Extensively drug-resistant Escherichia coli sequence type 1642 carrying an IncX3 plasmid containing the blaKPC-2 Gene associated with transposon Tn4401a

Seri Jeong, Jung Ok Kim, Eun Jeong Yoon, Il Kwon Bae, Woonhyoung Lee, Hyukmin Lee, Yongjung Park, Kyungwon Lee, Seok Hoon Jeong

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Background: Extensively drug-resistant (XDR) Enterobacteriaceae carrying the blaKPC gene have emerged as a major global therapeutic concern. The purpose of this study was to analyze the complete sequences of plasmids from KPC-2 carbapenemase-producing XDR Escherichia coli sequence type (ST) 1642 isolates. Methods: We performed antimicrobial susceptibility testing, PCR, multilocus sequence typing (MLST), and whole-genome sequencing to characterize the plasmid-mediated KPC-2-producing E. coli clinical isolates. Results: The isolates were resistant to most available antibiotics, including meropenem, ampicillin, ceftriaxone, gentamicin, and ciprofloxacin, but susceptible to tigecycline and colistin. The isolates were identified as the rare ST1642 by MLST. The isolates carried four plasmids: the first 69-kb conjugative IncX3 plasmid harbors blaKPC-2 within a truncated T n4401a transposon and blaSHV-11 with duplicated conjugative elements. The second 142kb plasmid with a multireplicon consisting of IncQ, IncFIA, and IncIB carries blaTEM-1b and two class 1 integrons. This plasmid also harbors a wide variety of additional antimicrobial resistance genes including aadA5, dfrA17, mph(A), sul1, tet(B), aac(3′)-IId, strA, strB, and sul2. Conclusions: The complete sequence analysis of plasmids from an XDR E. coli strain related to persistent infection showed the coexistence of a blaKPC-2-carrying IncX3-type plas-mid and a class 1 integron-harboring multireplicon, suggesting its potential to cause outbreaks. Of additional clinical significance, the rare ST1642, identified in a cat, could constitute the source of human infection.

Original languageEnglish
Pages (from-to)17-22
Number of pages6
JournalAnnals of laboratory medicine
Volume38
Issue number1
DOIs
Publication statusPublished - 2018 Jan 1

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Escherichia coli
Plasmids
Genes
Pharmaceutical Preparations
Integrons
Multilocus Sequence Typing
meropenem
Ports and harbors
Sequence Analysis
Colistin
Ceftriaxone
Enterobacteriaceae
Ampicillin
Ciprofloxacin
Infection
Gentamicins
Disease Outbreaks
Cats
Genome
Anti-Bacterial Agents

All Science Journal Classification (ASJC) codes

  • Clinical Biochemistry
  • Biochemistry, medical

Cite this

Jeong, Seri ; Kim, Jung Ok ; Yoon, Eun Jeong ; Bae, Il Kwon ; Lee, Woonhyoung ; Lee, Hyukmin ; Park, Yongjung ; Lee, Kyungwon ; Jeong, Seok Hoon. / Extensively drug-resistant Escherichia coli sequence type 1642 carrying an IncX3 plasmid containing the blaKPC-2 Gene associated with transposon Tn4401a. In: Annals of laboratory medicine. 2018 ; Vol. 38, No. 1. pp. 17-22.
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abstract = "Background: Extensively drug-resistant (XDR) Enterobacteriaceae carrying the blaKPC gene have emerged as a major global therapeutic concern. The purpose of this study was to analyze the complete sequences of plasmids from KPC-2 carbapenemase-producing XDR Escherichia coli sequence type (ST) 1642 isolates. Methods: We performed antimicrobial susceptibility testing, PCR, multilocus sequence typing (MLST), and whole-genome sequencing to characterize the plasmid-mediated KPC-2-producing E. coli clinical isolates. Results: The isolates were resistant to most available antibiotics, including meropenem, ampicillin, ceftriaxone, gentamicin, and ciprofloxacin, but susceptible to tigecycline and colistin. The isolates were identified as the rare ST1642 by MLST. The isolates carried four plasmids: the first 69-kb conjugative IncX3 plasmid harbors blaKPC-2 within a truncated T n4401a transposon and blaSHV-11 with duplicated conjugative elements. The second 142kb plasmid with a multireplicon consisting of IncQ, IncFIA, and IncIB carries blaTEM-1b and two class 1 integrons. This plasmid also harbors a wide variety of additional antimicrobial resistance genes including aadA5, dfrA17, mph(A), sul1, tet(B), aac(3′)-IId, strA, strB, and sul2. Conclusions: The complete sequence analysis of plasmids from an XDR E. coli strain related to persistent infection showed the coexistence of a blaKPC-2-carrying IncX3-type plas-mid and a class 1 integron-harboring multireplicon, suggesting its potential to cause outbreaks. Of additional clinical significance, the rare ST1642, identified in a cat, could constitute the source of human infection.",
author = "Seri Jeong and Kim, {Jung Ok} and Yoon, {Eun Jeong} and Bae, {Il Kwon} and Woonhyoung Lee and Hyukmin Lee and Yongjung Park and Kyungwon Lee and Jeong, {Seok Hoon}",
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Extensively drug-resistant Escherichia coli sequence type 1642 carrying an IncX3 plasmid containing the blaKPC-2 Gene associated with transposon Tn4401a. / Jeong, Seri; Kim, Jung Ok; Yoon, Eun Jeong; Bae, Il Kwon; Lee, Woonhyoung; Lee, Hyukmin; Park, Yongjung; Lee, Kyungwon; Jeong, Seok Hoon.

