A new method for the fast extraction of microcystins RR and LR in cyanobacterium was developed using supercritical CO2. The microcystins were successfully extracted with a ternary mixture (90 % CO2, 9.5 % acetic acid. 0.5 % water). The method developed here has several advantages over solid- phase extraction sample preparation for the analysis of microcystins. Sample handling steps are minimized, thus reducing possible losses of analytes and saving analysis time. No organic solvent extractions are involved in this method and no clean-up steps are employed.
Bibliographical noteFunding Information:
This investigation was supported by a grant from Korea Science and Engineering foundation (KOSEF, 97-0501-07-01-3). This work was also supported partially by the Ministry of Education (BSRI-97-3425).
All Science Journal Classification (ASJC) codes
- Analytical Chemistry
- Clinical Biochemistry
- Organic Chemistry