Extract of Magnoliae Flos inhibits ovariectomy-induced osteoporosis by blocking osteoclastogenesis and reducing osteoclast-mediated bone resorption

Ah Young Jun, Hyun Jeong Kim, Kwang Kyun Park, Kun Ho Son, Dong Hwa Lee, Mi Hee Woo, Yeong Shik Kim, Sang Kook Lee, Won Yoon Chung

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Bone homeostasis is maintained by a balance between bone resorption by osteoclasts and bone formation by osteoblasts. Osteoporosis occurs when osteoclast activity surpasses osteoblast activity. Pro-inflammatory cytokines stimulate osteoclast differentiation and activity by increasing production of macrophage-colony stimulating factor and receptor activator of nuclear factor-κB ligand (RANKL). In this study, we investigated whether Magnoliae Flos (MF), one of the most commonly used Chinese medicinal herbs for managing rhinitis, sinusitis and headache, could effectively inhibit osteoporosis. In ovariectomized (OVX) mice compared to sham mice, the body weight increased and serum levels of alkaline phosphatase (ALP), tartrate resistant acid phosphatase 5b, calcium, and osteocalcin were significantly elevated. However, orally administrated MF extract substantially inhibited the increased body weight and serum levels of bone turnover markers, without any evidence of tissue toxicity. MF extract treatment significantly reversed the morphometric parameters of ovariectomy-induced bone loss, including trabecular bone volume, thickness, number, separation, and bone density, to almost the same levels of the sham mice. Furthermore, MF extract reduced the RANKL-mediated osteoclast differentiation and bone resorption by inhibiting the activities of matrix metalloproteinases (MMPs) and cathepsin K in mouse bone marrow macrophages. MF extract appeared to increase ALP activity in murine osteoblastic cells. Taken together, MF extract may be a beneficial supplement for the blockade of osteoporosis progression, particularly for the management of postmenopausal osteoporosis.

Original languageEnglish
Pages (from-to)1523-1531
Number of pages9
JournalFitoterapia
Volume83
Issue number8
DOIs
Publication statusPublished - 2012 Dec 1

Fingerprint

Ovariectomy
Osteoclasts
Bone Resorption
Osteogenesis
Osteoporosis
Osteoblasts
Bone and Bones
Alkaline Phosphatase
Macrophage Colony-Stimulating Factor Receptors
Body Weight
Cathepsin K
Postmenopausal Osteoporosis
Bone Remodeling
Sinusitis
Osteocalcin
Medicinal Plants
Rhinitis
Serum
Matrix Metalloproteinases
Bone Density

All Science Journal Classification (ASJC) codes

  • Pharmacology
  • Drug Discovery

Cite this

Jun, Ah Young ; Kim, Hyun Jeong ; Park, Kwang Kyun ; Son, Kun Ho ; Lee, Dong Hwa ; Woo, Mi Hee ; Kim, Yeong Shik ; Lee, Sang Kook ; Chung, Won Yoon. / Extract of Magnoliae Flos inhibits ovariectomy-induced osteoporosis by blocking osteoclastogenesis and reducing osteoclast-mediated bone resorption. In: Fitoterapia. 2012 ; Vol. 83, No. 8. pp. 1523-1531.
@article{4ce0e598a21648c98fcf502892d208eb,
title = "Extract of Magnoliae Flos inhibits ovariectomy-induced osteoporosis by blocking osteoclastogenesis and reducing osteoclast-mediated bone resorption",
abstract = "Bone homeostasis is maintained by a balance between bone resorption by osteoclasts and bone formation by osteoblasts. Osteoporosis occurs when osteoclast activity surpasses osteoblast activity. Pro-inflammatory cytokines stimulate osteoclast differentiation and activity by increasing production of macrophage-colony stimulating factor and receptor activator of nuclear factor-κB ligand (RANKL). In this study, we investigated whether Magnoliae Flos (MF), one of the most commonly used Chinese medicinal herbs for managing rhinitis, sinusitis and headache, could effectively inhibit osteoporosis. In ovariectomized (OVX) mice compared to sham mice, the body weight increased and serum levels of alkaline phosphatase (ALP), tartrate resistant acid phosphatase 5b, calcium, and osteocalcin were significantly elevated. However, orally administrated MF extract substantially inhibited the increased body weight and serum levels of bone turnover markers, without any evidence of tissue toxicity. MF extract treatment significantly reversed the morphometric parameters of ovariectomy-induced bone loss, including trabecular bone volume, thickness, number, separation, and bone density, to almost the same levels of the sham mice. Furthermore, MF extract reduced the RANKL-mediated osteoclast differentiation and bone resorption by inhibiting the activities of matrix metalloproteinases (MMPs) and cathepsin K in mouse bone marrow macrophages. MF extract appeared to increase ALP activity in murine osteoblastic cells. Taken together, MF extract may be a beneficial supplement for the blockade of osteoporosis progression, particularly for the management of postmenopausal osteoporosis.",
author = "Jun, {Ah Young} and Kim, {Hyun Jeong} and Park, {Kwang Kyun} and Son, {Kun Ho} and Lee, {Dong Hwa} and Woo, {Mi Hee} and Kim, {Yeong Shik} and Lee, {Sang Kook} and Chung, {Won Yoon}",
year = "2012",
month = "12",
day = "1",
doi = "10.1016/j.fitote.2012.08.020",
language = "English",
volume = "83",
pages = "1523--1531",
journal = "Fitoterapia",
issn = "0367-326X",
publisher = "Elsevier",
number = "8",

