Eye mucosa: An efficient vaccine delivery route for inducing protective immunity

KyoungYul Seo, Soo Jung Han, Hye Ran Cha, Sang Uk Seo, Joo Hye Song, So Hyang Chung, Mi Na Kweon

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

The external part of the eye shares mucosa-associated common characteristics and is an obvious entry site for foreign Ags. We assessed the potential of eyedrop vaccination for effective delivery of vaccines against viral or bacterial infection in mice. Both OVA-specific IgG Ab in serum and IgA Ab in mucosal compartments were induced by eyedrops of OVA with cholera toxin (CT). Eyedrop vaccination of influenza A/PR/8 virus (H1N1) induced both influenza virus-specific systemic and mucosal Ab responses and protected mice completely against respiratory infection with influenza A/PR/8 virus. In addition, eyedrop vaccination of attenuated Salmonella vaccine strains induced LPS-specific Ab and complete protection against oral challenge of virulent Salmonella. Unlike with the intranasal route, eyedrop vaccinations did not redirect administered Ag into the CNS in the presence of CT. When mice were vaccinated by eyedrop, even after the occlusion of tear drainage from eye to nose, Ag-specific systemic IgG and mucosal IgA Abs could be induced effectively. Of note, eyedrops with OVA plus CT induced organogenesis of conjunctiva-associated lymphoid tissue and increased microfold cell-like cells on the conjunctiva-associated lymphoid tissue in the nictitating membrane on conjunctiva, the mucosal side of the external eye. On the basis of these findings, we propose that the eyedrop route is an alternative to mucosal routes for administering vaccines.

Original languageEnglish
Pages (from-to)3610-3619
Number of pages10
JournalJournal of Immunology
Volume185
Issue number6
DOIs
Publication statusPublished - 2010 Sep 15

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Ophthalmic Solutions
Immunity
Mucous Membrane
Vaccines
Cholera Toxin
Conjunctiva
Vaccination
Lymphoid Tissue
Immunoglobulin A
Human Influenza
Immunoglobulin G
Salmonella Vaccines
Nictitating Membrane
H1N1 Subtype Influenza A Virus
Attenuated Vaccines
Organogenesis
Virus Diseases
Orthomyxoviridae
Tears
Nose

All Science Journal Classification (ASJC) codes

  • Immunology

Cite this

Seo, K., Han, S. J., Cha, H. R., Seo, S. U., Song, J. H., Chung, S. H., & Kweon, M. N. (2010). Eye mucosa: An efficient vaccine delivery route for inducing protective immunity. Journal of Immunology, 185(6), 3610-3619. https://doi.org/10.4049/jimmunol.1000680
Seo, KyoungYul ; Han, Soo Jung ; Cha, Hye Ran ; Seo, Sang Uk ; Song, Joo Hye ; Chung, So Hyang ; Kweon, Mi Na. / Eye mucosa : An efficient vaccine delivery route for inducing protective immunity. In: Journal of Immunology. 2010 ; Vol. 185, No. 6. pp. 3610-3619.
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Seo, K, Han, SJ, Cha, HR, Seo, SU, Song, JH, Chung, SH & Kweon, MN 2010, 'Eye mucosa: An efficient vaccine delivery route for inducing protective immunity', Journal of Immunology, vol. 185, no. 6, pp. 3610-3619. https://doi.org/10.4049/jimmunol.1000680

Eye mucosa : An efficient vaccine delivery route for inducing protective immunity. / Seo, KyoungYul; Han, Soo Jung; Cha, Hye Ran; Seo, Sang Uk; Song, Joo Hye; Chung, So Hyang; Kweon, Mi Na.

In: Journal of Immunology, Vol. 185, No. 6, 15.09.2010, p. 3610-3619.

Research output: Contribution to journalArticle

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AU - Seo, KyoungYul

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AU - Kweon, Mi Na

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