Expression patterns of aurora kinase B, heat shock protein 47, and periostin in esophageal squamous cell carcinoma

Yong Jae Kwon, Seog Joo Lee, Jae Soo Koh, Sung Han Kim, Young-Joon Kim, Jong Ho Park

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

To elucidate the proteins related to the development and progress of esophageal squamous cell carcinoma (ESCC), we performed gene expression profile analysis of 14 ESCC tissues. We identified 182 genes that were commonly upregulated (p < 10-5) and 54 genes that were downregulated in ESCC tissues (p < 10-6). In order to validate the gene expression profiles, we did semiquantitative RT-PCR analysis and found 11 genes upregulated in greater than 70% of 19 tissue samples. We further examined the protein expression level and the distribution patterns of four genes: aurora kinase B (AURKB), periostin (POSTN), heat shock protein 47 (HSP47), and matrix metalloprotease1 (MMP1). Ultimately, three genes (AURKB, HSP47, POSTN) were verified to be increased at both the mRNA and protein levels. Among them, AURKB and HSP47 were found to increase in the early development stage of ESCC and POSTN might be considered to function in the cell-cell interactions between cancer cell and adjacent stromal cells. Accordingly, these studies may provide valuable information for the identification of the genes that are necessary to study further their functions. Moreover, these genes might be used as potential diagnostic or therapeutic target molecules for ESCC patients.

Original languageEnglish
Pages (from-to)141-151
Number of pages11
JournalOncology Research
Volume18
Issue number4
DOIs
Publication statusPublished - 2009 Jan 1

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HSP47 Heat-Shock Proteins
Aurora Kinase B
Genes
Transcriptome
Proteins
Stromal Cells
Esophageal Squamous Cell Carcinoma
Cell Communication
Down-Regulation
Polymerase Chain Reaction
Messenger RNA

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

Cite this

Kwon, Yong Jae ; Lee, Seog Joo ; Koh, Jae Soo ; Kim, Sung Han ; Kim, Young-Joon ; Park, Jong Ho. / Expression patterns of aurora kinase B, heat shock protein 47, and periostin in esophageal squamous cell carcinoma. In: Oncology Research. 2009 ; Vol. 18, No. 4. pp. 141-151.
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Expression patterns of aurora kinase B, heat shock protein 47, and periostin in esophageal squamous cell carcinoma. / Kwon, Yong Jae; Lee, Seog Joo; Koh, Jae Soo; Kim, Sung Han; Kim, Young-Joon; Park, Jong Ho.

In: Oncology Research, Vol. 18, No. 4, 01.01.2009, p. 141-151.

Research output: Contribution to journalArticle

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