}

Extract of Magnoliae Flos inhibits ovariectomy-induced osteoporosis by blocking osteoclastogenesis and reducing osteoclast-mediated bone resorption. / Jun, Ah Young; Kim, Hyun Jeong; Park, Kwang Kyun; Son, Kun Ho; Lee, Dong Hwa; Woo, Mi Hee; Kim, Yeong Shik; Lee, Sang Kook; Chung, Won Yoon.

In: Fitoterapia, Vol. 83, No. 8, 01.12.2012, p. 1523-1531.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Extract of Magnoliae Flos inhibits ovariectomy-induced osteoporosis by blocking osteoclastogenesis and reducing osteoclast-mediated bone resorption

AU - Jun, Ah Young

AU - Kim, Hyun Jeong

AU - Park, Kwang Kyun

AU - Son, Kun Ho

AU - Lee, Dong Hwa

AU - Woo, Mi Hee

AU - Kim, Yeong Shik

AU - Lee, Sang Kook

AU - Chung, Won Yoon

PY - 2012/12/1

Y1 - 2012/12/1

N2 - Bone homeostasis is maintained by a balance between bone resorption by osteoclasts and bone formation by osteoblasts. Osteoporosis occurs when osteoclast activity surpasses osteoblast activity. Pro-inflammatory cytokines stimulate osteoclast differentiation and activity by increasing production of macrophage-colony stimulating factor and receptor activator of nuclear factor-κB ligand (RANKL). In this study, we investigated whether Magnoliae Flos (MF), one of the most commonly used Chinese medicinal herbs for managing rhinitis, sinusitis and headache, could effectively inhibit osteoporosis. In ovariectomized (OVX) mice compared to sham mice, the body weight increased and serum levels of alkaline phosphatase (ALP), tartrate resistant acid phosphatase 5b, calcium, and osteocalcin were significantly elevated. However, orally administrated MF extract substantially inhibited the increased body weight and serum levels of bone turnover markers, without any evidence of tissue toxicity. MF extract treatment significantly reversed the morphometric parameters of ovariectomy-induced bone loss, including trabecular bone volume, thickness, number, separation, and bone density, to almost the same levels of the sham mice. Furthermore, MF extract reduced the RANKL-mediated osteoclast differentiation and bone resorption by inhibiting the activities of matrix metalloproteinases (MMPs) and cathepsin K in mouse bone marrow macrophages. MF extract appeared to increase ALP activity in murine osteoblastic cells. Taken together, MF extract may be a beneficial supplement for the blockade of osteoporosis progression, particularly for the management of postmenopausal osteoporosis.

AB - Bone homeostasis is maintained by a balance between bone resorption by osteoclasts and bone formation by osteoblasts. Osteoporosis occurs when osteoclast activity surpasses osteoblast activity. Pro-inflammatory cytokines stimulate osteoclast differentiation and activity by increasing production of macrophage-colony stimulating factor and receptor activator of nuclear factor-κB ligand (RANKL). In this study, we investigated whether Magnoliae Flos (MF), one of the most commonly used Chinese medicinal herbs for managing rhinitis, sinusitis and headache, could effectively inhibit osteoporosis. In ovariectomized (OVX) mice compared to sham mice, the body weight increased and serum levels of alkaline phosphatase (ALP), tartrate resistant acid phosphatase 5b, calcium, and osteocalcin were significantly elevated. However, orally administrated MF extract substantially inhibited the increased body weight and serum levels of bone turnover markers, without any evidence of tissue toxicity. MF extract treatment significantly reversed the morphometric parameters of ovariectomy-induced bone loss, including trabecular bone volume, thickness, number, separation, and bone density, to almost the same levels of the sham mice. Furthermore, MF extract reduced the RANKL-mediated osteoclast differentiation and bone resorption by inhibiting the activities of matrix metalloproteinases (MMPs) and cathepsin K in mouse bone marrow macrophages. MF extract appeared to increase ALP activity in murine osteoblastic cells. Taken together, MF extract may be a beneficial supplement for the blockade of osteoporosis progression, particularly for the management of postmenopausal osteoporosis.

UR - http://www.scopus.com/inward/record.url?scp=84870773944&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84870773944&partnerID=8YFLogxK

U2 - 10.1016/j.fitote.2012.08.020

DO - 10.1016/j.fitote.2012.08.020

M3 - Article

C2 - 22981503

AN - SCOPUS:84870773944

VL - 83

SP - 1523

EP - 1531

JO - Fitoterapia

JF - Fitoterapia

SN - 0367-326X

IS - 8

ER -