Extracellular vesicles derived from macrophages display glycyl-tRNA synthetase 1 and exhibit anti-cancer activity

Peter C. Goughnour; Min Chul Park; Sang Bum Kim; Sangmi Jun; Won Suk Yang; Sehyun Chae; Seongmin Cho; Chihong Song; Ji Hyun Lee; Jae Kyung Hyun; Byung Gyu Kim; Daehee Hwang; Hyun Suk Jung; Yong Song Gho; Sunghoon Kim

doi:10.1002/jev2.12029

Extracellular vesicles derived from macrophages display glycyl-tRNA synthetase 1 and exhibit anti-cancer activity

Peter C. Goughnour, Min Chul Park, Sang Bum Kim, Sangmi Jun, Won Suk Yang, Sehyun Chae, Seongmin Cho, Chihong Song, Ji Hyun Lee, Jae Kyung Hyun, Byung Gyu Kim, Daehee Hwang, Hyun Suk Jung, Yong Song Gho, Sunghoon Kim

Department of Pharmacy

Research output: Contribution to journal › Article › peer-review

9 Citations (Scopus)

Abstract

Glycyl-tRNA synthetase 1 (GARS1), a cytosolic enzyme secreted from macrophages, promotes apoptosis in cancer cells. However, the mechanism underlying GARS1 secretion has not been elucidated. Here, we report that GARS1 is secreted through unique extracellular vesicles (EVs) with a hydrodynamic diameter of 20–58 nm (mean diameter: 36.9 nm) and a buoyant density of 1.13–1.17 g/ml. GARS1 was anchored to the surface of these EVs through palmitoylated C390 residue. Proteomic analysis identified 164 proteins that were uniquely enriched in the GARS1-containing EVs (GARS1-EVs). Among the identified factors, insulin-like growth factor II receptor, and vimentin also contributed to the anti-cancer activity of GARS1-EVs. This study identified the unique secretory vesicles containing GARS1 and various intracellular factors that are involved in the immunological defence response against tumorigenesis.

Original language	English
Article number	e12029
Journal	Journal of Extracellular Vesicles
Volume	10
Issue number	1
DOIs	https://doi.org/10.1002/jev2.12029
Publication status	Published - 2020 Oct 1

Bibliographical note

Funding Information:
This work was supported by the Global Frontier Project grant (NRF‐M3A6A4‐2010‐0029785) of the National Research Foundation funded by the Ministry of Science and ICT (MSIT) of Korea. Electron Microscopy study (H.S.J.) was supported by the Global Frontier Research grant (NRF‐2015M3A6A4065729) of the National Research Foundation funded by the Ministry of Science and ICT (MSIT) of Korea.

Publisher Copyright:
© 2020 The Authors. Journal of Extracellular Vesicles published by Wiley Periodicals, LLC on behalf of the International Society for Extracellular Vesicles

All Science Journal Classification (ASJC) codes

Histology
Cell Biology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1002/jev2.12029

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Goughnour, P. C., Park, M. C., Kim, S. B., Jun, S., Yang, W. S., Chae, S., Cho, S., Song, C., Lee, J. H., Hyun, J. K., Kim, B. G., Hwang, D., Jung, H. S., Gho, Y. S., & Kim, S. (2020). Extracellular vesicles derived from macrophages display glycyl-tRNA synthetase 1 and exhibit anti-cancer activity. Journal of Extracellular Vesicles, 10(1), [e12029]. https://doi.org/10.1002/jev2.12029

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abstract = "Glycyl-tRNA synthetase 1 (GARS1), a cytosolic enzyme secreted from macrophages, promotes apoptosis in cancer cells. However, the mechanism underlying GARS1 secretion has not been elucidated. Here, we report that GARS1 is secreted through unique extracellular vesicles (EVs) with a hydrodynamic diameter of 20–58 nm (mean diameter: 36.9 nm) and a buoyant density of 1.13–1.17 g/ml. GARS1 was anchored to the surface of these EVs through palmitoylated C390 residue. Proteomic analysis identified 164 proteins that were uniquely enriched in the GARS1-containing EVs (GARS1-EVs). Among the identified factors, insulin-like growth factor II receptor, and vimentin also contributed to the anti-cancer activity of GARS1-EVs. This study identified the unique secretory vesicles containing GARS1 and various intracellular factors that are involved in the immunological defence response against tumorigenesis.",

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AU - Yang, Won Suk

AU - Chae, Sehyun

AU - Cho, Seongmin

AU - Song, Chihong

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AU - Kim, Byung Gyu

AU - Hwang, Daehee

AU - Jung, Hyun Suk

AU - Gho, Yong Song

AU - Kim, Sunghoon

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N2 - Glycyl-tRNA synthetase 1 (GARS1), a cytosolic enzyme secreted from macrophages, promotes apoptosis in cancer cells. However, the mechanism underlying GARS1 secretion has not been elucidated. Here, we report that GARS1 is secreted through unique extracellular vesicles (EVs) with a hydrodynamic diameter of 20–58 nm (mean diameter: 36.9 nm) and a buoyant density of 1.13–1.17 g/ml. GARS1 was anchored to the surface of these EVs through palmitoylated C390 residue. Proteomic analysis identified 164 proteins that were uniquely enriched in the GARS1-containing EVs (GARS1-EVs). Among the identified factors, insulin-like growth factor II receptor, and vimentin also contributed to the anti-cancer activity of GARS1-EVs. This study identified the unique secretory vesicles containing GARS1 and various intracellular factors that are involved in the immunological defence response against tumorigenesis.

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Extracellular vesicles derived from macrophages display glycyl-tRNA synthetase 1 and exhibit anti-cancer activity

Abstract

Bibliographical note

All Science Journal Classification (ASJC) codes

UN SDGs

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