Expression profile of rrbp1 genes during embryonic development and in adult tissues of Xenopus laevis

Guang Hui Liu, Cheng Zhou Mao, Hai Yan Wu, Deng Cheng Zhou, Jing Bo Xia, Soo-Ki Kim, Dong Qing Cai, Hui Zhao, Xu Feng Qi

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Recent studies suggest that ribosome-binding protein 1 (RRBP1) is involved in multiple diseases such as tumorigenesis and cardiomyopathies. However, its function during embryonic development remains largely unknown. We searched Xenopus laevis database with human RRBP1 protein sequence and identified two cDNA sequences encoding Xenopus orthologs of RRBP1 including rrbp1a (NM_001089623) and rrbp1b (NM_001092468). Both genes were firstly detected at blastula stage 8 with weak signals in animal hemisphere by whole mount in situ hybridization. Evident expression of rrbp1 was mainly detected in cement gland and notochord at neurula and tailbud stages. Heart expression of rrbp1 was detected at stage 36. RT-PCR results indicated that very weak expression of rrbp1a was firstly detected in oocytes, followed by increasing expression until stage 39. Differently, very weak expression of rrbp1b was firstly observed at stage 2, and then maintained at a lower level to stage 17 followed by an intense expression from stages 19–39. Moreover, both expression profiles were also different in adult tissues. This study reports Xenopus rrbp1 expression during early embryonic development and in adult tissues. Our study will facilitate the functional analysis of Rrbp1 family during embryonic development.

Original languageEnglish
Pages (from-to)1-6
Number of pages6
JournalGene Expression Patterns
Volume23-24
DOIs
Publication statusPublished - 2017 Jan 1

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Xenopus laevis
Ribosomes
Transcriptome
Embryonic Development
Carrier Proteins
Xenopus
Blastula
Notochord
Cardiomyopathies
Oocytes
In Situ Hybridization
Carcinogenesis
Complementary DNA
Databases
Polymerase Chain Reaction
Genes
Proteins

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics
  • Developmental Biology

Cite this

Liu, Guang Hui ; Mao, Cheng Zhou ; Wu, Hai Yan ; Zhou, Deng Cheng ; Xia, Jing Bo ; Kim, Soo-Ki ; Cai, Dong Qing ; Zhao, Hui ; Qi, Xu Feng. / Expression profile of rrbp1 genes during embryonic development and in adult tissues of Xenopus laevis. In: Gene Expression Patterns. 2017 ; Vol. 23-24. pp. 1-6.
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Expression profile of rrbp1 genes during embryonic development and in adult tissues of Xenopus laevis. / Liu, Guang Hui; Mao, Cheng Zhou; Wu, Hai Yan; Zhou, Deng Cheng; Xia, Jing Bo; Kim, Soo-Ki; Cai, Dong Qing; Zhao, Hui; Qi, Xu Feng.

In: Gene Expression Patterns, Vol. 23-24, 01.01.2017, p. 1-6.

Research output: Contribution to journalArticle

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AU - Mao, Cheng Zhou

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AU - Xia, Jing Bo

AU - Kim, Soo-Ki

AU - Cai, Dong Qing

AU - Zhao, Hui

AU - Qi, Xu Feng

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