Extracellular signal-regulated kinase is involved in tumor necrosis factor-α-induced MUC5AC gene expression in cultured human nasal polyp epithelial cells

Young Kim Jin, Chang Hoon Kim, Kyung Su Kim, Yoon Seok Choi, Jeung Gweon Lee, Joo Heon Yoon

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Abstract

Objectives - Mucus secretion and inflammatory cytokines are increased in cases of chronic sinusitis with polyp. In this study we initially investigated the localization of MUC5AC protein in human nasal polyps. We then examined whether tumor necrosis factor-α (TNF-α) induces MUC5AC gene expression and whether extracellular signal-regulated kinase (ERK) plays a role in TNF-α-induced MUC5AC gene expression in cultured human nasal polyp epithelial cells. Finally, we investigated whether MUC5AC and activated ERK are co-expressed in goblet cells. Material and Methods - Human nasal polyp epithelial cells were isolated and subcultured. Passage-2 polyp epithelial cells were treated with 10 ng/ml TNF-α. ERK involvement was determined by Western blotting and MUC5AC gene expression by reverse transcriptase polymerase chain reaction. An ERK kinase assay and an inhibitor study using PD98059 were also performed. Immunohistochemical staining using polyclonal MUC5AC antibody and a phospho-specific ERK antibody was done in nasal polyps. Results - MUC5AC was found to be expressed in the majority of goblet cells in human nasal polyp epithelium. TNF-α induced MUC5AC gene expression, and ERK activation was found to be essential for TNF-α-induced MUC5AC gene expression in cultured human nasal polyp epithelial cells. In addition, both MUC5AC and activated ERK were co-expressed in goblet cells of human nasal polyp epithelium. Conclusions - TNF-α induces MUC5AC gene expression via ERK in cultured human nasal polyp epithelial cells.

Original languageEnglish
Pages (from-to)953-957
Number of pages5
JournalActa Oto-Laryngologica
Volume124
Issue number8
DOIs
Publication statusPublished - 2004 Oct 27

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Nasal Polyps
Extracellular Signal-Regulated MAP Kinases
Tumor Necrosis Factor-alpha
Epithelial Cells
Gene Expression
Goblet Cells
Nasal Mucosa
Polyps
Phospho-Specific Antibodies
Sinusitis
Mucus
Reverse Transcriptase Polymerase Chain Reaction
Phosphotransferases
Western Blotting
Staining and Labeling
Cytokines
Antibodies

All Science Journal Classification (ASJC) codes

  • Otorhinolaryngology

Cite this

Jin, Young Kim ; Kim, Chang Hoon ; Kim, Kyung Su ; Choi, Yoon Seok ; Lee, Jeung Gweon ; Yoon, Joo Heon. / Extracellular signal-regulated kinase is involved in tumor necrosis factor-α-induced MUC5AC gene expression in cultured human nasal polyp epithelial cells. In: Acta Oto-Laryngologica. 2004 ; Vol. 124, No. 8. pp. 953-957.
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title = "Extracellular signal-regulated kinase is involved in tumor necrosis factor-α-induced MUC5AC gene expression in cultured human nasal polyp epithelial cells",
abstract = "Objectives - Mucus secretion and inflammatory cytokines are increased in cases of chronic sinusitis with polyp. In this study we initially investigated the localization of MUC5AC protein in human nasal polyps. We then examined whether tumor necrosis factor-α (TNF-α) induces MUC5AC gene expression and whether extracellular signal-regulated kinase (ERK) plays a role in TNF-α-induced MUC5AC gene expression in cultured human nasal polyp epithelial cells. Finally, we investigated whether MUC5AC and activated ERK are co-expressed in goblet cells. Material and Methods - Human nasal polyp epithelial cells were isolated and subcultured. Passage-2 polyp epithelial cells were treated with 10 ng/ml TNF-α. ERK involvement was determined by Western blotting and MUC5AC gene expression by reverse transcriptase polymerase chain reaction. An ERK kinase assay and an inhibitor study using PD98059 were also performed. Immunohistochemical staining using polyclonal MUC5AC antibody and a phospho-specific ERK antibody was done in nasal polyps. Results - MUC5AC was found to be expressed in the majority of goblet cells in human nasal polyp epithelium. TNF-α induced MUC5AC gene expression, and ERK activation was found to be essential for TNF-α-induced MUC5AC gene expression in cultured human nasal polyp epithelial cells. In addition, both MUC5AC and activated ERK were co-expressed in goblet cells of human nasal polyp epithelium. Conclusions - TNF-α induces MUC5AC gene expression via ERK in cultured human nasal polyp epithelial cells.",
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Extracellular signal-regulated kinase is involved in tumor necrosis factor-α-induced MUC5AC gene expression in cultured human nasal polyp epithelial cells. / Jin, Young Kim; Kim, Chang Hoon; Kim, Kyung Su; Choi, Yoon Seok; Lee, Jeung Gweon; Yoon, Joo Heon.

