Expression of psoriasis-associated fatty acid-binding protein in senescent human dermal microvascular endothelial cells

Moon Kyung Ha, Kee Yang Chung, Ju Hee Lee, Dongsik Bang, Yoon Kee Park, Kwang Hoon Lee

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Aging is associated with the progressive pathophysiologic modification of endothelial cells. In vitro endothelial cell senescence is accompanied by proliferative activity failure and by perturbations in gene and protein expressions. Moreover, this cellular senescence in culture has been proposed to reflect processes that occur in aging organisms. In order to observe the changing patterns of protein expression in senescent human dermal microvascular endothelial cells (HDMECs), proteins obtained from both early- and late-passaged HDMECs were separated by two-dimensional electrophoresis, visualized by silver staining, and quantified by image processing. Proteins of interest were extracted by in-gel digestion with trypsin and quantified by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS), by searching the National Center for Biotechnology Information protein-sequence database. More than 2000 spots were detected by 2D electrophoresis within a linear pH range of 3-10. Twenty-two major differentially expressed spots were observed in serially passaged HDMECs and identified with high confidence by MALDI-TOF-MS. One of these spots was found to be a 14-15 kDa psoriasis-associated fatty acid-binding protein (PA-FABP) with high affinity for long-chain fatty acids. The expression of PA-FABP was confirmed to be elevated in senescent HDMECs (passage 20) by fluorescence-activated cell sorting (FACS), confocal laser microscopy, and by immunohistochemistry in aged human skin tissue. Our results suggest that the overexpression of FABP in cultured senescent HDMECs is closely related to skin aging.

Original languageEnglish
Pages (from-to)543-550
Number of pages8
JournalExperimental dermatology
Volume13
Issue number9
DOIs
Publication statusPublished - 2004 Sep 1

Fingerprint

Fatty Acid-Binding Proteins
Endothelial cells
Psoriasis
Endothelial Cells
Skin
Aging of materials
Cell Aging
Proteins
Electrophoresis
Confocal Microscopy
Ionization
Mass spectrometry
Lasers
Desorption
Mass Spectrometry
Skin Aging
Information Centers
Protein Databases
Silver Staining
Biotechnology

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Dermatology

Cite this

@article{9daad67de097472a8515256cdb332db1,
title = "Expression of psoriasis-associated fatty acid-binding protein in senescent human dermal microvascular endothelial cells",
abstract = "Aging is associated with the progressive pathophysiologic modification of endothelial cells. In vitro endothelial cell senescence is accompanied by proliferative activity failure and by perturbations in gene and protein expressions. Moreover, this cellular senescence in culture has been proposed to reflect processes that occur in aging organisms. In order to observe the changing patterns of protein expression in senescent human dermal microvascular endothelial cells (HDMECs), proteins obtained from both early- and late-passaged HDMECs were separated by two-dimensional electrophoresis, visualized by silver staining, and quantified by image processing. Proteins of interest were extracted by in-gel digestion with trypsin and quantified by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS), by searching the National Center for Biotechnology Information protein-sequence database. More than 2000 spots were detected by 2D electrophoresis within a linear pH range of 3-10. Twenty-two major differentially expressed spots were observed in serially passaged HDMECs and identified with high confidence by MALDI-TOF-MS. One of these spots was found to be a 14-15 kDa psoriasis-associated fatty acid-binding protein (PA-FABP) with high affinity for long-chain fatty acids. The expression of PA-FABP was confirmed to be elevated in senescent HDMECs (passage 20) by fluorescence-activated cell sorting (FACS), confocal laser microscopy, and by immunohistochemistry in aged human skin tissue. Our results suggest that the overexpression of FABP in cultured senescent HDMECs is closely related to skin aging.",
author = "Ha, {Moon Kyung} and Chung, {Kee Yang} and Lee, {Ju Hee} and Dongsik Bang and Park, {Yoon Kee} and Lee, {Kwang Hoon}",
year = "2004",
month = "9",
day = "1",
doi = "10.1111/j.0906-6705.2004.00196.x",
language = "English",
volume = "13",
pages = "543--550",
journal = "Experimental Dermatology",
issn = "0906-6705",
publisher = "Wiley-Blackwell",
number = "9",

}

Expression of psoriasis-associated fatty acid-binding protein in senescent human dermal microvascular endothelial cells. / Ha, Moon Kyung; Chung, Kee Yang; Lee, Ju Hee; Bang, Dongsik; Park, Yoon Kee; Lee, Kwang Hoon.

