Extraction of leukocyte in a cell image with touching red blood cells

Chee Sun Won, Jae Yeal Nam, Yoonsik Choe

Research output: Contribution to journalConference article

2 Citations (Scopus)

Abstract

The main difficulty in segmenting a cell image occurs when there are red blood cells touching the leukocyte. Similar brightness of the touched red blood cells with the cytoplasm in the leukocyte makes their separation quite difficult. Conventional approaches were based on the search of the concavities created by the contact points of two round boundaries. That is, a straight line between the two contacting points will be drawn for the separation of the red blood cell from the cytoplasm. In this paper, we propose a new scheme for the separation. We exploit the fact that the boundary of the leukocyte normally has a round shape and a small portion of it is disconnected due to the touching red blood cells. Specifically, at an initial central point of the nucleus in the leukocyte, we can generate the largest possible circle that covers an interior portion of the composite of nucleus and cytoplasm areas. Also, by perturbing the initial central points, we can generate the circles that do not cross the boundary. Then, the union of the interior areas of the circles can cover most of interior regions in the nucleus and the cytoplasm, separating the leukocyte from the touching red blood cells.

Original languageEnglish
Article number43
Pages (from-to)399-406
Number of pages8
JournalProceedings of SPIE - The International Society for Optical Engineering
Volume5672
DOIs
Publication statusPublished - 2005 Jul 20
EventProceedings of SPIE-IS and T Electronic Imaging - Image Processing: Algorithms and Systems IV - San Jose, CA, United States
Duration: 2005 Jan 172005 Jan 18

Fingerprint

leukocytes
Leukocytes
Red Blood Cells
erythrocytes
cytoplasm
Blood
Cells
Cell
Nucleus
cells
Circle
Interior
nuclei
Cover
concavity
unions
Concavity
Point contacts
Brightness
Straight Line

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Computer Science Applications
  • Applied Mathematics
  • Electrical and Electronic Engineering

Cite this

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abstract = "The main difficulty in segmenting a cell image occurs when there are red blood cells touching the leukocyte. Similar brightness of the touched red blood cells with the cytoplasm in the leukocyte makes their separation quite difficult. Conventional approaches were based on the search of the concavities created by the contact points of two round boundaries. That is, a straight line between the two contacting points will be drawn for the separation of the red blood cell from the cytoplasm. In this paper, we propose a new scheme for the separation. We exploit the fact that the boundary of the leukocyte normally has a round shape and a small portion of it is disconnected due to the touching red blood cells. Specifically, at an initial central point of the nucleus in the leukocyte, we can generate the largest possible circle that covers an interior portion of the composite of nucleus and cytoplasm areas. Also, by perturbing the initial central points, we can generate the circles that do not cross the boundary. Then, the union of the interior areas of the circles can cover most of interior regions in the nucleus and the cytoplasm, separating the leukocyte from the touching red blood cells.",
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Extraction of leukocyte in a cell image with touching red blood cells. / Won, Chee Sun; Nam, Jae Yeal; Choe, Yoonsik.

In: Proceedings of SPIE - The International Society for Optical Engineering, Vol. 5672, 43, 20.07.2005, p. 399-406.

Research output: Contribution to journalConference article

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AU - Nam, Jae Yeal

AU - Choe, Yoonsik

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AB - The main difficulty in segmenting a cell image occurs when there are red blood cells touching the leukocyte. Similar brightness of the touched red blood cells with the cytoplasm in the leukocyte makes their separation quite difficult. Conventional approaches were based on the search of the concavities created by the contact points of two round boundaries. That is, a straight line between the two contacting points will be drawn for the separation of the red blood cell from the cytoplasm. In this paper, we propose a new scheme for the separation. We exploit the fact that the boundary of the leukocyte normally has a round shape and a small portion of it is disconnected due to the touching red blood cells. Specifically, at an initial central point of the nucleus in the leukocyte, we can generate the largest possible circle that covers an interior portion of the composite of nucleus and cytoplasm areas. Also, by perturbing the initial central points, we can generate the circles that do not cross the boundary. Then, the union of the interior areas of the circles can cover most of interior regions in the nucleus and the cytoplasm, separating the leukocyte from the touching red blood cells.

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