Fabrication of biofuel cell containing enzyme catalyst immobilized by layer-by-layer method

Kyu Hwan Hyun, Sang Won Han, Won-Gun Koh, Yongchai Kwon

Research output: Contribution to journalArticle

49 Citations (Scopus)

Abstract

Enzymatic biofuel cell (EBC) employing a layer-by-layer (LbL) structure consisting of multiple layers of glucose oxidase (GOx) and poly(ethyleneimine) (PEI) at carbon nanotube (CNT) ([GOx/PEI] n /CNT) is fabricated. The [GOx/PEI] n /CNT serves as anode catalyst for promoting glucose reaction, while Pt is employed as cathode catalyst. To evaluate effect of [GOx/PEI] n /CNT on EBC performance and stability, several characterizations are conducted. The optimal GOx/PEI layer is determined electrochemically, and it turns out that [GOx/PEI] 2 /CNT is the best. Electron transfer rate constant of the optimal layer is 11.3 s -1 , its glucose sensitivity is 83 μAmM -1 cm -2 , and maximum power density of EBC adopting [GOx/PEI] 2 /CNT is 1.34 mWcm -2 . The values are superior to those of other reference structures, indicating that the [GOx/PEI] 2 /CNT can produce excellent reactivity, followed by improved EBC performance. In terms of redox reaction mechanism of flavin adenine dinucleotide (FAD) within [GOx/PEI] 2 /CNT, glucose does not affect the redox reaction of FAD, while oxygen serves as mediator in transferring electrons and protons produced by glucose oxidation into those for reduction reaction of FAD. It is also found that the [GOx/PEI] 2 /CNT is confined by surface reaction and the reaction is quasi-reversible. Regarding long-term stability, [GOx/PEI] 2 /CNT maintains ∼83% of initial activity even after two weeks.

Original languageEnglish
Pages (from-to)197-203
Number of pages7
JournalJournal of Power Sources
Volume286
DOIs
Publication statusPublished - 2015 Jul 15

Fingerprint

Biological fuel cells
Glucose Oxidase
Glucose oxidase
Carbon Nanotubes
glucose
enzymes
oxidase
Enzymes
Carbon nanotubes
catalysts
Fabrication
Catalysts
Enzymatic fuel cells
fabrication
cells
carbon nanotubes
Flavin-Adenine Dinucleotide
Glucose
adenines
Redox reactions

All Science Journal Classification (ASJC) codes

  • Renewable Energy, Sustainability and the Environment
  • Energy Engineering and Power Technology
  • Physical and Theoretical Chemistry
  • Electrical and Electronic Engineering

Cite this

Hyun, Kyu Hwan ; Han, Sang Won ; Koh, Won-Gun ; Kwon, Yongchai. / Fabrication of biofuel cell containing enzyme catalyst immobilized by layer-by-layer method. In: Journal of Power Sources. 2015 ; Vol. 286. pp. 197-203.
@article{ca08f2f044f1497297ef55acc7a7152f,
title = "Fabrication of biofuel cell containing enzyme catalyst immobilized by layer-by-layer method",
abstract = "Enzymatic biofuel cell (EBC) employing a layer-by-layer (LbL) structure consisting of multiple layers of glucose oxidase (GOx) and poly(ethyleneimine) (PEI) at carbon nanotube (CNT) ([GOx/PEI] n /CNT) is fabricated. The [GOx/PEI] n /CNT serves as anode catalyst for promoting glucose reaction, while Pt is employed as cathode catalyst. To evaluate effect of [GOx/PEI] n /CNT on EBC performance and stability, several characterizations are conducted. The optimal GOx/PEI layer is determined electrochemically, and it turns out that [GOx/PEI] 2 /CNT is the best. Electron transfer rate constant of the optimal layer is 11.3 s -1 , its glucose sensitivity is 83 μAmM -1 cm -2 , and maximum power density of EBC adopting [GOx/PEI] 2 /CNT is 1.34 mWcm -2 . The values are superior to those of other reference structures, indicating that the [GOx/PEI] 2 /CNT can produce excellent reactivity, followed by improved EBC performance. In terms of redox reaction mechanism of flavin adenine dinucleotide (FAD) within [GOx/PEI] 2 /CNT, glucose does not affect the redox reaction of FAD, while oxygen serves as mediator in transferring electrons and protons produced by glucose oxidation into those for reduction reaction of FAD. It is also found that the [GOx/PEI] 2 /CNT is confined by surface reaction and the reaction is quasi-reversible. Regarding long-term stability, [GOx/PEI] 2 /CNT maintains ∼83{\%} of initial activity even after two weeks.",
author = "Hyun, {Kyu Hwan} and Han, {Sang Won} and Won-Gun Koh and Yongchai Kwon",
year = "2015",
month = "7",
day = "15",
doi = "10.1016/j.jpowsour.2015.03.136",
language = "English",
volume = "286",
pages = "197--203",
journal = "Journal of Power Sources",
issn = "0378-7753",
publisher = "Elsevier",

}

Fabrication of biofuel cell containing enzyme catalyst immobilized by layer-by-layer method. / Hyun, Kyu Hwan; Han, Sang Won; Koh, Won-Gun; Kwon, Yongchai.