In: Experimental dermatology, Vol. 13, No. 9, 01.09.2004, p. 543-550.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Expression of psoriasis-associated fatty acid-binding protein in senescent human dermal microvascular endothelial cells

AU - Ha, Moon Kyung

AU - Chung, Kee Yang

AU - Lee, Ju Hee

AU - Bang, Dongsik

AU - Park, Yoon Kee

AU - Lee, Kwang Hoon

PY - 2004/9/1

Y1 - 2004/9/1

N2 - Aging is associated with the progressive pathophysiologic modification of endothelial cells. In vitro endothelial cell senescence is accompanied by proliferative activity failure and by perturbations in gene and protein expressions. Moreover, this cellular senescence in culture has been proposed to reflect processes that occur in aging organisms. In order to observe the changing patterns of protein expression in senescent human dermal microvascular endothelial cells (HDMECs), proteins obtained from both early- and late-passaged HDMECs were separated by two-dimensional electrophoresis, visualized by silver staining, and quantified by image processing. Proteins of interest were extracted by in-gel digestion with trypsin and quantified by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS), by searching the National Center for Biotechnology Information protein-sequence database. More than 2000 spots were detected by 2D electrophoresis within a linear pH range of 3-10. Twenty-two major differentially expressed spots were observed in serially passaged HDMECs and identified with high confidence by MALDI-TOF-MS. One of these spots was found to be a 14-15 kDa psoriasis-associated fatty acid-binding protein (PA-FABP) with high affinity for long-chain fatty acids. The expression of PA-FABP was confirmed to be elevated in senescent HDMECs (passage 20) by fluorescence-activated cell sorting (FACS), confocal laser microscopy, and by immunohistochemistry in aged human skin tissue. Our results suggest that the overexpression of FABP in cultured senescent HDMECs is closely related to skin aging.

AB - Aging is associated with the progressive pathophysiologic modification of endothelial cells. In vitro endothelial cell senescence is accompanied by proliferative activity failure and by perturbations in gene and protein expressions. Moreover, this cellular senescence in culture has been proposed to reflect processes that occur in aging organisms. In order to observe the changing patterns of protein expression in senescent human dermal microvascular endothelial cells (HDMECs), proteins obtained from both early- and late-passaged HDMECs were separated by two-dimensional electrophoresis, visualized by silver staining, and quantified by image processing. Proteins of interest were extracted by in-gel digestion with trypsin and quantified by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS), by searching the National Center for Biotechnology Information protein-sequence database. More than 2000 spots were detected by 2D electrophoresis within a linear pH range of 3-10. Twenty-two major differentially expressed spots were observed in serially passaged HDMECs and identified with high confidence by MALDI-TOF-MS. One of these spots was found to be a 14-15 kDa psoriasis-associated fatty acid-binding protein (PA-FABP) with high affinity for long-chain fatty acids. The expression of PA-FABP was confirmed to be elevated in senescent HDMECs (passage 20) by fluorescence-activated cell sorting (FACS), confocal laser microscopy, and by immunohistochemistry in aged human skin tissue. Our results suggest that the overexpression of FABP in cultured senescent HDMECs is closely related to skin aging.

UR - http://www.scopus.com/inward/record.url?scp=4544378288&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=4544378288&partnerID=8YFLogxK

U2 - 10.1111/j.0906-6705.2004.00196.x

DO - 10.1111/j.0906-6705.2004.00196.x

M3 - Article

C2 - 15335354

AN - SCOPUS:4544378288

VL - 13

SP - 543

EP - 550

JO - Experimental Dermatology

JF - Experimental Dermatology

SN - 0906-6705

IS - 9

ER -