-Dawley rats were exposed to +10Gz or +13Gz three times for 3 minutes each. Th e bilateral kidneys were obtained from each rat, and the expression levels of phosphorylated ERK (p-ERK) were evaluated using immunohistochemistry. In the control group, the collecting duct epithelium displayed faint cytoplasmic staining with no nuclear staining of p-ERK. By contrast, rats exposed to +10Gz showed strong nuclear staining intensity for p-ERK. In the renal papilla, the epithelial cells of collecting ducts and thin segments of the loop of Henle exhibited strong nuclear immunoreactivity for p-ERK. Rats exposed to +13Gz also showed the same staining intensity and distribution of p-ERK expression as that of rats exposed to +10Gz. Th is study is the fi rst to describe +Gz exposure-induced alteration in the expression of p-ERK in the kidneys. Our fi nding suggests that high +Gz exposure leads to the activation of ERK in the renal papilla.",
author = "Kim, {Hyun Soo} and Kim, {Youn Wha}",
year = "2012",
month = "1",
day = "1",
doi = "10.17305/bjbms.2012.2453",
language = "English",
volume = "12",
pages = "269--272",
journal = "Bosnian Journal of Basic Medical Sciences",
issn = "1512-8601",
publisher = "Association of Basic Medical Sciences Federation of Bosnia and Herzegovina",
number = "4",

}

Expression of phosphorylated extracellular signal-regulated kinase in rat kidneys exposed to high +Gz. / Kim, Hyun Soo; Kim, Youn Wha.

In: Bosnian Journal of Basic Medical Sciences, Vol. 12, No. 4, 01.01.2012, p. 269-272.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Expression of phosphorylated extracellular signal-regulated kinase in rat kidneys exposed to high +Gz

AU - Kim, Hyun Soo

AU - Kim, Youn Wha

PY - 2012/1/1

Y1 - 2012/1/1

N2 - Exposure to high gravitational acceleration forces acting along the body axis from the head to the feet (+Gz) severely reduces blood fl ow to the visceral organs, including the kidneys. Extracellular signal-regulated kinase (ERK) fi gures predominantly in mediating kidney cell responses to a wide variety of stress-related stimuli. Th ough previous studies have shown the activation of ERK in some experimental models, the regulation of ERK associated with +Gz exposure has not yet been investigated. Th e aim of this study was to examine the eff ect of high +Gz exposure on ERK activation in the kidneys. Using a small animal centrifuge, eight male Sprague-Dawley rats were exposed to +10Gz or +13Gz three times for 3 minutes each. Th e bilateral kidneys were obtained from each rat, and the expression levels of phosphorylated ERK (p-ERK) were evaluated using immunohistochemistry. In the control group, the collecting duct epithelium displayed faint cytoplasmic staining with no nuclear staining of p-ERK. By contrast, rats exposed to +10Gz showed strong nuclear staining intensity for p-ERK. In the renal papilla, the epithelial cells of collecting ducts and thin segments of the loop of Henle exhibited strong nuclear immunoreactivity for p-ERK. Rats exposed to +13Gz also showed the same staining intensity and distribution of p-ERK expression as that of rats exposed to +10Gz. Th is study is the fi rst to describe +Gz exposure-induced alteration in the expression of p-ERK in the kidneys. Our fi nding suggests that high +Gz exposure leads to the activation of ERK in the renal papilla.

AB - Exposure to high gravitational acceleration forces acting along the body axis from the head to the feet (+Gz) severely reduces blood fl ow to the visceral organs, including the kidneys. Extracellular signal-regulated kinase (ERK) fi gures predominantly in mediating kidney cell responses to a wide variety of stress-related stimuli. Th ough previous studies have shown the activation of ERK in some experimental models, the regulation of ERK associated with +Gz exposure has not yet been investigated. Th e aim of this study was to examine the eff ect of high +Gz exposure on ERK activation in the kidneys. Using a small animal centrifuge, eight male Sprague-Dawley rats were exposed to +10Gz or +13Gz three times for 3 minutes each. Th e bilateral kidneys were obtained from each rat, and the expression levels of phosphorylated ERK (p-ERK) were evaluated using immunohistochemistry. In the control group, the collecting duct epithelium displayed faint cytoplasmic staining with no nuclear staining of p-ERK. By contrast, rats exposed to +10Gz showed strong nuclear staining intensity for p-ERK. In the renal papilla, the epithelial cells of collecting ducts and thin segments of the loop of Henle exhibited strong nuclear immunoreactivity for p-ERK. Rats exposed to +13Gz also showed the same staining intensity and distribution of p-ERK expression as that of rats exposed to +10Gz. Th is study is the fi rst to describe +Gz exposure-induced alteration in the expression of p-ERK in the kidneys. Our fi nding suggests that high +Gz exposure leads to the activation of ERK in the renal papilla.

UR - http://www.scopus.com/inward/record.url?scp=84871689043&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84871689043&partnerID=8YFLogxK

U2 - 10.17305/bjbms.2012.2453

DO - 10.17305/bjbms.2012.2453

M3 - Article

C2 - 23198944

AN - SCOPUS:84871689043

VL - 12

SP - 269

EP - 272

JO - Bosnian Journal of Basic Medical Sciences

JF - Bosnian Journal of Basic Medical Sciences

SN - 1512-8601

IS - 4

ER -