In: Annals of laboratory medicine, Vol. 38, No. 1, 01.01.2018, p. 17-22.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Extensively drug-resistant Escherichia coli sequence type 1642 carrying an IncX3 plasmid containing the blaKPC-2 Gene associated with transposon Tn4401a

AU - Jeong, Seri

AU - Kim, Jung Ok

AU - Yoon, Eun Jeong

AU - Bae, Il Kwon

AU - Lee, Woonhyoung

AU - Lee, Hyukmin

AU - Park, Yongjung

AU - Lee, Kyungwon

AU - Jeong, Seok Hoon

PY - 2018/1/1

Y1 - 2018/1/1

N2 - Background: Extensively drug-resistant (XDR) Enterobacteriaceae carrying the blaKPC gene have emerged as a major global therapeutic concern. The purpose of this study was to analyze the complete sequences of plasmids from KPC-2 carbapenemase-producing XDR Escherichia coli sequence type (ST) 1642 isolates. Methods: We performed antimicrobial susceptibility testing, PCR, multilocus sequence typing (MLST), and whole-genome sequencing to characterize the plasmid-mediated KPC-2-producing E. coli clinical isolates. Results: The isolates were resistant to most available antibiotics, including meropenem, ampicillin, ceftriaxone, gentamicin, and ciprofloxacin, but susceptible to tigecycline and colistin. The isolates were identified as the rare ST1642 by MLST. The isolates carried four plasmids: the first 69-kb conjugative IncX3 plasmid harbors blaKPC-2 within a truncated T n4401a transposon and blaSHV-11 with duplicated conjugative elements. The second 142kb plasmid with a multireplicon consisting of IncQ, IncFIA, and IncIB carries blaTEM-1b and two class 1 integrons. This plasmid also harbors a wide variety of additional antimicrobial resistance genes including aadA5, dfrA17, mph(A), sul1, tet(B), aac(3′)-IId, strA, strB, and sul2. Conclusions: The complete sequence analysis of plasmids from an XDR E. coli strain related to persistent infection showed the coexistence of a blaKPC-2-carrying IncX3-type plas-mid and a class 1 integron-harboring multireplicon, suggesting its potential to cause outbreaks. Of additional clinical significance, the rare ST1642, identified in a cat, could constitute the source of human infection.

AB - Background: Extensively drug-resistant (XDR) Enterobacteriaceae carrying the blaKPC gene have emerged as a major global therapeutic concern. The purpose of this study was to analyze the complete sequences of plasmids from KPC-2 carbapenemase-producing XDR Escherichia coli sequence type (ST) 1642 isolates. Methods: We performed antimicrobial susceptibility testing, PCR, multilocus sequence typing (MLST), and whole-genome sequencing to characterize the plasmid-mediated KPC-2-producing E. coli clinical isolates. Results: The isolates were resistant to most available antibiotics, including meropenem, ampicillin, ceftriaxone, gentamicin, and ciprofloxacin, but susceptible to tigecycline and colistin. The isolates were identified as the rare ST1642 by MLST. The isolates carried four plasmids: the first 69-kb conjugative IncX3 plasmid harbors blaKPC-2 within a truncated T n4401a transposon and blaSHV-11 with duplicated conjugative elements. The second 142kb plasmid with a multireplicon consisting of IncQ, IncFIA, and IncIB carries blaTEM-1b and two class 1 integrons. This plasmid also harbors a wide variety of additional antimicrobial resistance genes including aadA5, dfrA17, mph(A), sul1, tet(B), aac(3′)-IId, strA, strB, and sul2. Conclusions: The complete sequence analysis of plasmids from an XDR E. coli strain related to persistent infection showed the coexistence of a blaKPC-2-carrying IncX3-type plas-mid and a class 1 integron-harboring multireplicon, suggesting its potential to cause outbreaks. Of additional clinical significance, the rare ST1642, identified in a cat, could constitute the source of human infection.

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