Eyedrop vaccination induced systemic and mucosal immunity against influenza virus in ferrets

Sangchul Yoon, Eun Do Kim, Min Suk Song, Soo Jung Han, Tae Kwann Park, Kyoung Sub Choi, Young Ki Choi, Kyoung Yul Seo

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

We investigated eyedrop vaccination (EDV) in pre-clinical development for immunological protection against influenza and for potential side effects involving ocular inflammation and the central nervous system (CNS). Live attenuated influenza EDV, CA07 (H1N1), PZ-4 (H1N2) and Uruguay (H3N2), induced both systemic and mucosal virus-specific antibody responses in ferrets. In addition, EDV resulted in a clinically significant protection against viral challenge, and suppression of viral replication in nasal secretion and lung tissue. Regarding safety, we found that administered EDV flow through the tear duct to reach the base of nasal cavity, and thus do not contact the olfactory bulb. All analyses for potential adverse effects due to EDV, including histological and functional examinations, did not reveal significant side effects. On the basis of these findings, we propose that EDV as effective, while being a safe administration route with minimum local side effects, CNS invasion, or visual function disturbance.

Original languageEnglish
Article numbere0157634
JournalPloS one
Volume11
Issue number6
DOIs
Publication statusPublished - 2016 Jun

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General

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    Yoon, S., Kim, E. D., Song, M. S., Han, S. J., Park, T. K., Choi, K. S., Choi, Y. K., & Seo, K. Y. (2016). Eyedrop vaccination induced systemic and mucosal immunity against influenza virus in ferrets. PloS one, 11(6), [e0157634]. https://doi.org/10.1371/journal.pone.0157634