In: Acta Oto-Laryngologica, Vol. 124, No. 8, 27.10.2004, p. 953-957.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Extracellular signal-regulated kinase is involved in tumor necrosis factor-α-induced MUC5AC gene expression in cultured human nasal polyp epithelial cells

AU - Jin, Young Kim

AU - Kim, Chang Hoon

AU - Kim, Kyung Su

AU - Choi, Yoon Seok

AU - Lee, Jeung Gweon

AU - Yoon, Joo Heon

PY - 2004/10/27

Y1 - 2004/10/27

N2 - Objectives - Mucus secretion and inflammatory cytokines are increased in cases of chronic sinusitis with polyp. In this study we initially investigated the localization of MUC5AC protein in human nasal polyps. We then examined whether tumor necrosis factor-α (TNF-α) induces MUC5AC gene expression and whether extracellular signal-regulated kinase (ERK) plays a role in TNF-α-induced MUC5AC gene expression in cultured human nasal polyp epithelial cells. Finally, we investigated whether MUC5AC and activated ERK are co-expressed in goblet cells. Material and Methods - Human nasal polyp epithelial cells were isolated and subcultured. Passage-2 polyp epithelial cells were treated with 10 ng/ml TNF-α. ERK involvement was determined by Western blotting and MUC5AC gene expression by reverse transcriptase polymerase chain reaction. An ERK kinase assay and an inhibitor study using PD98059 were also performed. Immunohistochemical staining using polyclonal MUC5AC antibody and a phospho-specific ERK antibody was done in nasal polyps. Results - MUC5AC was found to be expressed in the majority of goblet cells in human nasal polyp epithelium. TNF-α induced MUC5AC gene expression, and ERK activation was found to be essential for TNF-α-induced MUC5AC gene expression in cultured human nasal polyp epithelial cells. In addition, both MUC5AC and activated ERK were co-expressed in goblet cells of human nasal polyp epithelium. Conclusions - TNF-α induces MUC5AC gene expression via ERK in cultured human nasal polyp epithelial cells.

AB - Objectives - Mucus secretion and inflammatory cytokines are increased in cases of chronic sinusitis with polyp. In this study we initially investigated the localization of MUC5AC protein in human nasal polyps. We then examined whether tumor necrosis factor-α (TNF-α) induces MUC5AC gene expression and whether extracellular signal-regulated kinase (ERK) plays a role in TNF-α-induced MUC5AC gene expression in cultured human nasal polyp epithelial cells. Finally, we investigated whether MUC5AC and activated ERK are co-expressed in goblet cells. Material and Methods - Human nasal polyp epithelial cells were isolated and subcultured. Passage-2 polyp epithelial cells were treated with 10 ng/ml TNF-α. ERK involvement was determined by Western blotting and MUC5AC gene expression by reverse transcriptase polymerase chain reaction. An ERK kinase assay and an inhibitor study using PD98059 were also performed. Immunohistochemical staining using polyclonal MUC5AC antibody and a phospho-specific ERK antibody was done in nasal polyps. Results - MUC5AC was found to be expressed in the majority of goblet cells in human nasal polyp epithelium. TNF-α induced MUC5AC gene expression, and ERK activation was found to be essential for TNF-α-induced MUC5AC gene expression in cultured human nasal polyp epithelial cells. In addition, both MUC5AC and activated ERK were co-expressed in goblet cells of human nasal polyp epithelium. Conclusions - TNF-α induces MUC5AC gene expression via ERK in cultured human nasal polyp epithelial cells.

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