Abstract
Background: Surgically induced necrotizing scleritis is primarily managed with immunosuppressive regimens. Such treatments can alter inflammatory cytokines and products such as tumor necrosis factor-α (TNF-α) and matrix metalloproteinase-9 (MMP-9), which are an important proinflammatory cytokine and a tissue-degrading protease involved in necrosis of some ocular tissues. Object: To evaluate TNF-α and MMP-9 in the tear or sclera of surgically induced necrotizing scleritis. Methods: After tear collection from healthy and diseased eyes of 2 patients, immunoblot analysis using monoclonal antibody specific for human TNF-α and MMP-9 was performed. In another patient, scleral tissues were obtained during scleral allograft surgery, and the same immunoblot analysis was done. Results: The level of TNF-α and MMP-9 was increased in tear fluid from patients compared to that of control volunteers and returned to the control level after treatment. The diseased sclera showed an increased expression of MMP-9 compared to that of the normal donor sclera. Conclusions: TNF-α and MMP-9 may suggest disease activity of surgically induced necrotizing scleritis and can be altered by proper immunosuppressive treatment.
| Original language | English |
|---|---|
| Pages (from-to) | 66-70 |
| Number of pages | 5 |
| Journal | Ophthalmic Research |
| Volume | 38 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 2006 Feb 1 |
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All Science Journal Classification (ASJC) codes
- Ophthalmology
- Sensory Systems
- Cellular and Molecular Neuroscience
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Expression of tumor necrosis factor alpha and matrix metalloproteinase-9 in surgically induced necrotizing scleritis. / Seo, Kyoung Yul; Lee, Hyung Keun; Kim, Eung Kweon; Lee, Joon H.
In: Ophthalmic Research, Vol. 38, No. 2, 01.02.2006, p. 66-70.Research output: Contribution to journal › Article
TY - JOUR
T1 - Expression of tumor necrosis factor alpha and matrix metalloproteinase-9 in surgically induced necrotizing scleritis
AU - Seo, Kyoung Yul
AU - Lee, Hyung Keun
AU - Kim, Eung Kweon
AU - Lee, Joon H.
PY - 2006/2/1
Y1 - 2006/2/1
N2 - Background: Surgically induced necrotizing scleritis is primarily managed with immunosuppressive regimens. Such treatments can alter inflammatory cytokines and products such as tumor necrosis factor-α (TNF-α) and matrix metalloproteinase-9 (MMP-9), which are an important proinflammatory cytokine and a tissue-degrading protease involved in necrosis of some ocular tissues. Object: To evaluate TNF-α and MMP-9 in the tear or sclera of surgically induced necrotizing scleritis. Methods: After tear collection from healthy and diseased eyes of 2 patients, immunoblot analysis using monoclonal antibody specific for human TNF-α and MMP-9 was performed. In another patient, scleral tissues were obtained during scleral allograft surgery, and the same immunoblot analysis was done. Results: The level of TNF-α and MMP-9 was increased in tear fluid from patients compared to that of control volunteers and returned to the control level after treatment. The diseased sclera showed an increased expression of MMP-9 compared to that of the normal donor sclera. Conclusions: TNF-α and MMP-9 may suggest disease activity of surgically induced necrotizing scleritis and can be altered by proper immunosuppressive treatment.
AB - Background: Surgically induced necrotizing scleritis is primarily managed with immunosuppressive regimens. Such treatments can alter inflammatory cytokines and products such as tumor necrosis factor-α (TNF-α) and matrix metalloproteinase-9 (MMP-9), which are an important proinflammatory cytokine and a tissue-degrading protease involved in necrosis of some ocular tissues. Object: To evaluate TNF-α and MMP-9 in the tear or sclera of surgically induced necrotizing scleritis. Methods: After tear collection from healthy and diseased eyes of 2 patients, immunoblot analysis using monoclonal antibody specific for human TNF-α and MMP-9 was performed. In another patient, scleral tissues were obtained during scleral allograft surgery, and the same immunoblot analysis was done. Results: The level of TNF-α and MMP-9 was increased in tear fluid from patients compared to that of control volunteers and returned to the control level after treatment. The diseased sclera showed an increased expression of MMP-9 compared to that of the normal donor sclera. Conclusions: TNF-α and MMP-9 may suggest disease activity of surgically induced necrotizing scleritis and can be altered by proper immunosuppressive treatment.
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UR - http://www.scopus.com/inward/citedby.url?scp=33644659336&partnerID=8YFLogxK
U2 - 10.1159/000090010
DO - 10.1159/000090010
M3 - Article
C2 - 16319517
AN - SCOPUS:33644659336
VL - 38
SP - 66
EP - 70
JO - Ophthalmic Research
JF - Ophthalmic Research
SN - 0030-3747
IS - 2
ER -