Expression of the homotypic adhesion molecule E-cadherin by immature murine thymocytes and thymic epithelial cells

Min Geol Lee, Susan O. Sharrow, Andrew G. Farr, Alfred Singer, Mark C. Udey

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Abstract

Cadherins mediate homotypic adhesion between lineage-related cells in epithelia and other tissues. One cadherin, E-cadherin, is also responsible for adhesion of murine epidermal Langerhans cells to keratinocytes in vitro, and may play a role in the localization of Langerhans cells in epidermis. The thymus is another tissue in which important adhesive interactions between bone marrow-derived cells and keratinizing epithelia occur. To determine whether cadherins might be involved in interactions between thymocytes and thymic epithelial cells, we examined thymocytes from C57BL/6 mice of various gestational ages for cadherin expression. Most day 14 (D14) and essentially all D16 isolated fetal thymocytes expressed cell surface E-cadherin. After D16, the proportion of fetal thymocytes expressing E-cadherin and the level of E-cadherin expressed by individual thymocytes decreased with increasing gestational age. A minority of neonatal thymocytes and very few adult thymocytes expressed E-cadherin. E-cadherin was maximally expressed by the least mature (CD4-CD8-, HSA (J11d)(high), CD5 (Ly-1)(low), CD25 (IL- 2Rα)+) thymocytes. P-cadherin, another epithelial cadherin, was not detected on thymocytes at any stage of development. Immunohistologic studies revealed that thymic epithelial cells also expressed E-cadherin. Similar levels of E-cadherin were expressed by neonatal and adult thymic epithelial cells in situ, and E-cadherin was easily demonstrable on the thymic epithelial cell line, TE-71. In contrast, P-cadherin was transiently expressed by thymic epithelial cells in situ, and only small amounts of P- cadherin were detected on TE-71 cells. These studies demonstrate that thymocytes and thymic epithelial cells each have the capacity to express the homotypic adhesion molecule E-cadherin. E-cadherin may play a role in developmentally regulated interactions between early thymocytes and thymic stromal cells.

Original languageEnglish
Pages (from-to)5653-5659
Number of pages7
JournalJournal of Immunology
Volume152
Issue number12
Publication statusPublished - 1994 Jun 15

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Thymocytes
Cadherins
Epithelial Cells
Langerhans Cells
Gestational Age
Epithelium
Stromal Cells

All Science Journal Classification (ASJC) codes

  • Immunology

Cite this

Lee, Min Geol ; Sharrow, Susan O. ; Farr, Andrew G. ; Singer, Alfred ; Udey, Mark C. / Expression of the homotypic adhesion molecule E-cadherin by immature murine thymocytes and thymic epithelial cells. In: Journal of Immunology. 1994 ; Vol. 152, No. 12. pp. 5653-5659.
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abstract = "Cadherins mediate homotypic adhesion between lineage-related cells in epithelia and other tissues. One cadherin, E-cadherin, is also responsible for adhesion of murine epidermal Langerhans cells to keratinocytes in vitro, and may play a role in the localization of Langerhans cells in epidermis. The thymus is another tissue in which important adhesive interactions between bone marrow-derived cells and keratinizing epithelia occur. To determine whether cadherins might be involved in interactions between thymocytes and thymic epithelial cells, we examined thymocytes from C57BL/6 mice of various gestational ages for cadherin expression. Most day 14 (D14) and essentially all D16 isolated fetal thymocytes expressed cell surface E-cadherin. After D16, the proportion of fetal thymocytes expressing E-cadherin and the level of E-cadherin expressed by individual thymocytes decreased with increasing gestational age. A minority of neonatal thymocytes and very few adult thymocytes expressed E-cadherin. E-cadherin was maximally expressed by the least mature (CD4-CD8-, HSA (J11d)(high), CD5 (Ly-1)(low), CD25 (IL- 2Rα)+) thymocytes. P-cadherin, another epithelial cadherin, was not detected on thymocytes at any stage of development. Immunohistologic studies revealed that thymic epithelial cells also expressed E-cadherin. Similar levels of E-cadherin were expressed by neonatal and adult thymic epithelial cells in situ, and E-cadherin was easily demonstrable on the thymic epithelial cell line, TE-71. In contrast, P-cadherin was transiently expressed by thymic epithelial cells in situ, and only small amounts of P- cadherin were detected on TE-71 cells. These studies demonstrate that thymocytes and thymic epithelial cells each have the capacity to express the homotypic adhesion molecule E-cadherin. E-cadherin may play a role in developmentally regulated interactions between early thymocytes and thymic stromal cells.",
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Expression of the homotypic adhesion molecule E-cadherin by immature murine thymocytes and thymic epithelial cells. / Lee, Min Geol; Sharrow, Susan O.; Farr, Andrew G.; Singer, Alfred; Udey, Mark C.

In: Journal of Immunology, Vol. 152, No. 12, 15.06.1994, p. 5653-5659.

Research output: Contribution to journalArticle

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