Fabrication and characterization of optical biosensors using polymer hydrogel microparticles and enzyme-quantum dot conjugates

Sun A. Park, Eunji Jang, Won Gun Koh, Bumsang Kim

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

As a first step in the development of a microanalysis device for the detection of phenol compounds, PHEMA hydrogel microparticles containing conjugates of enzyme (tyrosinase) and quantum dot were fabricated by dispersion photopolymerization and their feasibility as optical biosensors was investigated. The fluorescent response of the PHEMA microparticles containing tyrosinase-quantum dot conjugates decreased with the phenol concentration due to the fluorescence quenching effect caused by the enzyme reaction between tyrosinase and phenol. The results indicate that the activities of tyrosinase and quantum dot were maintained when they were conjugated and the conjugates encapsulated in PHEMA hydrogel microparticles. In conclusion, the PHEMA microparticles containing tyrosinase-quantum dot conjugates prepared in this study have the potential to be used as optical biosensors for the detection of phenol compounds.

Original languageEnglish
Pages (from-to)120-125
Number of pages6
JournalSensors and Actuators, B: Chemical
Volume150
Issue number1
DOIs
Publication statusPublished - 2010 Oct 21

Fingerprint

PHEMA
Polyhydroxyethyl Methacrylate
Monophenol Monooxygenase
Hydrogel
microparticles
bioinstrumentation
Biosensors
Hydrogels
phenols
Semiconductor quantum dots
Phenols
enzymes
Polymers
Phenol
Enzymes
quantum dots
Fabrication
fabrication
polymers
Photopolymerization

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Instrumentation
  • Condensed Matter Physics
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Electrical and Electronic Engineering
  • Materials Chemistry

Cite this

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abstract = "As a first step in the development of a microanalysis device for the detection of phenol compounds, PHEMA hydrogel microparticles containing conjugates of enzyme (tyrosinase) and quantum dot were fabricated by dispersion photopolymerization and their feasibility as optical biosensors was investigated. The fluorescent response of the PHEMA microparticles containing tyrosinase-quantum dot conjugates decreased with the phenol concentration due to the fluorescence quenching effect caused by the enzyme reaction between tyrosinase and phenol. The results indicate that the activities of tyrosinase and quantum dot were maintained when they were conjugated and the conjugates encapsulated in PHEMA hydrogel microparticles. In conclusion, the PHEMA microparticles containing tyrosinase-quantum dot conjugates prepared in this study have the potential to be used as optical biosensors for the detection of phenol compounds.",
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Fabrication and characterization of optical biosensors using polymer hydrogel microparticles and enzyme-quantum dot conjugates. / Park, Sun A.; Jang, Eunji; Koh, Won Gun; Kim, Bumsang.

In: Sensors and Actuators, B: Chemical, Vol. 150, No. 1, 21.10.2010, p. 120-125.

Research output: Contribution to journalArticle

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AB - As a first step in the development of a microanalysis device for the detection of phenol compounds, PHEMA hydrogel microparticles containing conjugates of enzyme (tyrosinase) and quantum dot were fabricated by dispersion photopolymerization and their feasibility as optical biosensors was investigated. The fluorescent response of the PHEMA microparticles containing tyrosinase-quantum dot conjugates decreased with the phenol concentration due to the fluorescence quenching effect caused by the enzyme reaction between tyrosinase and phenol. The results indicate that the activities of tyrosinase and quantum dot were maintained when they were conjugated and the conjugates encapsulated in PHEMA hydrogel microparticles. In conclusion, the PHEMA microparticles containing tyrosinase-quantum dot conjugates prepared in this study have the potential to be used as optical biosensors for the detection of phenol compounds.

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