Expression patterns of ABCG2, Bmi-1, Oct-3/4, and Yap in the developing mouse incisor

Liwen Li, Hyuk Jae Kwon, Hidemitsu Harada, Hayato Ohshima, Sung Won Cho, Hansung Jung

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Abstract

Recent studies have demonstrated the existence of dental stem cells in the continuously growing tooth. However, much remains to be learned about the complex mechanism involving stem cells during tooth development. We determined the expression patterns of four stem cell markers ABCG2, Bmi-1, Oct-3/4, and Yap in the developing mouse incisors between embryonic day (E) 11 and postnatal day (PN) 20. ABCG2 was localized strongly in the perivascular region of the incisor mesenchyme from E11 to PN20, and in the odontoblasts from E18 to PN20. Bmi-1 was expressed in both the dental epithelium and mesenchyme from E11 to E14. The expression of Bmi-1 was noticeably reduced at E16, and was restricted to the apical bud from E16 to PN20. Oct-3/4 was localized in the nucleus of the cells in the superficial layer and stellate reticulum within the dental epithelium from E11 to E14 and in the apical bud from E16 to PN20. Meanwhile, once the ameloblasts and odontoblasts began to appear at E16, they expressed Oct-3/4 in the cytoplasm. Yap was expressed in most of the basal cells of the incisor dental epithelium from E11 to E14, but was expressed mainly in the transit-amplifying (TA) cells within the basal cell layer from E16 to PN20. The unique and overlapping expression patterns of ABCG2, Bmi-1, Oct-3/4, and Yap suggest the independent and interactive functions of the four stem cell markers in the developing mouse incisor.

Original languageEnglish
Pages (from-to)163-170
Number of pages8
JournalGene Expression Patterns
Volume11
Issue number3-4
DOIs
Publication statusPublished - 2011 Mar 1

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Incisor
Tooth
Stem Cells
Odontoblasts
Epithelium
Mesoderm
Ameloblasts
Reticulum
Cell Nucleus
Cytoplasm

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics
  • Developmental Biology

Cite this

Li, Liwen ; Kwon, Hyuk Jae ; Harada, Hidemitsu ; Ohshima, Hayato ; Cho, Sung Won ; Jung, Hansung. / Expression patterns of ABCG2, Bmi-1, Oct-3/4, and Yap in the developing mouse incisor. In: Gene Expression Patterns. 2011 ; Vol. 11, No. 3-4. pp. 163-170.
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Expression patterns of ABCG2, Bmi-1, Oct-3/4, and Yap in the developing mouse incisor. / Li, Liwen; Kwon, Hyuk Jae; Harada, Hidemitsu; Ohshima, Hayato; Cho, Sung Won; Jung, Hansung.

In: Gene Expression Patterns, Vol. 11, No. 3-4, 01.03.2011, p. 163-170.

Research output: Contribution to journalArticle

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