In: Journal of Power Sources, Vol. 286, 15.07.2015, p. 197-203.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Fabrication of biofuel cell containing enzyme catalyst immobilized by layer-by-layer method

AU - Hyun, Kyu Hwan

AU - Han, Sang Won

AU - Koh, Won-Gun

AU - Kwon, Yongchai

PY - 2015/7/15

Y1 - 2015/7/15

N2 - Enzymatic biofuel cell (EBC) employing a layer-by-layer (LbL) structure consisting of multiple layers of glucose oxidase (GOx) and poly(ethyleneimine) (PEI) at carbon nanotube (CNT) ([GOx/PEI] n /CNT) is fabricated. The [GOx/PEI] n /CNT serves as anode catalyst for promoting glucose reaction, while Pt is employed as cathode catalyst. To evaluate effect of [GOx/PEI] n /CNT on EBC performance and stability, several characterizations are conducted. The optimal GOx/PEI layer is determined electrochemically, and it turns out that [GOx/PEI] 2 /CNT is the best. Electron transfer rate constant of the optimal layer is 11.3 s -1 , its glucose sensitivity is 83 μAmM -1 cm -2 , and maximum power density of EBC adopting [GOx/PEI] 2 /CNT is 1.34 mWcm -2 . The values are superior to those of other reference structures, indicating that the [GOx/PEI] 2 /CNT can produce excellent reactivity, followed by improved EBC performance. In terms of redox reaction mechanism of flavin adenine dinucleotide (FAD) within [GOx/PEI] 2 /CNT, glucose does not affect the redox reaction of FAD, while oxygen serves as mediator in transferring electrons and protons produced by glucose oxidation into those for reduction reaction of FAD. It is also found that the [GOx/PEI] 2 /CNT is confined by surface reaction and the reaction is quasi-reversible. Regarding long-term stability, [GOx/PEI] 2 /CNT maintains ∼83% of initial activity even after two weeks.

AB - Enzymatic biofuel cell (EBC) employing a layer-by-layer (LbL) structure consisting of multiple layers of glucose oxidase (GOx) and poly(ethyleneimine) (PEI) at carbon nanotube (CNT) ([GOx/PEI] n /CNT) is fabricated. The [GOx/PEI] n /CNT serves as anode catalyst for promoting glucose reaction, while Pt is employed as cathode catalyst. To evaluate effect of [GOx/PEI] n /CNT on EBC performance and stability, several characterizations are conducted. The optimal GOx/PEI layer is determined electrochemically, and it turns out that [GOx/PEI] 2 /CNT is the best. Electron transfer rate constant of the optimal layer is 11.3 s -1 , its glucose sensitivity is 83 μAmM -1 cm -2 , and maximum power density of EBC adopting [GOx/PEI] 2 /CNT is 1.34 mWcm -2 . The values are superior to those of other reference structures, indicating that the [GOx/PEI] 2 /CNT can produce excellent reactivity, followed by improved EBC performance. In terms of redox reaction mechanism of flavin adenine dinucleotide (FAD) within [GOx/PEI] 2 /CNT, glucose does not affect the redox reaction of FAD, while oxygen serves as mediator in transferring electrons and protons produced by glucose oxidation into those for reduction reaction of FAD. It is also found that the [GOx/PEI] 2 /CNT is confined by surface reaction and the reaction is quasi-reversible. Regarding long-term stability, [GOx/PEI] 2 /CNT maintains ∼83% of initial activity even after two weeks.

UR - http://www.scopus.com/inward/record.url?scp=84926059183&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84926059183&partnerID=8YFLogxK

U2 - 10.1016/j.jpowsour.2015.03.136

DO - 10.1016/j.jpowsour.2015.03.136

M3 - Article

AN - SCOPUS:84926059183

VL - 286

SP - 197

EP - 203

JO - Journal of Power Sources

JF - Journal of Power Sources

SN - 0378-7753

ER -