Expression patterns of the Fam83h gene during murine tooth development

Min Jung Lee, Sook Kyung Lee, Kyung Eun Lee, Hee Yun Kang, Hansung Jung, Jung Wook Kim

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Aim: Recently a novel gene, FAM83H, was identified by a genetic linkage study in the hypocalcified form of the amelogenesis imperfecta family with an autosomal dominant hereditary pattern. Little is known about this novel gene, and so we investigated the expression pattern of Fam83h in murine tooth development using serial sectional in situ hybridisation. Methods and Materials: Using mandibles of ICR mouse at specific developmental stages, in situ hybridisation was performed by DIG-labeled RNA probe. Results: Faint expression was detected in limited cells at embryonic day 14 (E14) in the molar. At the bell stage, E16, Fam83h was localised in the outer and inner enamel epithelium, as well as dental papilla. Fam83h expression begins on E15 in the developing incisor. At E18, Fam83h was expressed in the inner enamel epithelium of the apical bud, ameloblasts and odontoblasts. The expression was stronger in the presecretory stages than the secretory stages. Conclusion: Fam83h was detected in the ameloblasts from the presecretory to the secretory stage, and also the odontoblasts layer and surrounding alveolar bone.

Original languageEnglish
Pages (from-to)846-850
Number of pages5
JournalArchives of Oral Biology
Volume54
Issue number9
DOIs
Publication statusPublished - 2009 Sep 1

Fingerprint

Ameloblasts
Odontoblasts
Dental Enamel
In Situ Hybridization
Tooth
Dental Papilla
Epithelium
Amelogenesis Imperfecta
RNA Probes
Inbred ICR Mouse
Genetic Linkage
Incisor
Mandible
Genes
Bone and Bones

All Science Journal Classification (ASJC) codes

  • Otorhinolaryngology
  • Dentistry(all)
  • Cell Biology

Cite this

Lee, Min Jung ; Lee, Sook Kyung ; Lee, Kyung Eun ; Kang, Hee Yun ; Jung, Hansung ; Kim, Jung Wook. / Expression patterns of the Fam83h gene during murine tooth development. In: Archives of Oral Biology. 2009 ; Vol. 54, No. 9. pp. 846-850.
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Expression patterns of the Fam83h gene during murine tooth development. / Lee, Min Jung; Lee, Sook Kyung; Lee, Kyung Eun; Kang, Hee Yun; Jung, Hansung; Kim, Jung Wook.

In: Archives of Oral Biology, Vol. 54, No. 9, 01.09.2009, p. 846-850.

Research output: Contribution to journalArticle

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