Recent studies suggest that ribosome-binding protein 1 (RRBP1) is involved in multiple diseases such as tumorigenesis and cardiomyopathies. However, its function during embryonic development remains largely unknown. We searched Xenopus laevis database with human RRBP1 protein sequence and identified two cDNA sequences encoding Xenopus orthologs of RRBP1 including rrbp1a (NM_001089623) and rrbp1b (NM_001092468). Both genes were firstly detected at blastula stage 8 with weak signals in animal hemisphere by whole mount in situ hybridization. Evident expression of rrbp1 was mainly detected in cement gland and notochord at neurula and tailbud stages. Heart expression of rrbp1 was detected at stage 36. RT-PCR results indicated that very weak expression of rrbp1a was firstly detected in oocytes, followed by increasing expression until stage 39. Differently, very weak expression of rrbp1b was firstly observed at stage 2, and then maintained at a lower level to stage 17 followed by an intense expression from stages 19–39. Moreover, both expression profiles were also different in adult tissues. This study reports Xenopus rrbp1 expression during early embryonic development and in adult tissues. Our study will facilitate the functional analysis of Rrbp1 family during embryonic development.
Bibliographical noteFunding Information:
This work was supported by grants from National Natural Science Foundation of China (81570222 81270183 and 91649203), Guangdong Natural Science Funds for Distinguished Young Scholar (2014A030306011), Guangdong Science and Technology Planning Project (2014A050503043), New Star of Pearl River on Science and Technology of Guangzhou (2014J2200002), SRF for ROCS, SEM (2013-693), and the Top Young Talents of Guangdong Province Special Support Program (87315007). This work is also supported by grants from the Research Grants Council of Hong Kong (CUHK413/12 and CUHK24100414).
All Science Journal Classification (ASJC) codes
- Molecular Biology
- Developmental Biology