Fabrication of carbohydrate chips and their use to probe protein-carbohydrate interactions

Sungjin Park, Myung Ryul Lee, Injae Shin

Research output: Contribution to journalArticle

50 Citations (Scopus)

Abstract

Carbohydrate microarrays have received considerable attention as an advanced technology for the rapid analysis of carbohydrateprotein interactions. This protocol provides detailed procedures for the preparation of carbohydrate microarrays by immobilizing hydrazide-conjugated carbohydrates on epoxide-derivatized glass slides. In addition, we describe the use we make of these microarrays in glycomics research. Unlike other techniques that require large amounts of samples and long assay times, carbohydrate microarrays are used to carry out the rapid assessment of a number of carbohydrate-recognition events with tiny amounts of carbohydrate samples. Furthermore, the microarray technology is also utilized for the rapid assay of enzyme activities. We are able to routinely prepare carbohydrate microarrays within 12 h by using hydrazide-conjugated carbohydrates and apply these microarrays for the studies of glycan-protein interactions within 8 h.

Original languageEnglish
Pages (from-to)2747-2758
Number of pages12
JournalNature Protocols
Volume2
Issue number11
DOIs
Publication statusPublished - 2007 Nov 15

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)

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