Extent of Mycobacterium bovis infection in dairy cattle herds subject to partial culling as determined by an interferon-gamma assay

Sungmo Je, Un Chang Yeo, Taeksun Song, Ki Cheol Kim, Sung Yun Park, Man Jung Kim, Sangnae Cho

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The interferon-gamma (IFN-γ) assay is employed as a complementary diagnostic test for bovine tuberculosis (BTB) in many countries. To simplify this assay, we established a 96-well plate format using the ESAT-6 and CFP-10 antigens and then employed it to determine the extent of Mycobacterium (M.) bovis infection in dairy herds with a history of BTB outbreaks in a country where only selective culling is practiced. The sensitivity and specificity of this IFN-γ assay were 85.9% and 100%, respectively, based on comparison with the conventional single intradermal tuberculin test (SIDT). The IFN-γ assay was also positive in 30.4% and 36.8% of SIDT-negative animals from herds with recent and remote BTB outbreaks, respectively. Of 14 SIDT-negative, IFN-γ positive cattle, five (35.7%) were culture positive and an additional six were positive based on a polymerase chain reaction-based test for M. bovis. Therefore, the IFN-γ assay has the potential to serve as a specific and sensitive test for M. bovis infection in dairy cattle. Further, the results indicated that a substantial portion of SIDT-negative animals in herds with previous BTB outbreaks were actually infected with M. bovis. Accordingly, the present selective-culling strategy may require modifications to include this more sensitive assay.

Original languageEnglish
Pages (from-to)259-265
Number of pages7
JournalJournal of veterinary science
Volume15
Issue number2
DOIs
Publication statusPublished - 2014 Jan 1

Fingerprint

culling (animals)
Mycobacterium Infections
Mycobacterium bovis
Bovine Tuberculosis
Intradermal Tests
interferon-gamma
Tuberculin Test
Interferon-gamma
dairy cattle
tuberculin
bovine tuberculosis
herds
Disease Outbreaks
assays
infection
testing
Routine Diagnostic Tests
Mycobacterium
dairy herds
diagnostic techniques

All Science Journal Classification (ASJC) codes

  • veterinary(all)

Cite this

Je, Sungmo ; Yeo, Un Chang ; Song, Taeksun ; Kim, Ki Cheol ; Park, Sung Yun ; Kim, Man Jung ; Cho, Sangnae. / Extent of Mycobacterium bovis infection in dairy cattle herds subject to partial culling as determined by an interferon-gamma assay. In: Journal of veterinary science. 2014 ; Vol. 15, No. 2. pp. 259-265.
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Extent of Mycobacterium bovis infection in dairy cattle herds subject to partial culling as determined by an interferon-gamma assay. / Je, Sungmo; Yeo, Un Chang; Song, Taeksun; Kim, Ki Cheol; Park, Sung Yun; Kim, Man Jung; Cho, Sangnae.

In: Journal of veterinary science, Vol. 15, No. 2, 01.01.2014, p. 259-265.

Research output: Contribution to journalArticle

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